clinical coordinator, Research/lab coordinator, data analyst
Resume:
Sheela P. Singh, Ph.D
**************@*****.***
Synopsis/Objective
A highly focused, meticulous scientist with extensive experience in molecular and cancer biology; equipped with wide ranging research background from bacterial to mammalian cells and finally to animal models; possesses outstanding ability to analyze, gather, write, evaluate and produce detailed reports on critical projects.
I am looking for a challenging opportunity in a dynamic organization that can leverage my extensive and varied scientific background and also afford me the chance to grow, both professionally and personally. Even though my experience has largely been molecular and cancer biology focused, I am ready, willing and able to adapt and translate my rich scientific experience to allied or even different areas of research and clinical excellence. Education / Credentials
Ph.D., Molecular Biology, Center for Advanced Studies (CAS) in Botany, University of Madras, India. Aug 1993-May 1999.
Dissertation: Cloning, sequencing and expression of arsenic resistance gene from a marine isolate Pseudomonas fluorescens strain MSP3. M.Phil., Molecular Plant Pathology, CAS in Botany, University of Madras, India. June 1992-Aug 1993.
Dissertation: Isolation of plasmids and cloning of DNA fragment involved in extracellular lipase production from Xanthomonas campestris pv. sesami. M.Sc., Plant Science, CAS in Botany, University of Madras, Chennai, India. June 1990-May1992.
B.Sc., Botany, Women’s Christian College, Chennai, India. May 1987-May 1990.
Dissertation: Studies on host specificity and antibiotic resistance of Rhizobium sp. isolated from WCC campus.
Research Experiences
Research Scientist Dept. of Cancer Systems Imaging, University of Texas, MD Anderson Cancer Center, Texas, USA. 06/2004 - present.
Post-doctoral fellowship funded by National Institute of Health (NIH), Dept. of Experimental Radiation Oncology, University of Texas, MD Anderson Cancer Center, Texas, USA. 1999 - 2004.
Project Associateship, National Institute of Immunology, New Delhi, India. 1998 - 1999. Senior Research Fellowship funded by Council of Scientific and Industrial Research (CSIR), Govt. of India, New Delhi, India. 1995 - 1998.
Junior research Fellowship funded by Department of Ocean Development (DOD), Govt. of India, New Delhi, India. 1994 - 1995.
Senior Research Fellowship funded by Indian Council of Industrial Research (ICAR), Govt. of India, New Delhi, India. 1993.
Competencies
Thorough knowledge of Current Education and Instructional Theories
Remarkable ability to Coordinate Projects and Programs
Outstanding ability to organize, maintain records and evaluate results
Strong communication, problem solving, priority setting skills
Mentored fellow students and research assistants
Provided leadership and guidance for medical seeking students
Collaborated with several departments with projects and expediting scheduled strategies
Assisted in designing and implementing professional development
Maintained timely written and oral communications with all departments
Ensured to implement applicable policies, procedures, and project requirements Technical Skills
1. Imaging Techniques and animal manipulations: In vivo optical imaging, MRI and XSPECT/CT for gene transfer. Developing primary cells / fibroblasts from mouse skin, T cells from mouse spleen for therapy and human PBMCs.
2. Recombinant DNA Techniques: Gene cloning, Gene transfer and therapy, Gene characterization & expression, general molecular biology techniques, Pulse field gel electrophoresis (PFGE).
3. Virology: Adenoviral, Lentiviral and Retroviral construction. 3. Biochemical Techniques: HPLC, GLC, SDS-Polyacrylamide gel electrophoresis of total cell proteins, Expression and Purification of recombinant protein using Ni-NTA resin column, Protein estimation, Agarose gel electrophoresis, radioactive labeling techniques to study the uptake and efflux mechanisms in arsenic resistance. 4. Immunological Technique: Western Blotting, ELISA, Immunofluorescence, Immunohistochemistry.
5. Cell Biology: Tissue culture- human cell lines, growth, maintenance, stables and transfections, Human mesenchymal stem cells and ES cells.
6. Microbiological and Plant Pathological Techniques: Isolation and identification of bacteria, pure culture. Pathogenicity tests, Agricultural microbiological techniques and bactericide evaluation against aerobic and anaerobic bacteria for agricultural marketing. 7. Developmental biological Techniques: Worked with Drosophila melanogaster: In-situ hybridization and antibody staining.
Publications:
1. Ravoori MK, Singh S, Bhavane R, Sood AK, Anvari B, Bankson J, Annapragada A, Kundra V. 2016. Multimodal Magnetic Resonance and Near-Infrared-Fluorescent Imaging of Intraperitoneal Ovarian Cancer Using a Dual-Mode-Dual-Gadolinium Liposomal Contrast Agent. Sci Rep. Dec 22; 6:38991. 2. Singh SP, Ravoori MK, Dixon KA, Han L, Gupta S, Uthamanthil R, Wright KC, Kundra V. 2016. Angiotensin II increases gene expression after selective intra-arterial adenovirus delivery in a rabbit model assessed using in vivo SSTR2-based reporter imaging. EJNMMI Res. 2016 Dec; 6(1):25. Epub 2016 Mar 17.
3. Guerrero YA, Bahmani B, Singh SP, Vullev VI, Kundra V, Anvari B. 2015. Virus- resembling nano-structures for near infrared fluorescence imaging of ovarian cancer HER2 receptors. Nanotechnology. Oct 30; 26(43):435102. Epub 2015 Oct 7. 4. Ravoori MK, Nishimura M, Singh SP, Lu C, Han L, Hobbs BP, Pradeep S, Choi HJ, Bankson JA, Sood AK, Kundra V. 2015. Tumor T1 Relaxation Time for Assessing Response to Bevacizumab Anti-Angiogenic Therapy in a Mouse Ovarian Cancer Model. PLoS One. Jun 22;10(6):e0131095.
5. Bahmani, B, Guerrero Y, Valentine V, Singh SP, Kundra V and Anvari B. 2013. ICG- loaded polymeric nonocapsules functionalized imaging and photodestruction of ovarian cancer cells. Proc. SPIE 8596.
6. Han L, Ravoori M, Wu G, Sakai R, Yan S, Singh S, Xu K, Roth JA, Ji L, Kundra V. 2013. Somatostatin receptor type 2-based receptor expression after plasmid based in vivo gene delivery to non-small cell lung cancer. Molecular Imaging. 12(7): 1 - 10. 7. Ravoori MK, Han L, Singh SP, Dixon K, Duggal J, Liu P, Uthamanthil R, Gupta S, Wright KC, Kundra V. 2013. Non-invasive assessment of gene transfer and expression by in vivo functional and morphological imaging in a rabbit tumor model. PLoS One. 8(6): e62371.
8. Ravoori M, Duggal J, Gagea M, Han L, Singh SP, Liu P, Bankson J, Ma J, Kundra V. 2013. Visualizing the Prostate Gland by MR imaging in Young and Old Mice. PLoS One. 8(3): e55746.
9. Harris DM, Hazan-Haley I, Coombes K, Bueso-Ramos C, Liu J, Liu Z, Li P, Ravoori M, Abruzzo L, Han L, Singh S, Sun M, Kundra V, Kurzrock R and Estrov Z. 2011. Transformation of human mesenchymal cells and skin fibroblasts into hematopoietic cells. PLoS One. 6 (6): e21250.
10. Wright KC, Ravoori MK, Dixon KA, Han L, Singh SP, Liu P, Gupta S, Johnson VE, Kan Z and Kundra V. 2011. Perfusion CT assessment of tissue hemodynamics following hepatic arterial infusion of increasing doses of angiotensin II in a rabbit liver tumor model. Radiology. 260 (3): 718-726.
11. Singh SP, Han L, Murali R, Solis L, Roth J, Ji L, Wistuba I and Kundra V. 2011. SSTR2-Based Reporters for Assessing Gene Transfer into Non-Small Cell Lung Cancer: Evaluation Using an Intrathoracic Mouse Model. Human Gene Therapy. 22(1):55-64. 12. Singh SP, Yang D, Ravoori M, Han L and Kundra V. 2009. In-vivo functional and anatomic imaging for assessment of in-vivo gene transfer. Radiology. 252(3): 763-771. 13. Zhang X, Succi J, Feng Z, Prithivirajsingh S, Story MD and Legerski RJ. 2004. Artemus is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response. Molecular and Cellular Biology. 24: 9207 – 9220. 14. Prithivirajsingh S, Story MD, Bergh SA, Geara FB, Ang K, Ismail SM, Stevens CW, Buchholz TA and Brock WA. 2004. Accumulation of mitochondrial DNA deletion following ionizing radiation. FEBS Letters. 571: 227-232. 15. Ismail SM, Puppi M, Prithivirajsingh S, Munshi A, Raju A, Meyn RE, Buchholz TA, Story MD, Brock WA, Milas L, Thames HD and Stevens CW. 2004. Predicting radiosensitivity using DNA end-binding complex analysis. Clinical Cancer Research. 10: 1226 - 1234.
16. Ismail SM, Prithivirajsingh S, Nimura Y and Stevens CW. 2004. Identification of proteins in the hamster DNA end-binding complex. Intl. J Radiation Biology. 80: 261- 268.
17. Stevens C, Ismail SM, Prithivirajsingh S, Buchholz T and Story MD. 2003. Radiosensitivity can be predicted by DNA-end binding complex analysis. Intl. J. Rad. Onc. Biol. Phy. 57 (2), S204-205.
18. Nimura Y, Prithivirajsingh S, Ismail S, Nairn R, Tucker S, Allen P and Stevens C. 2003. Nucleotide excision repair proteins are important for radiation-enhanced transfection. Intl. J of Radiation Biology. 79(8) 663-669. 19. Chan DW, Chen BP, Prithivirajsingh S, Kurimasa, A, Story, MD, Qin, J and Chen, DJ. 2002. Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks. Genes and Development. 16: 2333 – 2338.
20. Srivastava N, Santhanam R, Sheela P, Mukund S and Gupta, SK. 2002. Evaluation of the immunocontraceptive potential of E. coli expressed recombinant dog ZP2 and ZP3 in a homologous animal model. Reproduction. 123: 847-857. 21. Prithivirajsingh S, Mishra SK and Mahadevan A. 2001. Detection and analysis of chromosomal arsenic resistance in Pseudomonas fluorescens strain MSP3. Biochemical and Biophysical Research Communications. 280(5): 1393 – 1401. 22. Prithivirajsingh S, Mishra SK and Mahadevan A. 2001. Functional analysis of a chromosomal arsenic resistance operon in Pseudomonas fluorescens strain MSP3. Molecular Biology Reports. 28(2): 63 – 72.
23. Srivastava M, Nayak J, Mehrotra V, Kaul R, Sheela P, Gupta SK and Panda AK. 1999. High level expression in Escherichia coli and purification of immunoreactive recombinant bonnet monkey zona pellucida glycoprotein-3 (bmZP3). Process Biochemistry. 35(5): 451 - 457.
24. Gupta, S.K, Govind CK, Senthil D, Srivastava M, Jethanandani P, Kaul R, Mital A, Sivapurapu N and Sheela P. 1999. Molecular characterization of non-human primate zona pellucida glycoprotein. In Reproductive Immunology (ed.). Gupta SK, Narosa Publishing House, New Delhi, India.
25. Santhanam, R, Neela S, Sheela P and Gupta SK. 1998. Molecular cloning, expression and immunological characterization of dog zona pellucida glycoproteins. American J. of Reproductive Immunology, 40(5): 257.
26. Sheela, P, G. Amuthan and A. Mahadevan. 1996. Cloning of Extracellular lipase gene from Xanthomonas campestris pv. sesami on to Escherichia coli. Indian Journal of Experimental Biology. 34: 27 - 31.
27. Sheela, P, G. Amuthan and A. Mahadevan. 1994. Plasmids in Xanthomonas campestris pv. sesami. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz. (J. Plant disease and protection). 101: 482 – 486.
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