Molecular Biologist

CURRICULUM VITAE

NAME: Dr. RAM MOHAN.M

QUALIFICATION:

Ph.D., Biotechnology, Bharathidasan University, Trichirappalli,

Tamilnadu, India.

Title of thesis: Screening, Molecular Characterization and Bioprospecting of a Novel Pseudomonas sp.ram bt-1 isolated from East Coast Region of India.

M. Phil., Zoology (I Class - 1988), Madurai Kamaraj University, Madurai, India.

M.Sc., Zoology, (I Class-1987), Madurai Kamaraj University, Madurai, India.

B.Sc., Zoology, (I Class -1985), Madurai Kamaraj University, Madurai, India.

POSITIONS HELD & TOTAL YEARS OF EXPERIENCE: 19 YEARS

TEACHING EXPERIENCE: 8 Years.

Currently,working as a visiting faculty at Madurai Kamaraj University.

Previous Experience:

i) HOD, Associate Professor, Dept of Biotechnology, Theni College of Arts & Science, Theni, India.(Dec, 2015 – Dec, 2016).

ii) Asst Professor, Dept of Biotechnology, Hindustan College, Coimbatore, India, (Jan, 2015 - Aug, 2015).

iii) Asst Professor, Dept of Biotechnology, New Horizon College of Engineering, Bangalore, India. (Aug, 2014 – Dec, 2014).

iv)Guest Lecturer, Dept of Biotechnology, Bharathidasan University, Tiruchirappalli, India, (Jan, 2013 - March 2014).

v) Asst Professor, Dept of Biotechnology, K.S.R.College, Tiruchengode, India, (Sept, 2006 - June 2011).

RESEARCH EXPEREINCE: 11 Years

i) Research officer - National Cancer Centre, Singapore.

(Feb 2002 – Nov 2005).

Division of Cellular and Molecular Research,

Laboratory of Clinical Pharmacology.

ii) Senior Research

Technician - Department of Genetic Engineering,

School of Biotechnology,

Madurai Kamaraj University,

India. (Dec1994 – Jan 2002).

Research Specialization in Mutagenicity, Cancer Biology and Molecular Biology

Microbiology: Expertise in bacterial molecular biology was Screening, characterization of bacteria, Genomic DNA, Plasmid DNA, and Protein extraction techniques.

Expression of Genes – Southern Hybridization, DNA sequencing- Sanger’s Di - de oxy chain termination - method.

DNA - Polymerase enzyme extracted from Thermus Aquaticus run on SDS –PAGE and Ion exchange chromatography –method was used. Taq-DNA polymerase activity was checked through PCR technique to amplify the gene of interest.

Cancer Molecular biology: At National Cancer Center, Singapore. I have been worked in two different Labs.

1. Cellular and Molecular research Lab: Different type of cell lines cultured and Molecular biology work was carried out for Cancer Gene therapy.

Experiments on Animal model have been carried out for dose fixation of drugs in order to find out the Efficacy and toxicity of drugs.

Tryphan blue assay and Haemocytometer reading was carried out. Western blot, Southern hybridization, Northern Hybridization, CDNA library construction, Mutagenesis - SDM, RFLP, AFLP and SNPs analyzed in different genes (OATP, MDR and CYT p 450- Drug metabolizing enzymes) through Automated DNA sequencer.

The following cell lines namely K562, SW480, Hep3B, HepG2, Hela, 2772, CN2, Fibroblast and Breast cancer cell lines were used in our experimental study.

2. Laboratory of Clinical Pharmacology:

Pharmacokinetics and Pharmacogenomics works have been carried out. To identify the different Exons of different genes Cytochrome p 450, OATP and MDR polymorphic effects in different populations related to ADME approach.

To understand the impact of Tamoxifen drug in different populations of Cancer patients, a study was conducted for the interaction of Drug and DNA using SNP(Single nucleotide polymorphism). SNPs were found in Cyt P 450 exons influencing the efficacy of drugs. My research paper was approved in ASCO (American Scientific Clinical Oncology) conference which was held in Florida, USA.

My Ph.D thesis was on screening of bacterium from Marine Coastal area and it’s characterization using 16s rDNA typing. Phylogenetic tree analysis result was given to NCBI (National Center for Biotechnology Information) to find out the analogues of microorganism/bacteria. The report of NCBI commented that bacterial culture developed by me was a new one even though 60% genome sequence was resembled to Pseudomonas stutzeri.

According to the result of NCBI, synthesis and characterization of the bacteria based on Silver nanoparticles made and the bacteria was named as Pseudomonas rambt-1

Drug target delivery using recent Nano Biotechnology techniques, I have done experiment in Breast Cancer cell line using silver nanoparticles to kill the cancer cells which was followed by tryphan blue assay.

In agriculture, using nanoparticles treatment for killing the pests is highly demanded to overcome the environmental hazards. Silver nanoparticles treatment is one of the approaches to kill the Paracooccus marginatus (papaya mealy bug) pest to find out the mortality rate. I got remarkable result compared with commercial pesticidal treatment.

PROJECTS ACCOMPLISHED:

PROJECT – I

Pharmacogenomics study: Personalised medicine (DRUG - DNA interaction).

SNPs Studies on Cyt-P450, MDR and OATPs families and its pharmacological effects found in different Populations of Cancer Patients (Malaysian, Chinese and Indians) samples using by PCR, RFLP, RT-PCR and DNA sequencing.

PROJECT – II

Cancer Genomics - Aurora kinase (Mitotic spindle regulator - molecular study).

AIK Gene Regulation pathways elucidates in different Cancer cell lines were HeLa, MCF-7, Hep3B, SW480, HepG2, CN-47, 2772, HEK 293 and K562 and Cancer tissues. Molecular analysis of AIK gene Regulation found using by DNA Transfection, Transformation, Southern Hybridisation, Northern hybridization, Western/immunoblot, RT-PCR and DNA Sequencing are employed.

PROJECT -III

Protein purification: Taq Polymerase production at large scale.

Thermus aquaticus/E.Coli culture was grown in 100 Lit, Bio reactor followed by Taq DNA polymerase Enzyme purified by Ion Exchange column chromatography.

PROJECT –IV

Genographic Project: (Migration of Human Genes).

To study the Migration of human genes around the World from east Africa to other parts of the world .There were 10 Lakhs number of Blood samples collected from different races and analyse by PCR followed by DNA Sequencing to find out the Gene marker.

Research Guidance:

Number of M. Phil., Scholars Guided: 3

PUBLICATIONS

Parthasarathy, M. Rammohan, and Karuppiah Balakrishnan, Isolation and Characterization of antimicrobial compound from Marine Streptomyces hygroscopicus BDUS 49. World Journal of Fish and Marine Sciences 4 (3): 268-277 (2012).

M. Rammohan and Karuppiah Balakrishnan. Rapid synthesis and characterization of Silver Nano Particles from Pseudomonas sp.RAM BT-1” Vol 3, No1, Journal of Eco Biotechnology (2011).

Parthasarathy. M. Rammohan. M and Balakrishnan. K, Cytotoxic activity of partially purified 7-demethoxy rapamycin derived from Streptomyces hygrospicus BDUS 49. Int J Med Res.:1(4):198-203. (2011).

M. Rammohan, Balram Chowbay. S and Sreenivas Rao. J Influence of VKORC1 Diplotypes in Cyp2c9 and Cyp2c19 Genotypes on Warfarin disposition and dosage in Chinese Patients, Journal of Clinical Pharmacology and therapeutics- (Communicated).

Pesticidal effects of bio silver nanoparticles on Pest Management – Paracoccous marginatus Journal of Nano research-(communicated).

Anti cancer activity of bio synthesized silver nanoparticles on MCF7 by in vitro. Journal Nanobiotechnology-(Communicated).

INTERNATIONAL CONFERENCE AND WORKSHOPS ATTENDED

1.Paper Presented at AACR International conference – Florida, USA,

Topic: The Influence of functional polymorphisms in UGT1A7 and UGT1A9 on Irinotecan pharmacokinetics in Asian Cancer Patients.

2.Poster & Paper Presented at International conference at Singapore.

Topic: Antimitotic activity and G2 arrest Mediated by HARMINE

3.Responsible care and use of Laboratory Animals course-International workshop at Singapore.

4.Effective working Relationships workshop at Singapore.

5.Performance Management & Appraisal skills workshop at Singapore.

6.International Society for Cancer Gene therapy at Singapore.

7.International conference on Recent advances in Nanotechnology at K.S.R.College, Tiruchengode, India.

NATIONAL WORKSHOP AND CONFERENCE ATTENDED

1.National Workshop on Genome Informatics at Karaikudi, Alagappa University, India.

2.National Workshop on Nanotechnology, K.S.R.college, Tamilnadu, India.

3.Science Day Annual Conferences, Madurai Kamaraj University, India.

Management sponsored Faculty Development Programme, K.S.R.College, Tiruchengode, India.

4.Advanced Molecular Techniques workshop, K.S.R.College, India.

HONOURS and AWARDS RECEIVED:

Best faculty - Management Development Programme, K.S.R.College, Tiruchengode, India.

Social Worker - During SARS at Singapore.

Under Officer - NCC (National cadet corps).

MEMBERSHIP:

(ASCO) American Scientific Clinical Oncology.

Acting Member for Anti ragging Committee.

Book’s Publication:

1. Introduction on Human Pathology under Correction.

2. An Over view of Pharmaceutical Biotechnology under Correction.

REFERENCE

1. Dr.P.S.N.Srinivas,(Vasu)

Director

Campro India Ag Tech Services,

97,Vanagaram Road

Chennai-600095

Mobile +91-994*******

Email:*********@*****.***

************@*****.***

2.Dr.VENKATESAN SUBBUSAMY

Sr.GM-Regulatory Affairs

Nagarjuna Agrichem Limited

Plot No.12- A,”C” Block,Lakshmi Towers,Nagarjuna Hills,Panjagutta,

Hyderabad-500082,India.

Email:***********@*****************.***

Mobile:+91-967*******

3. Dr.Balram C,PhD

Professor

Clinical Pharmacology Laboratory

Division of Medical Sciences

National Cancer Centre Singapore

Singapore 169610

DID: (65) 64368321

Fax: (65) 63720161

Email: ******@****.***.**

CONTACT ADDRESS

Dr. M. RAMMOHAN

7/894 B, Gurusamy Nagar colony,

Nagamalai Pudukkottai.

Madurai- 625021.

INDIA.

E. Mail: *.*********@*****.***

PHONE: +91-709*******, +91-989*******

Contact this candidate