Quality Control Manager

EDUCATION

University of California, Davis, September 2003- June 2007

oB.S. in Genetics with Honors, Minor in Art History

RELEVANT EXPERIENCE AND SKILLS

Nucleic Acid Extraction, Quantification, Quality Testing, and Processing

oSkilled in RNA and DNA manual extractions.

oSkilled in Qubit, Nanodrop, and QPCR quantitation methods.

oExperienced in Agilent Bioanalyzer High Sensitivity chip, DNA 1000 chip, RNA 6000 Pico chip, and RNA 600 Nano chip protocols.

oExperienced with difficult sample types such as, FFPE DNA, WGA DNA, and DNA from laser capture.

oSkilled in DNAse treatment, cDNA synthesis, and pre-amplification protocols.

Quantitative PCR

oExperienced in assay design using Primer Express.

oSkilled in TaqMan and SYBR chemistries for genotyping, DNA quantification, miRNA relative expression, mRNA relative expression, DNA copy number analysis, and absolute quantification analysis.

oSkilled in protocols for the ABI 7900HT, Viia7, QuantStudio 6, Wafergen Smart chip system, LifeTechnologies OpenArray NTcyler platform, and LifeTechnologies OpenArray 12Kflex Quant Studio.

MALDI-TOF Mass Spectrometry

oSkilled in genotyping and mutation detection using the Sequenom Massarray System.

oFamiliar with methylation analysis on Sequenom Massarray System.

oSkilled in Massarray Assay design software for IPlex genotyping.

oDeveloped manual mutation analysis work flow for the Colocarta mutation panel.

Targeted Next Generation Sequencing (NGS)

o5 years experience with the Ion Personal Genome Machine (PGM) platform and protocols.

o1 year experience with the Ion PGMdx platform and protocols.

oFamiliar with the Illumina MiSeq platform and protocols (<1year experience.)

oFamiliar with the Ampliseq assay design.

oSkilled in library preparation, bead based purifications, and library quantitation.

oSkilled in DNA and RNA Oncomine library preparation.

oSkilled in the Ion PGM Template OT2 200 Kit, Ion PGM Sequencing 200 Kit v2, and Sequencing Select protocols

oExperienced in Ion Sphere Quality control Qubit assay and sequencing data quality control.

oExperienced in preliminary downstream analysis using the Torrent Suite, Ion Reporter software, and the Oncomine knowledge data base.

oExperienced with IGV sequencing data review.

Digital PCR

oFamiliar with project design and protocols for the 3D Quantstudio Digital PCR system from LifeTechnologies.

Lab Protocol Development

oExperienced in developing and evaluating new technologies, identifying potential issues, and troubleshooting when designing and adapting new protocols.

Computer Skills

oExperienced in Microsoft Word, Excel, Outlook, and PowerPoint. Familiar with Visual Basic and Excel Macros.

EMPLOYMENT HISTORY

CureSeq, Inc. Brisbane, CA

oR&D Geneticist March 2015-April 2017

Key member of the research and development team focusing on NGS molecular diagnostic and biomarker detection techniques.

Developed products for production and in-house use. Developed DNA quantity and quality assessment kit, and RNA extraction kit.

Optimized and validated a multiplex NGS panel on the Ion PGM.

Maintained lab space, supplies, and equipment.

Carried out contract research projects.

oClinical NGS Laboratory Technician November 2015-January 2017

Setup clinical laboratory for company’s expansion.

Designed and performed validation of lab developed tests.

Accessioned and processed clinical laboratory samples in a CAP and CLIA laboratory.

Maintained clinical lab space, supplies, equipment, and maintenance logs.

Performed regular reagent lot tests.

oClinical Report Review Board Member July 2016-April 2017

Responsible for data analysis review of clinical laboratory sequencing data for quality control.

Reviewed bioinformatics report for creation of the clinical laboratory report.

Responsible for creating the clinical laboratory report, including listing FDA approved drugs and open clinical trials associated with actionable mutations.

oClient Service Manager November 2016-April 2017

Responsible for communications and relations with ordering physicians’ offices, reference laboratories, and pathology laboratories.

Processed test request forms from ordering physician’s office

Requested samples from pathology laboratories.

Collected insurance and billing information for claim submissions.

Ensured clients receive assistance in a helpful, efficient manner.

Genome Analysis Core Facility, UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA

oStaff Research Associate III January 2014-March 2015

oStaff Research Associate II September 2009-January 2014

oStaff Research Associate I September 2007-September 2009

Performed genomic analysis services.

Specialized in quantitative PCR, genotyping, gene expression, and methylation analyses, on various platforms.

Performed library preparation and next generation targeted sequencing on the Ion Torrent Personal Genome Machine Platform.

Responsible for troubleshooting projects and developing procedures and protocols to improve the efficiency and quality of Core generated data.

Provided experimental design recommendations and procedural trainings to the UCSF research community as well as customer support.

Maintained lab space and equipment.

Medical Office of Chuc Van Dang MD, San Leandro, CA

oMedical Biller June 2004 – September 2007

Integrated new web-based platform for electronic billing, increasing productivity and reducing turnaround time for claim payment.

Assisted office manager with data entry and scheduling.

Occasional receptionist.

Law Offices of Chow and Losinski, San Leandro, CA

oRecords Clerk June 2002-September 2003

Performed general office assistance in filing, copying, scanning, and faxing.

Publications:

Dang J, Mendez P, Lee S, et al.: Development of a Robust DNA Quality and Quantity Assessment qPCR Assay for Targeted Next-Generation Sequencing (NGS) Library Preparation. International Journal of Oncology, Oct;49(4):1755-65. doi: 10.3892/ijo.2016.3654. Epub 2016 Aug 11.

https://www.ncbi.nlm.nih.gov/pubmed/27511764

Mendez P, Dang J, Kim JW, et al.: Comprehensive evaluation and validation of targeted next-generation sequencing performance in two clinical laboratories. International Journal of Oncology, 2016 Jul;49(1):235-42. doi: 10.3892/ijo.2016.3497. Epub 2016 Apr 25.

https://www.ncbi.nlm.nih.gov/pubmed/27121194

Poster Presentations:

Dang J, Mendez P, Lee S, et al.: Development of a Robust DNA Quality and Quantity Assessment qPCR Assay for Targeted Next-Generation Sequencing (NGS) Library Preparation.

Association for Molecular Pathology Annual Meeting, Charlotte, NC, 2016

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