Ms Maintenance
Resume:
Oleg Timofeev
** ***** **., ******** ***. NJ ****2
Senior Bio-Analytical Chemist
Bio-analytical chemistry professional with broad experience in bio-analytical assay development and validation and well qualified to fill an industry or academic position in chemistry and MS (mass spectrometry). Published 38 articles in scientific journals and responsible for 3 patents. Innovative chemist with over 20 years of pharmaceutical R & D (Research & Development) experience. Strong analytical methods to determine drugs in biological matrices for NDA (New Drug Application) filings, mass spectrometry of small molecules and peptides, unknown identification by MS and NIST (National Institute of Standards & Technology) MS database.
AREAS OF STRENGTH AND EXPERTISE
Drugs determination in biological tissues: Method development and validation (Extraction and HPLC-MS/MS), Quality control, Stability Testing, Ion source development (Chemical Ionization), High resolution MS, NIST Mass spectrometry Database.
Instrumentation
AB Sciex HPLC-MS
Agilent HPLC-MS
Thermo HPLC-MS
Analyst Software
XCalibur Software
Empower Software
Mass Hunter Software
All known ionization methods (EI, CI, FAB, ESI, APCI, MALDI)
High resolution MS
Thermo Orbitrap
PROFESSIONAL EXPERIENCE
Short-Term Assignments, Various Locations 2009 to Present
Sanofi Pasteur, Swiftwater, PA: 3 Month contract; Marth 2016-May2016.
MonoSol Rx, Warren, NJ: Successfully performed HPLC sample analysis of prescription formulations based on Pharm Film® technology, July 2015-October 2015
Brunswick Laboratories, Westborough, MA: Determined drugs and bio-markers of oxidative stress present in human blood plasma, serum, and urine; developed methods of extraction (LLE); developed and validated HPLC-MS/MS methods; performed sample analysis; performed routine maintenance of the instruments; submitted results to the ASMS conference.
August, 2013 – August, 1014;
Enzon Pharmaceuticals, Piscataway, NJ: Successfully determined PEG-conjugated drugs, by MALDI MS and HPLC-MS; Developed HPLC-MS/MS methods; determined purity of raw materials and final products, (HPLC-UV); performed routine maintenance of the instruments.
November, 2012 – March, 2013;
HFL Sport Sciences, Lexington, KY: Determined prohibited drugs in horse urine and blood samples from racing horses. Developed and validated HPLC-MS/MS methods, (Thermo Orbitrap); performed sample analysis; performed routine maintenance of the instruments.
December, 2011 – April, 1012
Kelly Scientific, Skillman, NJ (J&J): Analyzed finished products and raw materials; developed HPLC-UV methods; utilized HPLC-MS; performed sample analysis; performed routine maintenance of the instruments.
November, 2010 – March, 2011
Synerfac Technical Staffing, Medicia Holding, Dayton, NJ; Developed HPLC-UV methods; performed sample analysis; tested raw material bulk products and finished goods for release and stability in compliance with regulatory guidelines; utilized HPLC-UV; performed routine maintenance of the instruments.
Signum Biosciences, Monmouth Junction, NJ August, 2008 to August, 2009
Analytical Chemist
Developed and validated critically important bio-analytical assays for the determination of lipid-like compounds in mice brain tissue, whole blood and blood plasma.
Performed sample analysis, developed methods for numerous projects including pharmacokinetics, metabolic profiling and tracing of radio-labeled molecules in the biological matrix.
Eurofins Scientific, Dayton, N December, 2006 to April, 2008
Senior Analytical Chemist
Successfully developed and validated methods for the quantification of impurities in herbicide samples.
Used HPLC with UV detector for quantification and HPLC-MS and GC-MS detectors (Thermo Fisher Ion Trap Advantage and Sciex 4000) for qualitative analysis, quantitative analysis and the identification of impurities using a NIST MS database.
Mylan Pharmaceuticals, GMP Laboratory, Morgantown, WV 2002 to 2006
Scientist II
Created and validated methods of generic drug extraction from human blood plasma (LLE, SPE and online extraction) and developed novel HPLC-MS/MS assays for a number of generic drugs.
Utilized automated liquid handling devices, analyzed clinical samples for numerous generic drugs and completed an average of five-to-six yearly drug projects.
Rutgers University, Ernest Mario School of Pharmacy, Piscataway, NJ 2000 to 2002
Research Associate
Effectively determined the presence of Decitabine and Campothecin in dog plasma and for a number of in-vitro projects for various drugs by developing and validating HPLC-MS/MS methods to
Provided analytical and purification support using separation techniques and mass spectrometry for drug discovery.
ABC Laboratories, GLP (Good Laboratory Practices) Laboratory, Columbia, MO 1999 to 2000
Research Scientist
Determined drugs in bio matrices by developing and validating HPLC MS/MS Applied Bio System methods.
Identified metabolites using radio-labeled compounds and ran numerous projects, which enabled the analysis of thousands of clinical samples in collaboration with Schering-Plough.
Washington University, NIH Mass Spectrometry Research Resource, St. Louis, MO 1998 to 1999
Research Associate
Published data in the International Journal of Mass Spectrometry and presented twice at ASMS (American Society for Mass Spectrometry).
Performed analytical mass spectrometry service Protein Identification with Peptide Mass Fingerprinting.
AV Bogatsky Physical Chemistry Institute NAS of Ukraine, Ukraine 1985 to 1998
Scientist, Senior Scientist
Published 38 articles in scientific journals and credited with 3 patents.
Developed the chemical ionization methods by implementing metal ions as structural probes (modified commercially available ion sources).
Launched, maintained and managed the mass spectrometry lab, supervised up to 6 employees and ran multiple projects to determine potential drugs in animal tissues.
Performed method development and sample analysis for numerous projects and institutions of the National Academy of Sciences of Ukraine.
EDUCATION AND TRAINING
Master of Science, Solid State Physics, National University of Ukraine, Odessa, Ukraine
PhD, Physical Chemistry, National Academy of Sciences of Ukraine, Odessa, Ukraine
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