ANNIE PHAM

***** *. ********** ******, *** ***, Aurora, CO 80011 • 626-***-**** • aczf71@r.postjobfree.com

Master student with 4 years of research experience working in cell and cancer biology labs. I am interested in this biotechnology position because I would like to learn more about the application of biological technology applied in industry.

Coursework

Introduction to Linux, R studio, and SQLite Data Analysis

Introduction to Biomedical Data Science

Cell Culture Theory

Biochemistry Series

Introduction to Pathology

Cancer Biology

Hypothesis Testing and Development

Molecular Diagnostics

Molecular Genetics

Statistics for Basic Sciences

Development of Drugs and Biologics

Skills

Beginner in Bash shell scripting

Western blots

Beginner in Bioconductor and QIIME

cell culture

Beginner in R studio

qRT-PCR

Beginner in SQLite

Zebrafish husbandry

Prism GraphPad

Microinjections into zebrafish embryos

Microsoft office (word, excel, powerpoint)

Cloning

Comet Assay

Creation of yeast stains

PCR primer design

Immunohistochemistry

RESEARCH EXPERIENCE

Research Associate – Michael Strong Lab, National Jewish March 2017 – present

My research focus on lung microbiome bioinformatics data of cystic fibrosis patients. Using wrappers such as QIIME to analyze microbiome data.

Research Lab Assistant - Mary Reyland Lab, CU Anschutz March 2016 – March 2017

The loss of Protein Kinase C delta (PKCd) in normal salivary glands protects these cells from radiation-induced cell death. My research is focused on understand the molecular pathway of PKCd in salivary glands that would allow these cells to survive radiation damage.

Optimized current western blot protocol to allow better analysis of high molecular weight phosphorylated proteins

oImproved resolution of high molecular weight proteins around 300-500 KDa

Developed a new protocol to analyze DNA damage using Comet Assay

Planned, executed, and analyzed data for my projects

o1 departmental oral presentation about a molecular pathway that might offer radioprotection for normal salivary glands

oEffective use of GraphPad Prism to perform proper statistical analysis on my data

Rotation Student – Traci Lyons Lab, CU Anschutz November 2015 – February 2016

In the Lyons lab, we found that BC patients with high levels of Semaphorin 7a (Sem7a) had lower survival rate in both Hormone Receptor positive (HR+) and triple negative breast cancer (TNBC). My project analyzes if estrogen and/or progesterone promote Sem7a expression in HR+ cells to result in increased tumor cell invasion.

Data mining for publicly available data on breast cancer patients treated with letrozole, an aromatase inhibitor, to analyze if our protein of interest, semaphorin7a, was upregulated over the course of treatment.

Junior Specialist Researcher – Douglas Kellogg Lab via Post-Baccalaureate Research Education Program (PREP), UC Santa Cruz (UCSC) July 2014 – June 2015

Regulatory mechanisms of cell growth and size regulation are fundamental processes for every cell. Yet these mechanisms remain elusive. In Douglas Kellogg’s lab at UCSC, we analyze cell cycle regulators that modulate growth in budding yeast.

PCR primer design and cloning to generate mutant yeast strains

Mating pheromone synchronized/release time course

Coordination with a Graduate student to address his hypothesis-driven research

Work result in co-author of 1 article in-progress in JCB

Research Assistant – Robert Nissen Lab, CSULA November 2012 – June 2014

A significant proportion of congenial anomalies have jaw defects. Studying basic pathways of craniofacial development will strengthen our understanding of causes in human jaw defects. In Robert Nissen’s lab at CSULA, our goal was to analyze how a single gene, wdr68, plays an integral role in craniofacial development of the zebrafish.

Microinjection of mRNA into zebrafish embryo

Time management skills through managing to perform weekly experiments on top of 16+ credits per quarter of undergraduate courses and holding leadership positions for a social and a philanthropic organization on campus (Earth LA and Circle K International)

Effective communications about my research

o3 poster presentations about my research at local and national Conferences

oExtensive lab notes on my research, which allowed Dr. Nissen to use my data for a publication without further contact about the research I performed

oCo-author of 1 publication in PloS One

AWARDS AND AFFILIATIONS

Student Senate, CU Anschutz September 2016 – present

oStudent representation for the Graduate Programs at the CU Anschutz

Planning and executing fun, interactive, and education events for the student body, such as a Chinese New Year celebration for the student population

Personal Statement Workshop host for CSULA September 2016 and September 2015

oEffective Leadership through working with LSAMP to create this hands-on workshop for undergraduate students interested in applying for Graduate School

oEffective communication with LSAMP coordinator and undergraduate students

Reading and revising students’ personal statements one month before workshop

Explaining effective personal statements contents that Graduate programs are looking for in statements

Post-Baccalaureate Research Education Program (PREP) at UCSC July 2014 – June 2015

oNIH funded research opportunity for post-baccalaureate students located at UCSC. PREP allows students to conduct research with UCSC faculty mentor, courses to prepare for GRE, opportunities to participate in research seminars and journal clubs, and promote travel to conferences to present the research.

Biology Departmental Honors, CSULA June 2014

Honors College, CSULA September 2012 – June 2014

Dean’s List, CSULA 2011 – 2014

Louis Stokes Alliances for Minority Program (LSAMP) October 2012- June 2014

Earth Los Angeles (Earth LA) September 2012 – June 2013

Circle K International September 2010 – June 2013

RESEARCH PRESENTATION

“PKCδ regulates DNA damage response and cell cycle progression in salivary glands.” Spring Rotation Talks at CU Anschutz, Aurora, CO. May 2016.

“Investigating Hormonal Regulation of Sem7a in Breast Cancer.” Winter Rotation Talks at CU Anschutz, Aurora, CO. February 2016.

“Negative Crosstalk Between Progestins and Estrogens in Notch1 Regulation.” Fall Rotation Talks at CU Anschutz, Aurora, CO. November 2015.

“A novel Rts1-dependent checkpoint controls cell size at completion of mitosis.” Society for Advancement of Chicanos and Native Americans in Science (SACNAS), Los Angeles, CA. October 2014.

“Epistatic analysis of wdr68 and endothelin-1 (edn1) in first arch patterning in Danio rerio” Annie Pham’s honors defense for CSULA, Los Angeles, CA. June 2014.

“Epistatic analysis of wdr68 and endothelin-1 (edn1) in first arch patterning in zebrafish.” California State University (CSU) Honors Consortium Conference, Fullerton, CA. May 2014.

“Analysis of wdr68 function in craniofacial patterning in the zebrafish.” Southern California Conferences for Undergraduate Research (SCCUR), Whittier, CA. November 2013.

EDUCATION

University of Colorado Anschutz Medical Campus (CU Anschutz) June 2017 (Expected)

Masters in Biomedical Sciences and Biotechnology GPA: 3.55

California State University, Los Angeles (CSULA) September 2010 – June 2014

Bachelor of Science in Biology, Cum Laude GPA: 3.77

RELATED EXPERIENCE

Clinical Care Extender Internship at Queen of the Valley July 2012 - August 2013

Assisted Hospital staff with patient care, on nursing floor and Mother Baby Care Unit

Shadowed clinical laboratory scientists in Lab department

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