JINGJING WANG

***** ****** **** **** **, San Diego, CA 92126 310-***-**** aczexv@r.postjobfree.com EDUCATION

University of California, Los Angeles (UCLA) Sep. 2010 - Dec. 2013 M.S. with three years Research Associate experience, Chemical and Biomolecular Engineering • GPA 3.7/4.0 Nanjing University (NJU) Sep. 2006 - Jun. 2010

B.S. with 2 years Lab Assistant experience, Chemistry and Chemical Engineering • GPA 4.6/5.0 (Rank 1/196) QUALIFICATION SUMMARY

• Skilled in molecular biology techniques including single and multichannel pipettes, primer design, cloning, PCR/RT-PCR, centrifugation, DNA/RNA purification, gel electrophoresis, cell culture, enzyme kinetics, SDS page, in vivo sample preparation, protein expression, Next Gen Sequencing (NGS) etc.

• Extensive experience in reagents development for DNA/RNA isolation and purification.

• Extensive experience in quality control (QC) and technical support through emails/phone calls.

• Proficient in protein purification and biochemical in vitro assays.

• Expert in analytical instruments and knowledge including HPLC, LC-MS, NMR, GC, GC-MS and etc.

• Hands-on experience in stock solution preparation, chemicals ordering and chemical/ biological waste disposal.

• Basic and growing knowledge in python.

• Academic and industry experience in GLP/QSR/BSL2 lab with good understanding of SOPs.

• Extensive report writing, documentation and presentation skills based on experimental results. WORK EXPERIENCE

Zymo Research Corp. Feb. 2014 – Jan. 2017

Research Associate (Full time)

Fully responsible for DNA/RNA purification column kits development, product release as well as product marketing.

Job functions include:

• Patent searching and develop reagents for DNA/RNA column purification from body fluids

(including plasma, serum, amniotic fluid, cerebrospinal fluid, whole blood, saliva, etc).

• Optimize reagents for cell-free and total DNA isolation from large volume of body fluids.

• Reagents stabilization testing, column optimization, quality control of reagents and columns, etc.

• Wrote project progress, compiled project results and delivered presentations to supervisors.

• Product release and product marketing together with both marketing and manufacturing teams. Fully responsible for magnetic bead based automation kit development of cell-free DNA purification from large volume of body fluids.

Job functions include:

• Developed reagents for cell-free DNA magnetic bead purification from large volume of body fluids.

• Design methodology and search potential solutions for fully automated solution starting from large volume of body fluids.

• Collaborated with mechanical engineers regarding automation machine design as well as adaptable machine solutions.

• Wrote project progress, compiled project results and delivered presentations to supervisors.

• Product release and product marketing together with both marketing and manufacturing teams.

Partially responsible for common company supporting. Job functions include:

• Performed quality control bench work for company reagents, enzymes, columns etc.

• Responsible for technical support including emails and phones.

• Collaborate with R&D, manufacturing, customer service and marketing teams for specific customer related inquires.

• Help communication between US side and China manufactures regarding projects project, project specifications and documents translation.

• Responsible for some instrument purchasing and public chemical stock solutions. ACADEMIC EXPERIENCE

Chemical and Biomolecular Engineering Department, UCLA Sep. 2010 - Jun. 2013 Project leadership responsibilities: Lead a cross-functional team whose goal is to unveil the programming rules in iterative fungal polyketide synthase for natural novel small molecules biosynthetic pathway.

• Key personnel involved in coordinating molecular biology with chemistry teams.

• Designed methodology, wrote project progress, compiled project results and delivered presentations to supervisor and external key opinion leaders.

• Led the work on large scale polyketide synthase protein purification for in vitro C13-substrate assays.

• Uncovered highly reducing polyketide synthase programming rules with substrate mimics of intermediates from biosynthetic pathway.

• Write and present abstracts and manuscripts for publication at peer reviewed journal J. Am. Chem. Soc.[1]

Project collaboration responsibilities: Cooperated in a cross-functional team whose goal is to confirm the stereospecificity preference of highly reducing polyketide synthase from protein domain structure view.

• Plasmid construction/expression for ketoreductase and in vitro substrate stereoselectivity

• Unveiled the stereospecificity of single ketoreductase controlled by substrate length.

• Characterized the diastereomer product by prep-HPLC isolation, LCMS, and 1D, 2D - NMR.

• Verified the key residues for diastereomer selectivity by mapping the ketoreductase structural domains.

• Co-authored a journal paper in Nat. Chem. Bio.[2] Project leadership responsibilities: identify biosynthetic pathway of two small bioactive molecules: cytochalasin E and chaetoglobosin A, which are potential anti-tumor drugs.

• Pharmacokinetic characterization of novel small molecules targeting anti-tumor drugs, using pharmacological and genetic techniques.

• Identified and characterized both bioactive molecules from Aspergillus clavatus and Chaetomium globosum under optimized fermentation conditions.

• Key personnel in proposing biosynthetic pathways for both molecules and designing fluent experimental process.

• Primer design, PCR amplification of gene inactivation cassettes, Baeyer-Villiger (BV) gene knockouts of hypothetical functional genes.

• Provided technical support to the chemistry team in substrate synthesis of cytochalasin and chaetoglobosin ketone derivatives for future in vitro assays.

• Design primers, amplification of expression plasmid, sequence identification of expression plasmid, expression of important BV protein and in vitro function identification with synthesized substrates.

• Improved the conversion fourfold excess compared to the original yield of BV protein with assays/ LCMS data analysis and optimized assay conditions for future large scale in vitro purification. Chemistry and Chemical Engineering Department, Nanjing University Sep. 2008 - Jun. 2010 Project assistantship responsibilities: Research assistant in organic synthesis of 1, 8-bis (benzyloxy)-3, 6- diiodonaphthalene.

• Lab supply ordering management and documentation of chemicals.

• Proposed synthesis pathway and led two research assistants for experiments exploration.

• Organic synthesis and product purification with TLC, silica column and prep-HPLC.

• Complied experimental results and presented supervisors and senior lab research mentors.

• Co-authored a journal paper in Acta Crystallogr. [3] PUBLICATIONS

1. Gao, Z., Wang, J., Norwqay, A. K., Qiao, K., Tang, Y., Vederas, J. C. “Investigation of Fungal Iterative Polyketide Synthase Functions Using Partially Assembled Intermediates” J. Am. Chem. Soc. 2013, 135, 1735-1738 (First author equal contribution)

URL: http://pubs.acs.org/doi/abs/10.1021/ja4001823 2. Zhou, H., Gao, Z., Qiao, K., Wang, J., Vederas, J. C., Tang, Y. “A fungal ketoreductase domain that displays substrate-dependent stereospecificity” Nat. Chem. Biol. 2012, 9, 331-333. URL: http://www.nature.com/nchembio/journal/v8/n4/full/nchembio.912.html 3. Hu, Y., Dietrich, D., Xu, W., Thuss, J., Wang, J., Yin, W., Qiao, K., Vederas, J., Tang, Y. “ A carbonate-forming Baeyer-Villiger Monooxygenase” Nat. Chem. (Manuscript submitted ) 4. Liu, Y., Wang, L. Y.; Wang, J. J.; Liu, L.; Teng, M. Y. “1,8-Bis(benzyloxy)-3,6-diiodonaphthalene” Acta Crystallogr E 2010, 66, 1577.

URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3007043/

Contact this candidate