*** ******* **

Canton, MA *****

Phone: 508-***-****

E"mail:(aczbxg@r.postjobfree.com(

(

Hellen Heap

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Education

Biotechnology and Compliance (Certificate) -2017 Quincy College, Quincy, MA Completed over 500 hours of hands on laboratory time, including:

•( Lean Training

•( Biomanufacturing

o( Xcellerex XDR-10 and Single Use Bioreactors

o( Applikon Traditional Bioreactors

•( Chromatography – Ion Exchange, Affinity, Size Exclusion

•( Protein Purification, TFF, Ultra filtration, DF

•( Enzyme Kinetics

•( cGMP, SOPs, FDA Regulations (21 CFR 210/211), Quality Assurance, Quality Control

•( Validation and Calibration of Equipment (Autoclave, Pipette man and pH Meter)

•( Tissue Culture, Mammalian & Bacterial Cell Culture Scale up, Biosafety cabinets, Aseptic technique

•( Cell counting (Hemocytmeter & Automated)

•( Transformation, DNA Purification (Plasmid and Genomic), Restriction Digestion

•( ELISA, Western Blotting, SDS-PAGE, Agarose Gel Electrophoresis, Bioburden Testing

•( Root Cause Analysis, Quality Systems

•( UV/Vis Spectrophotometry, Protein Quantification, DNA Quantification

•( Buffer and Media Preparation

•( Autoclaving, Micropipeting, Operation, Validation and Calibration of Equipment (pH meter, Pipette, Autoclave)

B.S., Biological Sciences Bachelor’s Degree (in progress), Bridgewater State University, Bridgewater, MA

A.S., Associates Science/ Liberal Arts Degree,2015 Massasoit Community College, Canton MA English as a Second Language Certificate, 2002 Keefe Tech Regional School Framingham, MA A.S., Associates Degree Business Administration, 1997 Catholic University of Goias- Brazil Special

Skills

Languages: Portuguese (Native), English (Fluent), and Spanish (Fluent). Software: IOS, Windows Word, Excel, and Power Point Laboratory Skills:

•( Scale-up (bacterial cell culture)

•( Aseptic technique

•( Gowning

•( Filtration (Filter selection)

•( Sonication

•( Centrifugation

•( Spectrophotometer

•( pH meter

•( Benchtop bioreactors (Applikon)

137 Bolivar St

Canton, MA 02021

Phone: 508-***-****

E"mail:(aczbxg@r.postjobfree.com(

(

•( Chromatography (AKTA training)

•( Environmental monitoring (Bioburden, Viable and non-Viable)

•( TFF (UF/DF)

(

Experience

Cultural Care Au pair Local Childcare Coordinator 12/2014 - Present

•( Follow, guide, and enforce U.S. Department of State Regulations to Host Families and Au pairs participants of a Cultural Exchange/Childcare program

•( Host Families Recruitment, screening, and orientation

•( Ongoing supervision, and support to program participants within designated region according to regulations

Zamitti & Carvalho (Now Carvalho & Roth Orthodontics) Dental Assistant 04/2005-8/2009

•( Provided clinical treatment to orthodontic patients, and assisted the doctors with specialty procedures

•( Laboratory duties: Instrument autoclave and sanitation

•( Maintain a sterile, and clean environment according to regulatory agency guidelines

•( Anticipated, planned and budgeted for inventory needs

•( Helped with recruiting, hiring and developing office staff

•( Supported patients throughout their treatment

Babies R Us Promotional Coordinator 02/2004- 04/2005

•( Organized promotional events that targeted multicultural customers and business within the diverse area of the Framingham, Massachusetts location

•( Planned events for expecting mothers that did not speak English to receive the necessary education on nutrition for their babies and themselves during and after their pregnancy

•( Became an active member of the Brazilian, Russian, and Hispanic community and provided workshops specific to these cultures

Kids R Us Supervisor 12/2002-11/2003

•( Lead a team of 5 Departmental Leads within the retail store Kids R Us Department Lead 01/2001-12/2002

•( Managed and oversaw department projects through completion and management approval

•( Interviewed trained and assigned positions to new hires

•( Supported sales in the development and implementation of programs to enhance sales

•( Conducted regular meetings to discuss sales programs strategies and processes

•( Collaborated with other supervisors to design cross-department efficiency Kids R Us Merchandise Associate 12/1999- 01/2001

•( Maintained specific area of the store daily and made sure areas were stocked and clean.

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