Kristen Maslar

*** * *** ******

Philadelphia, PA 19147

570-***-****

acz9zz@r.postjobfree.com

EDUCATION:

Drexel University Philadelphia, PA

MS, Cancer Biology July 2017

Thesis: “Investigating the Role of MAPKAP Kinase 2- Mediated RECQ1 Phosphorylation in Triple Negative Breast Cancer”

GPA: 3.86

The University of Scranton Scranton, PA

BS, Biochemistry, Cellular and Molecular Biology May 2015 Honors: Cum Laude

GPA: 3.61

RESEARCH EXPERIENCE:

Drexel University Philadelphia, PA

Graduate Researcher August 2016-Present

Advisor: Todd Strochlic, VMD, PhD August 2015-December 2015

Conducted independent research using phosphoproteomics to validate RECQ1 (RecQ helicase like protein) as a substrate of MK2

Performed a biochemical assay using purified proteins involving techniques such as: western blot, in vitro kinase assay, and mass spectrometry

Validated that RECQ1 is overexpressed in TNBC compared to normal mammary epithelial cells via western blot analysis.

Tested the effects of chemotherapeutic agents on the p38/MK2 signaling pathway by western blot analysis.

Cultured mammalian breast cancer cell lines for experiments involving plasmid transfection and selective drug treatment.

Optimized expression and purification protocol for recombinant RECQ1. Fox Chase Cancer Center Philadelphia, PA

Rotating Graduate Researcher April 2016-June 2016

Advisor: Jeffry Peterson, PhD

Worked collaboratively to treat triple negative breast cancer cells with over 120 metabolic compounds either alone or in combination with doxorubicin or buthionine sulfoximine and measured changes in cell viability via CellTiter Glo Assay. Drexel University Philadelphia, PA

Rotating Graduate Researcher January 2016-April 2016 Advisor: Michael Bouchard, PhD

Conducted independent research that measured change in protein levels to validate increased AKT activation drives cell proliferation in hepatocellular carcinoma due to HBx expression resulting from HBV replication.

MD Anderson Cancer Center Houston, TX

Summer Research Student June 2014 – August 2014

Advisor: Powel H. Brown, MD, PhD

Performed qRT-PCR, immunofluorescence, cell cycle analysis, and cell viability assays to analyze the effect ASPM (abnormal spindle-like microcephaly-associated protein) on triple negative breast cancer proliferation.

The University of Scranton Scranton, PA

Undergraduate Researcher February 2014 – December 2014 Advisor: Tim Foley, PhD

Performed glutathione assay to determine the role of glucocorticoids in neuroblastomas when exposed to different types and concentrations of oxidative stress. KEY LABORATORY SKILLS:

Molecular Biology

o Primer Design,

Sequencing, &

Sequence Analysis

o Molecular Cloning,

Restriction Enzyme

o PCR, qRT-PCR, Site-

directed Mutagenesis

o Western & Southern

Blot

o DNA Extraction,

Purification, & Gel

Electrophoresis

o Cell Transformations

o Mini and Maxi Prep

o Immunofluorescence

Selection and Screening

o Peptide Screen by

Mass Spectrometry

Cell Science

o Bacterial Cell Culture

o Mammalian Cell

Culture

o Mammalian

Transfection

o CellTiter Glo Assay

o Cell Viability Assay

via Trypan Blue

Specialty Software and

Equipment

o Image J

o GraphPad Prism

Assay Development

o In vitro kinase assay

o Dose dependent drug

treatment

Protein Science

o Affinity Purification

OTHER SKILLS:

Excellent written, documentation, and organizational skills

Team-oriented and receptive to suggestions for improvement

Familiar with analyzing data using common biostatistics tests PRESENTATIONS:

Maslar, K, Haricharan, S, Brown P. “The Role of ASPM in Normal, Pre-Malignant, and Invasive Breast Cancer Cells.” CPRIT Undergraduate Research Program, MD Anderson Cancer Center, Houston, TX, August 2014.

REFERENCES AVAILABLE UPON REQUEST

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