Scientist/Researcher

JIHYUN KIM, Ph.D. SCIENTIST

415-***-**** acz7zn@r.postjobfree.com https://www.linkedin.com/in/JihyunKim916 San Francisco Bay Area

• Extensive hands-on research experience in Cellular and Molecular Biology, Biochemistry, and Genetics.

• Publications in prestigious journals, presentations at national and international meetings, and a prestigious NIH fellowship award (F32) that supported the research.

• Work both independently and in a team environment demonstrated by collaboration and team projects with multidisciplinary team members.

• Strong analytical, problem solving, organizational, and time management skills lead to results that significantly contributes to the overall goals.

HIGHLIGHT OF TECHNICAL SKILLS

• in vivo Models: C. elegans, Drosophila, and mouse

• Molecular Biology: DNA manipulation method (Site directed mutagenesis, cDNA synthesis), DNA/RNA isolation and purification, PCR, RT-PCR/qPCR, DNA cloning, Plasmid construction, Gene cloning and expression, Sample preparation and analysis for NGS (next generation sequence)

• Immunoassays: SDS-PAGE and Western Blot, ELISA, Immunofluorescence, immunohistochemistry, Co-IP, pull-down, Protein expression, purification, and interaction, FACS

• Cell Biology & Cell Culture: Drosophila S2 cells, mammalian cell lines, Primary cell culture and isolation (Human umbilical cord perivascular (HUCPV) cells, mouse Mesenchymal stem cells (MSC), and MEF), human embryonic stem cells, and iPSCs. Transient and stable knockdown/overexpression using RNA and DNA transfection and viral transduction

• Imaging & Instrument: DIC, Brightfield, Confocal & Fluorescence Microscopy (conventional epi-fluorescence, Confocal microscopy, Spinning disk Confocal), Total Internal Reflection Fluorescence microscopy (TIRFM), Two-photon microscopy, Stimulated Emission Depletion (STED) microscopy, FRET, Cellomics High Content (Thermo Fisher), microplate reader, Typhon, Licor, Universal Hood2 Molecular Imager Gel System (Bio-Rad), Flow Cytometry (BD)

• Image Processing & Microscope Software: OpenLab, Image J, Photoshop, Adobe Illustrator, Zeiss Microscope Software

• Data Analysis and software: Gene Ontology analysis and other functional analysis on RNAseq data, DNA sequence analysis software (ApE, Clustal X and W, DNASTAR), FACS analysis software (FlowJo, ModFit), Statistics Software (GraphPad Prism, G power), Data Visualization software (Tableau), knowledge of programming language (Python, Shell Script) and Database (MySQL, Linux)

RESEARCH & TEACHING EXPERIENCE

Biomedical Researcher, Postdoctoral Fellow Mentor, PI: Heinrich Jasper, Ph.D. 2012 – 2016

Buck Institute for Research on Aging Novato, CA

Understand the biology controlling lifespan and advance knowledge leading to healthier lives. Studied adult stem cell proliferation and differentiation and its effect on intestinal regeneration in fruit fly.

Received the Ruth L. Kirschstein National Research Service Award (NRSA) Individual Postdoctoral Fellowship (F32), National Institute of General Medical Sciences.

Discovered the novel regulatory mechanism that regulates differentiation and proliferation of intestinal stem cells.

Lectured Bay Area middle school teachers at STEAM ENGINE 2015.

Developed an interactive game that teachers can apply to their classrooms and is currently in use at area schools.

Mentored and trained undergraduates prepared for medical schools.

Biomedical Researcher, Postdoctoral Fellow, PI: Ze’ev A. Ronai, Ph.D. 2011 – 2012

Sanford Burnham Prebys Medical Discovery Institute La Jolla, CA

Non-profit research facility that blends research with drug discovery to meet clinical needs in the areas of cancer, neuroscience, immunity, and metabolic disorders. Advanced knowledge of skin and liver cancer development.

Discovered context-dependent roles of cell cycle control in cancer development by utilizing mammalian cell culture and mice.

Biomedical Researcher, Postdoctoral Fellow, PI: William L. Stanford, Ph.D. 2009 – 2011

Institute of Biomaterial and Biomedical Engineering University of Toronto, Canada

Conducted studies focusing on stem cell self-renewal and differentiation within this multidisciplinary research unit where researchers collaborate across the disciplines of engineering, medicine, and dentistry to develop innovative solutions for health-care challenges.

Analyzed age-related osteoporosis therapeutics using systems biology and mutant mice.

Studied the pluripotency maintenance of human embryonic stem cells by gene expression profiles.

Doctoral Research Teaching Assistant, PI: Edward T. Kipreos, Ph.D. 2002 – 2009

Cellular Biology University of Georgia Athens, GA

Understand the biological process whose dysfunction leads to pathological conditions. Studied the function of protein degradation in cell cycle regulation in roundworms.

Received Dissertation Completion Award.

Discovered critical roles of protein degradation to prevent genome instability.

Studied the regulation of protein degradation by an inhibitor.

Collaborated with the Gaertig Laboratory to study functions of histone modification in touch receptor neurons.

Lectured and assisted in laboratory classes.

Provided review sessions, mentorship and one-on-one tutoring for undergraduate students to succeed in their classes.

OTHER PROFESSIONAL EXPERIENCE

Associate Editor Bio-Protocol e-Journal 2015 – Present

A peer-reviewed publication that provides high quality, open access protocols for scientists to make life science research more efficient and reproducible.

Recommend protocols for publication and Invite authors to publish their protocols.

Review articles about experiments and protocols, leading to publishing over 15 articles.

Analyst Scientific and Market Life Science Advising (SCIMALS) 2016

A non-profit organization that provides objective third-party scientific and comprehensive market evaluation.

Conducted the secondary research on markets, latest technologies, and competitors with multidisciplinary team members, leading to recommending business strategies to startups.

Event Leads (Leadership team member) Biotech Connection – Bay Area (BCBA) 2015 – 2016

BCBA provides academics and industry professionals with opportunities to work together and to move innovative ideas forward by accessing the business tools and intellectual network.

Orchestrated and executed events for young scientists, graduate students and postdoctoral fellows to gain networking experiences outside of academia.

ORGANIZATIONS (LEADERSHIP & MANAGEMENT)

Membership Committee of San Francisco chapter at Women in Bio 2016 – Present

Program Committee of San Francisco chapter at Association for Women in Science (AWIS) 2014 – 2015

Meeting Reporter of San Diego chapter at Korean-American Women in Science & Engineering 2011 – 2012

EDUCATION

Certificate in Computing Skills for Scientists and Linux Administration 1 2016 – 2017

City College of San Francisco San Francisco, CA

Ph.D. in Cellular Biology University of Georgia Athens, GA 2002 – 2009

Bachelor of Science in Bioscience Korea University Seoul, Korea 1998 – 2001

PRESENTATION (SELECTED)

Oral Presentations:

10th Bi-annual Bay Area Aging Meeting, Novato, CA (2015) Control of the Drosophila Intestinal Stem Cell Proliferation by a New Ubiquitin Ligase Complex

16th International C. elegans Meeting, Los Angeles, CA (2007) (Plenary Session) CUL-4 Prevents DNA Re-replication By Promoting the Phosphorylation-Dependent Nuclear Export of CDC-6 via a CKI-1 Dependent Pathway

Poster Presentations:

Stem Cell Energetics, Berkeley, CA (2014)

55th Annual Drosophila Research Conference, San Diego, CA (2014)

Keystone Symposia on Stem Cell Regulation in Homeostasis and Disease, Banff, Alberta, Canada (2013)

C. elegans Development and Evolution Meeting, Madison, WI (2008)

C. elegans Development and Evolution Meeting, Madison, WI (2006)

15th International C. elegans Meeting, Los Angeles, CA (2005)

East Coast C. elegans Meeting, New Haven, CT (2004)

PUBLICATIONS

Kim, J., Hwangbo, D., Jasper, H. DCAF12 regulates asymmetric cell division and cell cycle exit in the intestinal stem cell lineage. Biology Open (under revision)

Hwangbo, D., Biteau, B., Rath, S., Kim, J., Jasper, H. Control of apoptosis by Drosophila DCAF12. (2016) Developmental Biology 413(1):50-9.

Akella, J.S. *, Wloga, D. *, Kim, J., Starostina, N.G., Lyons-Abbott, S., Morrissette, N.S., Dougan, S.T., Kipreos, E.T., and Gaertig, J. MEC-17 is an alpha-tubulin acetyltransferase. (2010) Nature 467(7312):218-22 *These authors contributed equally to this work

Kim, J. and Kipreos, E.T. Control of the Cdc6 replication licensing factor in metazoa: the role of nuclear export and the CUL4 ubiquitin ligase. (2008) Cell Cycle 7(2):146-150

Kim, J., Feng, H., Kipreos, E.T. C.elegans CUL-4 prevents re-replication by promoting the nuclear export of CDC-6 via a CKI-1-dependent pathway. (2007) Current Biology 17(11):966-72

-Highlighted in Dispatch by Jerome Korzelius and Sander van den Heuvel. (2007) Current Biology 17(16): R630-2

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