RENUKA SABNIS

**** ******* **., ******** ** ***** • 815-***-**** • acz6ql@r.postjobfree.com

PROFILE

Experienced working in laboratory under GLP, GMP and GDP

Skilled in various molecular, cellular, immunology and biochemistry techniques

Highly productive, innovative and result-driven with excellent communication & interpersonal skills

Able to quickly shift domain expertise, team player, and very organized

EDUCATION

MS University of Illinois – Chicago (UIC), College of Medicine, Rockford IL Aug ’15 – Dec ’16

Medical Biotechnology, (cGPA 4.0/4.0), Awarded with MBT one time scholarship grant

BS University of Pune, India Aug ’11 – May ’15

Biotechnology, (cGPA 3.6/4.0)

EXPERIENCE

R&D Intern Thermo Fisher Scientific, Rockford IL May ’16 – Sept ’16

Validated a multiplexed Immunoprecipitation to Mass Spectrometry (IP_MS) assay for targeted protein quantitation of a cancer signaling pathway, for tissue samples.

Developed and optimized a tissue lysate preparation protocol in conformance to the IP_MS workflow.

Maintained cell lines HCT116 and A549 for comparative studies with tissue samples and for analysis of the action of drug inhibition on expression profiles of selected targets in multiplexed IP_MS assay.

Gained a basic understanding of principles and working of Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer and Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer.

Data analysis using Proteome Discoverer, MaxQuant, and Perseus software.

Meticulous notebook maintenance.

Student Researcher UIC-College of Medicine, Rockford IL Sep ’15 – Mar ’16

Dr. Munirathinam Lab, Assisted an ongoing project based on analyzing the oncolytic potential of photodynamic therapy (PDT) via Pheophorbide and 670nm LEDs against prostate cancer for which performed cell culture, different cell-based assays like cell viability, invasion and migration assays and western blot to elucidate mechanistic action of the treatment.

Dr Nitiss Lab, Assisted an ongoing project on the expression of hTOP2 in E.coli and thus worked on insertion of hTOP2 gene in the pET28 vector for transformation in E.coli and gained experience in cloning, PCR, transformation, plasmid extraction and purification, agarose gel electrophoresis, and DNA quantitation.

Undergraduate Researcher University of Pune, India April ’14 – April ’15

The objective of the project was to investigate the increment in nutritional quality of fruit juices after probiotic fermentation using different strains of Lactobacilli and optimize the conditions for production of a functional drink.

Isolated different strains of lactobacilli using selective media, extracted fruit juices, carried out fermentation of fruit juices using these different strains at different conditions and different biochemical assays were used to assess the nutrient content.

Internships India

Nisarga Biotech, Pvt. Ltd. Maharashtra, Gained practical knowledge about principles and working of extraction using supercritical fluid (CO2) plant and various analytical activities involving techniques like GC, HPLC, UV Spectroscopy. March ’15

Sri Sri Institute of Advanced Research, Banglore, Assisted routine microbiological test procedures for quality assessment of pharmaceutical products manufactured in the facility May ’14-June ’14

TECHNICAL SKILLS

Molecular biology skills: DNA/RNA extraction • Cloning and transformation • PCR • Gel Electrophoresis

Mammalian cell culture skills: Growth & maintenance of cell lines • Cell based assays • Cell lysate preparation

Microbiology skills: Antibiotic resistance assays • Bacterial cell culture • Aseptic & sterile techniques • Characterization of microbes of selective media • Streaking techniques

Biochemistry skills: Immunoprecipitation • ELISA • UV Spectroscopy • LC-MS/MS sample preparation • Immunoblotting • Antibody Biotinylation

Computer skills: MS Office • SPSS • Proteome Discoverer • Perseus • T-scratch

EXTRACIRRICULAR ACTIVITIES

Represented Biotechnology as career option at Alignment Academic expo, BMO Harris Bank, Rockford, Fall 2015

Addressed Guilford High school students to introduce Biotechnology profession (National Centre for Rural Health Professions, NCRHP Camp) by conducting laboratory experiments at UIC, Fall 2015

Organized courses and workshops (by Art of Living, NGO) for youth in college campus, 2014

Active volunteer in ‘Vote for better India’ campaign, 2013

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