Professional Experience Laboratory

Location:

Searcy, AR

Posted:

May 02, 2017

Contact this candidate

Resume:

Kathryn L. Smith, MS, PhD

* ********* **., ******, ** 72143 859-***-**** Email: **************@*****.***

Summary of Qualifications

Virologist and researcher with 14 years of academic, laboratory and clinical experience. My passion is disease detection, specifically using molecular assays such as real-time PCR. I have two peer-reviewed publications on the detection and prevalence of EHV-1. I have presented my research at 3 national conferences and one international conference. My laboratory skills include classical virology techniques, such as plaque assays and serum neutralization assays, and molecular techniques, such as conventional and real-time PCR.

Education

PhD in Veterinary Science, University of Kentucky, May 2013

Dissertation: Development of a New Allelic Discrimination Real-Time PCR Assay for the Diagnosis of Equine Herpesvirus-1 and Characterization of the Virulence Determinants of the Virus

MS in Veterinary Science, University of Kentucky, Dec. 2007

Thesis: Tracing the Origin of the Recent Rise in Neuropathogenic EHV-1

BS in Animal Science, Abilene Christian University, May 2001

PROFESSIONAL EXPERIENCE

VMRD Inc., Pullman, WA 2013-2016

2015-2016 Study Director of Services

Coordinate with Quality Control department on:

oUpdating of SOPs

oPreparation for on-site customer audits

oTraceability of documentation

oUpdating training documentation of staff

oError investigations

oReview and implementation of CAPAs

Client interactions:

oOn-site audits of the laboratory

oTechnical guidance regarding test results

oCustom testing per 9CFR, 21CFR &/or EM regulations

Supervisor of training of new technicians:

oUnderstanding and performing lab procedures according to SOPs

oProper documentation of calculations

oProper documentation of laboratory incidents and errors

oProper documentation of routine laboratory procedures

Coordinate with Human Resources to improve employee performances and to address issues within the division

Coordinate with marketing regarding current assays offered by Services and how to better meet the needs of our clients

Documentation:

oPreparation of new SOPs

oEditing of current SOPs per current 9CFR and EM regulations

oReview and sign completed test article and control bench records of 9CFR and EM tests

oReview and sign final reports for release to customers

oReview and sign incident and error reports

oPrepare custom documentation for custom assays

Management of critical lab situations:

oEstablish new work schedule

oInform customers if their testing results are delayed

oCoordinate with Quality Control regarding the generation of incident &/or error reports

oReview and sign amendments to all control and test article documentation

2014 Services Scientist

Testing of various biological specimens for the presence of viruses, according to 9CFR and EM regulations

Training of new technicians

Training of technicians on specialized equipment:

oNanodrop

Lectures on virology

Prepare and maintain cell cultures

Virus Titers

Assay Validations:

oPCR assays

Equipment checks:

oNanodrop

oReal-Time PCR analyzer

Customer Interactions:

oAnswer questions regarding test results

Prepare and Maintain documents according to 9CFR and EM regulations

2013 Validation Technician

Development and Validation of PCR assays

Presentation on assays in development

Bio Safety Officer for Services Division of VMRD (2013-2014):

oWriting of new laboratory biosafety manual

Maxwell H. Gluck Equine Research Center, Lexington, KY 2005-2013

Graduate Research Assistant

Organizations: American Association of Veterinary Laboratory Diagnosticians (2009 - 2012)

Data Analysis:

oGeneration of data charts and graphs

oAnalysis and interpretation of PCR results

Isolation and purification of viral agents from cell cultures

Live animal studies:

oMurine

Inoculation with live virus

Observation

Dissection

Collection and processing of tissues for PCR analysis

oEquine

Inoculation with live virus

Collection and processing of tissues for PCR analysis

Design and optimize of real-time PCR primers and probes

Molecular Biology/Recombinant DNA Techniques

Preparation of manuscripts

oReview and revisions of grant proposals

oWriting and editing of manuscripts for publication

Banfield The Pet Hospital, Augusta, GA 2004-2005

Veterinary Technician

Preparation and administration of vaccines

Blood draws on both dogs and cats

Preparation and examination of hematology smears

Parasitology

Animal handler

Assist in surgeries

Dental care

X-ray technician

Texas Veterinary Medical Diagnostic Laboratory, College Station, TX 2001-2004

Laboratory Technician

Preparation of cytology slides

Serum chemistries using a Hitachi 911 serum chemistry analyzer

oCanine

oFeline

oEquine

oBovine

oAvian

oPrimate

Cell differentials

Parasitology

Urinalysis

Complete Blood Cell Counts

oCanine

oFeline

oEquine

oBovine

oCaprine

oAvian

oReptilian

PUBLICATIONS

Smith, K.L., Li, Y., Henney, P. J., Sells, S., Pronost, S., Lu, Z., Crossley, B. M., Cook, R. F., and Balasuriya, U. B. R. 2012. New Real-Time PCR Assay using Allelic Discrimination for Detection and Differentiation of Equine Herpesvirus-1 strains with A2254 and G2254 Polymorphisms. JCM, June 2012, Vol. 50:1981-1988.

Smith, K. L., Allen, G. P., Branscum, A. J., Cook, R. F., Vickers, M. L., Timoney, P. J., and Balasuriya, U. B. R. 2010. The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions. Vet Microbiol 141:5-11.

CONFERENCE PRESENTATIONS

Smith, K. L., Li, Y., Breheny, P., Cook, R. F., Henney, P. J., Sells, S., Pronost, S., Lu, Z., Crossley, B. M., Timoney, P. J., and Balasuriya, U. B. R. 2012. A new allelic discrimination real-time PCR assay for detection and differentiation of A2254 and G2254 strains of Equine Herpesvirus-1. International Equine Infectious Disease Conference, October 21th - 26th, 2012, Lexington, KY.

Smith, K. L., Li, Y., Breheny, P., Cook, R. F., Henney, P. J., Sells, S., Pronost, S., Lu, Z., Crossley, B. M., Timoney, P. J., and Balasuriya, U. B. R. 2012. A new allelic discrimination real-time PCR assay for detection and differentiation of A2254 and G2254 strains of Equine Herpesvirus-1. American Association of Veterinary Laboratory Diagnosticians, October 18th - 24th, 2012, Greensboro, NC.

Smith, K. L., Allen, G. P., Branscum, A. J., Cook, R. F., Vickers, M. L., Timoney, P. J., and Balasuriya, U. B. R. 2009. The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions. American Association of Veterinary Laboratory Diagnosticians, October 7th - 14th, 2009, San Diego, CA.

Smith, K. L., Allen, G. P. 2007. Tracing the origin of the recent rise in neuropathogenic EHV-1. Conference of Research Workers in Animal Diseases, December 2nd-4th, 2007, Chicago, Ill.

GRANT PROPOSALS/PROTOCOLS

IACUC Protocol: “The Effects of Serial Cell Culture Passage on Virulence and Pathogenesis of the T953 strain of EHV-1”, April 2011

Grant Proposal: “Molecular Characterization of Neurovirulent EHV-1 Strains”, Grayson-Jockey Club Research Foundation, April 2011

Grant Proposal: “Development of a Genetically Defined Live Attenuated Equine Herpesvirus-1 Vaccine”, Pfizer Animal Health, Oct. 2010

REFERENCES

Dr. Richard B. Jones, PhD

Beckman Coulter

481 California St

Grants Pass, OR 97526 USA

Phone: 541-***-****

Mobile: 773-***-****

Email: *************@*****.***