WILSON A. POSSO RAMOS, M.S.

*** * ******* **., ******, NJ 07036, 973-***-****, acyz5d@r.postjobfree.com

OBJECTIVE

To work in a state of the art laboratory that employs new analytical methods currently practiced in the Pharma industry.

EXPERIENCE

4/2008 TO PRESENT

ZEUS Scientific, Inc.

Branchburg, NJ

ELISA ANTIGEN OPTIMIZATION (APRIL 2008 TO PRESENT)

ELISA Antigen Optimization is the single most important process during the manufacturing of ELISA products. Responsibilities include optimization of all antigens received prior to the manufacturing of ELISA products. Some antigens require processing prior to optimization:

1.Calf thymus DNA purification using mung bean nuclease

2.Mycoplasma processing using a PBS dialysis buffer and CHAPS as a detergent

3.Toxoplasma processing using DOC buffer

4.Verifying Lyme Antigen protein using SDS-PAGE analysis

Research and Development of new ELISA products which include T. pallidum, HSV 1 gG IgG, as well as HSV 2 gG IgG. Creation of proper coating and blocking buffers were established as well as types of plates used. Validating the optimal bioconjugate titrations for the manufacturing of new products. Creation of panels with ranges from negative, equivocal, and positives. Analysis of samples performed using spectroscopy, Immuno Flourecent Assay, and bead conjugation. Submit validation documents to FDA for approval for the manufacturing of new diagnostic products.

Current research includes the validation of Mycoplasma ELISA plate coating using a new novel method that would eliminate the need of processing antigen in PBS buffer.

QUALITY ASSURANCE (JULY 2012 TO PRESENT)

Quality Assurance responsibilities include Batch Record Review, assuring FDA and ISO guidelines are followed during the manufacturing of various diagnostic products. Review paperwork submitted during each step of manufacturing for completeness, accuracy in calculations, and to ensure that SOP was followed. Update SOPs and develop new SOPs to reflect quality standards practiced during the manufacturing of current and future products.

EDUCATION

2009-2014

Kean University

Union, NJ

M.S. IN BIOTECHNOLOGY

Research thesis on the separation of HSV 1 and HSV 2 Antigens in glycine buffer. Methods used in the validation include Isocratic separation with Flexar Analytical HPLC and a C18 column. Detection of samples were obtained using Thermo Fischer Orbitrap HPLC-MS.

2003-2008

Rutgers University

New Brunswick, NJ

B.A. IN GENETICS/MICROBIOLOGY

Research in the development of purifying anti-GFP IgY from chicken eggs using Three Phase Partitioning. Optimization included percentage of T-butyl added to egg yolk prior to centrifugation. Using Sorval centrifugation, separation was achieved based on relative density of antibody comparable to T-butyl.

Discovery of VAB-10 gene in C. remanei using RNAi as a gene knockout method. Gene was then amplified employing RT-PCR method and results published to Pubmed.

2000-2003 Essex County College Newark, NJ

A.S. IN BIOLOGY/PRE-MED

LANGUAGES

Fluent in English, Spanish, and Portuguese.

REFERENCES

References are available on request.

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