Sarij Abulangea

**** ******* *****, **, ********* B

Washington, DC 20001

202-***-****

acyvho@r.postjobfree.com

OBJECTIVE: My objective is to obtain a dynamic, challenging opportunity that contributes to the outstanding success of an organization via my more than 13 years of training and experience. I am seeking a position within an organization where prior experience, personal ability, and a commitment to professionalism would be of value.

EDUCATION

Bachelors of Science, Biology, minor in Chemistry, Howard University 2012

Member of the Science Club and African Student Association

WORK EXPERIENCE

Catholic Charities Washington, DC

Program Assistant 01/04/2016 – 04/15/2016

•Perform the intake process for all clients and provide appropriate referrals.

•Provide direction and crisis intervention to clients at the facility on an as needed basis.

•Maintain the cleanliness, safety, and security of the facility.

•Enforce established rules and regulations, and mediate and resolve conflicts among residents.

Make log entries for assigned shift.

•Inform Supervisor and all appropriate staff of house concerns requiring resolutions or follow-up.

•Participate in the quality improvement (CQI) process to deliver best practice services.

•Participate in agency and program specific trainings.

•Enhance the client environment by creating a hospitable and customer-oriented facility.

•Monitor clients’ self-administration of medication, as needed.

George Washington University Hospital Washington, DC

Volunteer Patient Experience 9/2014 – Present

Assist Intensive Care Unit and Emergency Department by serving coffee, refreshments, and delivering flowers to patients

Assist Pathology Department by collecting, registering, and delivering specimen at appropriate locations. Specimen Accessioning and processing. ( Laboratory receiving) is the section of the laboratories where specimens are received, sorted, entered into the laboratory information System labelled with barcoded labels and processed. Processing a specimen may include mixing the specimen to ensure that all the components are evenly distributed throughout the sample or spinning the specimen in a centrifuge to separate the serum/ plasma layer from the red cells. Once the processing step has been completed, the specimens are forwarded on to the various divisions of pathology and laboratory Medicine for testing.

Perform administrative duties including answering phones and assisting nurses with various tasks

Stock and take inventory of medical supplies

Dedicated volunteer working 5 days a week to gain experience in the field

Howard University Washington, DC

Office Assistant, Office of the Dean, College of Arts and Sciences 10/2008 – 5/2012

Research Assistant, Department of Biology 10/2008 – 5/2012

Laboratory Assistant, Department of Pathology 8/2009 – 11/2010

Assisted Dean and supported staff by performing administrative duties including organizing the office, filing and copying paper work

Greeted and assisted visitors

Supported payroll activities and performed data entry

Answer and direct phone calls

Organized and scheduled meetings and appointments

Maintained contact lists

Produced and distributed correspondence memos, letters, faxes and forms

Assisted in the preparation of regularly scheduled reports

Developed and maintain a filing system

Research Assistant: PCR: reaction goes through 3 steps.1- Strand Separation or denation, the two strands of the parent DNA molecule are separated by heating the solution to 95 degree for 15 seconds. 2- Hybridization or annealing of two oligounclaotide primers ( one is a reverse primer and the other is a forward primer).The solution is quickly coded to 54 degree to the primer anneal to a DNA strand. One primer anneal to the 3 end of the target ( template strand) While the other primer anneals to the end of complementary target strand. Then each copy will be the template in the next cycle. This primer annealing also depends on the melting temp (Tm) of the primer.3- DNA synthesis or elongation or extension. The solution is then heated to 72 degree the optimal temperature for Tag DNA polymerase. This is a polymerase from a thermophilic bacterium, thermos aguaticus, which liver in hot springs. The polymerase elongates both primers in the direction of the target sequence because DNA synthesis is in 5to 3 direction. DNA synthesis continues on both strands and continues beyond the target sequence ( Berg et al .149-150).All 3 steps above are considered as one cycle; it takes about 25-40 cycles in order to amplify DNA template. The number of cycles depends on the amount of DNA available and how much PCR product you want to yield. Cell Culture: DMEM, Dulbecco,s modified Eagle Medium, DMEM/F12, Dulbecco,s, Modified Eagle medium: Nutrient mixture F-12, F-10 Nutrient mixture. Ham;s F-12 Nutrient mixture, and many more. It depends on Gibco products that meet all of your media needs.

Running gel DNA: 1-Break open ( lyse) .the cells or virus containing the DNA of interest. This is often done by sonicating or bead beating the sample vortexing with phenol (sometimes heated is often effective for breaking down protienacious cellular walls or viral capsids. The addition of a detergent such as SDS is often necessary to remove lipid membranes.2- DNA associated proteins, as well as other cellular proteins, may be degraded with the addition of a protease. Precipitation of the protein is aided by the addition of a salt such as ammonium or sodium acetate.When the sample is vortexed with phenol-chloroform and centrifuged the proteins will remains in the organic phase and can be drawn off carefully. The DNA will be found at the interface between the two phases.3- DNA is the precipitated by mixing with cold ethanol or isopropanol and then centrifuging. The DNA is insoluble in the alcohol and will come out of solution, and the alcohol serves as a wash to remove salt previously added.4- Wash the resultant DNA pellet with cold alcohol again centrifuge for retrieval of the pellet.5- After pouring the alcohol off the pellet and drying, the DNA can be re- suspended in a buffer such as tris or TE.6- Presence of DNA can be confirmed by electrophonresing on an agarose gel containing ethidium bromide, or another fluorescent dye that reacts with the DNA, and checking under UV light.

•Assisted professor with chemical solution preparation and research

•Collected and processed specimens and performed data analyses

•Labelled and refrigerated lab work to ensure the specimen was not contaminated

•Adhered to safety and cleaning standards

•Organized specimens and filed medical records

•Adhered to safety and cleaning standards

Day and Zimmerman Lansdale, PA

Security Officer (Merck Contractor) 5/2003 – 8/2006

Secured front lobby by monitoring and authorizing entrances and departures of both employees and visitors

Monitored surveillance cameras of parking lots and internal cameras

High level of attention to detail to ensure security of building premises

Wrote hourly reports of activities, irregularities, and reported incidents

Maintained constant communication with command center

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