MARIA LVOVA

Cell Phone: 609-***-****

E-mail: acyura@r.postjobfree.com

RESUME

M.D., experienced molecular biologist with extensive hands-on background in

Next Generation Sequencing (DNA/cfDNA and RNA, human blood and tissues,

FFPE, animal tissues, mammalian cells, amplicon and capture based, full

workflow with data analysis), NGS assay development, assay transfer and

validation, laboratory set up and operation. CLIA laboratory experience.

Lab Management experience.

EMPLOYMENT AND EXPERIENCE

Senior Scientist, MDx

January 2017 - current

Scientist, Molecular Diagnostics

January 2016 - January 2017

KEW Inc., Cambridge, MA

. Lead Scientist with Research and Development Team.

NGS Assay Development for Illumina Platform. Hybrid Capture based

DNA Sequencing. Duplex tag sequencing for Liquid Biopsy

applications. Molecular barcoding. Currently leading Liquid Biopsy

product development.

. Assay Optimization. Maximizing DNA yield from low quality FFPE

samples. Frag Library rescue protocols development for low

yield/quality samples. Capture baits design and performance

optimization. MiSeq vs NextSeq performance concordance. Paired end

vs Single end sequencing. Lab automation.

. Assay Transfer. Providing training and day to day guidance for Assay

Transfer Department. SOP and R&D reports.

. Assay Validation. Planning validations for CLIA team. Creating

validation sets for wet lab and bioinformatics team.

. CLIA operations support. Assisting CLIA personnel in troubleshooting

on day to day basis.

. Training and mentorship for R&D group members.

. Reporting to VP, Research and Technology.

Scientist I/LabManager

August 2015 - January 2016

Research Associate/Lab Manager July 2010 -

August 2015

Children's Hospital of Philadelphia,

Center for Mitochondrial and Epigenomic Medicine (CMEM), Philadelphia, PA

. Established Next Generation Sequencing program at the Center and

served as a reference scientist for mitochondrial DNA sequencing and

analysis in both clinical and population samples.

. Successfully developed, optimized, validated and executed NGS based

assays for mitochondrial and nuclear DNA, RNA.

. Consulted curators of MITOMAP/MITOMASTER database and portal for

mtDNA and nuclear-encoded mitochondrial genes variations.

. Key personnel on NIH grants and pharmaceutical companies' contracts.

. Trained and mentored laboratory staff members and graduate students.

. Lab Manager. Lab setup from the scratch, vendor negotiation,

ordering, equipment support and maintenance.

. Institutional Biosafety Committee protocol writing and compliance

assurance. Research safety compliance liaison for the Center. Study

coordinator for multiple IRB protocols. SOP writing.

Junior Specialist

Apr. 2006 - June 2010

University of California,

Center for Molecular and Mitochondrial Medicine and Genetics (MAMMAG),

Irvine, CA

. Conducted scientific research in human mitochondrial genetics, genetic

variation, human population history, evolution, aging, and mitochondrial

medicine with a primarily focus on the role of mtDNA variation in

metabolic disorders (such as Type II Diabetes Mellitus and Metabolic

Syndrome).

. Created and maintained variety of human cell lines (primary,

immortalized, cybrids). Characterized physiology of created cell lines

and assessed their mitochondrial function using advanced cell biology

techniques.

. Worked in CLIA-certified/reference lab, part of the Center, on

developing and optimization of clinical diagnostic SOPs for DNA

analysis for the patients with suspected mitochondrial disease;

organized workflow for research part of IRB study, performed molecular

tests optimization and validation; consulted on mitochondrial

haplotyping and interpretation of genetic analysis results.

. Developed, tested, evaluated, and troubleshot new protocols and methods

of molecular analysis.

. Consulted/trained other researchers, visiting scholars, and students.

Researcher

2004-2005

University of California,

Center for Molecular and Mitochondrial Medicine and Genetics (MAMMAG),

Irvine, CA

. conducted research on mitochondrial DNA samples of Native Siberians

(Eskimo, Chukchi, Yukagirs, Nganasans etc.).

TECHNIQUES AND SKILLS

Molecular biology. Extensive experience with DNA extraction, purification,

ligation, cloning, transformation, PCR and qRT-PCR, electrophoresis, RFLP,

SNaPshot, CE sequencing on 3500 and 3130xl ABI Genetic Analyzers, High

throughput Next Generation Sequencing (NGS) on Ion Torrent PGM/Ion Proton

and Illumina MiSeq/NextSeq (full workflow from DNA/RNA purification to

sequencing and data analysis), genomic libraries preparation for NGS. ChIP-

Seq, RNA-Seq, Whole Exome Sequencing, mtDNA Sequencing. Agilent

Bioanalyzer, Pippin Prep, Liquid robotic handlers (operation and

programming). Expert in working with DNA samples from human body fluids and

tissues, FFPE.

Cell culture. Large scale maintenance of human cell lines (lymphoblasts,

primary fibroblasts, osteosarcoma, mitochondria-deprived cells, hybrid

cells) under aseptic and antibiotic-free conditions. EBV mediated

transformation of human lymphocytes. Enucleation, mitochondria extraction,

cell fusion; maintaining cells under selective condition.

Cell biology. Immunohistochemistry, FACS, mitochondrial physiology

(mitochondrial respiratory chain enzymatic complexes assays, oxygen

consumption, ROS production, membrane potential measurements, in-vivo

labeling and analysis of mitochondrial translation products). Fluorescent

and confocal microscopy.

Microbiological and protein analysis. E.coli maintenance, protein

extraction and purification, western-blotting, protein electrophoresis.

Computers/Software. Advanced level. PC and Apple; MSOffice (Word, Excel,

PowerPoint etc.), Adobe Acrobat; Sequencher, GeneMapper, IVG, Galaxy,

Partek, DNAStar, UCSC Genome Bioinformatics; ANNOVAR, NCBI databases and

tools; MITOMAP; electronic data management; Electronic Laboratory

Notebooks, LIMS.

EDUCATION AND DEGREES

2012 NGS-230 Ion Personal Genome Machine Systems Operational Training

Life Technologies University

2003 License of Family Care Physician. Novosibirsk Regional Board of

Internal Medicine, Novosibirsk, Russia.

2002 Medical Diploma (Magna cum laude). Novosibirsk State Medical

University, Novosibirsk, Russia.

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