Plant Medical

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MARYAM SANEI, Ph.D.

Department of Botany and Plant Science, University of California, Riverside, CA 92521 Phone: 951-***-****; Email: *******@***.***

OBJECTIVE: Seeking full-time position as Research and Development (R&D) Scientist in Biotechnology. Professional Experiences

University of California-Riverside, Department of Genomics January 2013- Present Howard Hughes Medical Institute (HHMI)/National Institutes of Health (NIH) Postdoctoral Scholar: Molecular Biology & Genetics

• Identification of the proteins involve in tailed micro-RNA turnover using In Vivo and In Vitro assays.

• In Vivo assays including: Investigation of miRNA tailing status in natural variants and engineered mutants of candidate genes generated by artificial miRNA and CRISPR.

• In Vitro assays including: Expression and Purification of recombinant proteins, Enzymatic assays, and Gel shift assays.

University of California-Davis, Department of Life Sciences April 2012-December 2012 Howard Hughes Medical Institute (HHMI) Postdoctoral Scholar: Molecular Biology & Plant Breeding

• Increase the efficiency of haploid formation in tail-swap Arabidopsis lines through decreasing the chromosome number.

• Generating Arabidopsis with fused chromosomes by two approaches: 1- Non- homologous recombination end joining induced by Cre/Lox system. 2- Non-homologous chromosome end joining after double strand DNA breaks close to telomeres using TALEN system.

Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Department of Chromosome Structure and Function, Gatersleben, Germany October 2007-June 2011 German Research Foundation (DFG) Fellow in Leibniz Institute of Plant Genetics and Crop Plant Research: Molecular Cytogenetics & Plant Breeding

• Investigating the mechanism underlying selective elimination of the paternal chromosomes during the early development of barley Hordeum bulbosum crosses.

• Results of the study established that loss of centromeric protein (CENH3) triggers inactivation of Bulbosum centromere and eventually eliminates Bulbosum genome. Agricultural Biotechnology Research Institute of Iran (ABRII), Department of Cytogenetics Research Assistant: Cytogenetics & Plant Breeding May 2004-May 2006

• Created and characterized a bank germplasm of Crocus species through comparatively studying allies from different regions and cultivated saffron.

• The karyotype of Crocus species was characterized with classical chromosome staining and C-banding.

Technical Qualifications

Molecular and biochemical Techniques

Proficient in a variety of classical and cutting edge molecular biology techniques including:

• DNA, RNA, Southern and Northern blots, RT-PCR, Race PCR and Real-Time PCR, RNA extraction from a small number of cells, Small RNA library construction, Cell free 2

transcription and translation.

• Skilled in plant transformation, Gene cloning, Genetic engineering using TALEN and CRISPR techniques, Artificial microRNA, and Cre/Lox systems design.

• Biochemical expertise in Protein expression and Protein purification, FPLC (Gel filtration chromatography, Ion-exchange chromatography, Affinity chromatography), Recombinant protein, Site-directed mutagenesis, In Vitro enzymatic assay, Immunoprecipitation, Antibody generation and evaluation.

Bioinformatics techniques

• Sequence Alignment and BLAST using ClustalW, Lasergene, and Geneios.

• Artificial miRNA design (WMD3) and Primer design.

• Familiar with DNA, Protein and miRNA Sequence Databases (NCBI, TAIR, EMBL-EBI, BAR, and miRBase).

Cell Biology Techniques

• Adept in cell culture preparation and maintenance, Flow cytometry technique and data analysis (Cell sorting, Cell cycle, and Ploidy level analysis), Developmental study, Plant hybridization including Arabidopsis and Barley species. Cytogenetic Techniques

• Mitotic and Meiotic chromosome preparation by squash, cell suspension, and cell drop techniques.

• Mastery at FISH, GISH, and Fiber FISH techniques, Immunostaining and Fiber immunostaining, EdU Banding, and C-banding.

• Experienced in Optical microscopy, Fluorescent microscopy, and Confocal microscopy.

• Microscopic image analysis, Fluorescence image quantification methods using NIH ImageJ, Photoshop, AnalySIS and TINA.

Education

Ph.D, Plant Breeding, Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)/ Martin-Luther University, Halle, Germany. June 2011 M.Sc, Plant Breeding, Mazandaran University May 2006 B.Sc, Agronomy and Plant Breeding, Bahonar University June 2002 Peer reviewed publications

1- Sanei, M., Chen, X. (2015) Mechanisms of microRNA turnover. Current Opinions in Plant Biology, 27:199–206.

2- Schroeder-Reiter, E., Sanei, M., Houben, A and Wanner, G. (2012). Current SEM techniques for de- and re-construction of centromeres to determine 3D CENH3 distribution in barley mitotic chromosomes. Journal of Microscopy, 246: 96–106.

3- Sanei, M., Pickering, R., Kumke, K., Nasuda, S and Houben, A. (2011). Loss of CENH3 from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids. Proceedings of the National Academy of Sciences, 108 (33): 498-505. Cited more than 110 times. 4- Sanei, M., R. Pickering, J. Fuchs, A.M. Banaei Moghaddam, A. Dziurlikowska and Houben, A. (2010). Interspecific hybrids of Hordeum marinum ssp. marinum x H. bulbosum are mitotically stable and reveal no gross alterations in chromatin properties. Cytogenetic and Genome Research. 129: 110-116. 5- Houben, A., Sanei, M and Pickering, R. (2010). Barley doubled-haploid production by uniparental chromosome elimination. Plant Cell Tissue Organ Culture. 104:321–327. 6- Sanei, M., Agayev, Y., Rahimian, H and Sohelivand, S. (2007). New cytotype of Crocus pallasii from the west of Iran. Acta Horticulture. 739:107-112.

Residency status in United States: Permanent Resident (Green Card holder).

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