ZHENG WU

CONTACT INFORMATION

Phone: 281-***-**** (cell)

Email: acys03@r.postjobfree.com

EDUCATION

Ph.D. in Chemistry, GPA 3.87/4.0, University of Houston, Houston, Texas; 1992.

M.S. in Chemistry, GPA 4.0/4.0, Southern Illinois University, Carbondale, IL; 1988.

Categorical Certification Degree in Clinical Chemistry, GPA 4.0/4.0, School of Allied Health Sciences, University of Texas Medical Branch at Galveston, Texas; 2007.

PROFESSIONAL QUALIFICATION

Certified Medical Technologist in Chemistry (ASCP); 2007.

WORKING EXPERIENCES

2002 - Present Title: Assistant Manager/Research Scientist II

Employer: Biomolecular Research Facility proteomics core laboratory, Department of Biochemistry and Molecular Biology,

University of Texas Medical Branch, Galveston, Texas.

Job duties: Established the proteomics core laboratory according to the principle investigator’s directives. Develop HPLC methods for analysis and separation of proteins, peptides and biological mixtures. Conduct 2D gel electrophoresis and related methodologies, and operate proteomic robotics for downstream protein identifications by MALDI-TOF and data base search. Develop CE methods for analysis of clinical serum or plasma samples in the study of clinical proteomics. Advise, collaborate, and train on/off campus researchers in running and imaging 1D and 2D gels with different instruments. Maintain instruments and handle billings for the lab.

1995 - 2002 Title: Research Scientist

Employer: Introgen Therapeutics, Inc., Houston, Texas.

Job duties: Developed HPLC methods for analyses of the production, purification, and formulation of the adenovirus encoding the human p53 gene (Adp53). Characterized Adp53 in aqueous and lyophilized formulations with HPLC, bioactivity assay. Developed analytical assays to support studies of non-viral gene delivery, including online monitoring of plasmid DNA production and characterization of liposome/DNA-liposome complexes by HPLC, particle size analysis, gel-electrophoresis, and in vitro/vivo gene uptake studies. Interfaced with QC and QA groups to transfer developed HPLC assays for applications in QC group.

1994 - 1995 Title: Research Scientist

Employer: GeneMedecine, Inc. Woodlands, Texas.

Job Duties: Synthesized and purified hetero-bi-functional cross-linking reagents, small peptide residues and sugar derivatives as targeting ligands for gene delivery. Characterized the reagents and ligands by HPLC, NMR (1H or 13C), FTIR, UV-vis, and mass spectrometry.

1992 - 1994 Title: Senior Research Assistant

Employer: Department of Clinical Investigation, M.D. Anderson Cancer Center, University of Texas, Houston, Texas.

Job Duties: Synthesized and purified a variety of anticancer drugs including their radioactive analogs. Established the identity and chemical structure of the drugs by various spectral techniques including NMR (1H and 13C), UV-vis, mass spectrometry and HPLC. Studied the covalent modification of DNA via the sugar moiety of doxorubicin using HPLC in conjunction with radiochemical methods. Studied the toxicity of the drugs on mice.

1988 - 1992 Title: Research Assistant (Graduate Student)

Employer: Department of Chemistry, University of Houston, Houston, Texas.

Research Projects: Synthesized and purified a series of (ap)4Rh2-R compounds, where ap = 2-anilinopyridinate and R = -(CºC)2Si(CH3)3, -CH3, -CH=CH2, -CºCH and -(CºC)2-Rh2(ap)4. The structures of these compounds were identified by X-ray crystallography and mass spectrometry. The compounds were studied by electrochemistry (cyclic voltammetry), ESR, FTIR, and UV-vis spectroscopy.

1986 - 1988 Title: Research Assistant (Graduate Student)

Employer: Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois.

Research Project: Investigated the reaction of Re2Cl4(PEt3)4 with ethyl chloride and hydrogen chloride under different conditions to establish a mechanism for the formation of [PEt4][Re2Cl6(PEt3)2H].

AWARDS RECEIVED

● Merit employee (1992-1993) in M.D. Anderson Cancer Center, University of Texas, Houston, Texas.

● Welch Foundation Scholarship, in the Chemistry Department, University of Houston (1991 to 1992).

LEADERSHIP AND SERVICES

● Chemical Safety Officer, Introgen Therapeutics, Inc., Houston, Texas, 1996-2002.

● Chemical Safety Officer, Department of BRF, UTMB, 2003-present.

PATENTS

● Zhang, S., Thwin, C., Wu, Z., and Cho, T., “An Improved Method for the Production and Purification of Adenoviral Vectors”, Filed on November 20, 1997, File # INRP: 058.

● Wu, Z., and Zhang, S. “Formulations of Adenovirus for Gene Therapy”, Filed on November 16, 1999, File # INRP:067.

SELECTED PUBLICATIONS & PRESENTATIONS

● Kizhake V. Soman, Susan J. Stafford, Konrad Pazdrak, Zheng Wu, Xuemei Luo, Wendy I. White, John E. Wiktorowicz, William J. Calhoun, Alexander Kurosky,

Comparative Activation of Human Peripheral Blood Eosinophils by Cytokines in a Time-Course Proteomic/Phosphoproteomic Study

Target journal: Journal of Proteome Research, to be submitted

● Kizhake Soman; Susan Stafford; Konrad Pazdrak; Zheng Wu; Xuemei Luo; Wendy White; John Wiktorowicz; William Calhoun; Alexander Kurosky

“Comparative Proteomics of Time-Course Activation of Eosinophils with Cytokines, Applied Singly and in Pairs, Using Multiple Proteomic Platforms”

US HUPO 2016 Conference (Boston, MA) abstract, submitted on 2/10/2016.

● Brasier AR, Zhao Y, Wiktorowicz JE, Spratt HM, Nascimento EJ, Cordeiro MT, Soman KV, Ju H, Recinos A 3rd, Stafford S, Wu Z, Marques ET Jr, Vasilakis N.

“Molecular classification of outcomes from dengue virus -3 infections”

J Clin Virol. 2015 Mar; 64: 97-106.

● Konrad Pazdrak, Paneez Khoury, Kizhake Soman, Nicole Holland-Thomas, Michelle Makiya, Christof Straub, Zheng Wu, Amy Klion, Alexander Kurosky.

“Differential Proteomic Analysis of Eosinophils from Patients with Glucocorticoid Responsive or Resistant Hypereosinophilic Syndrome”

Poster presentation at Annual Meeting of American Academy of Allergy, Asthma and Immunology, 2014 San Diego, CA

● Kizhake Soman; Farrah Kheradmand; John Wiktorowicz; Zheng Wu; Anthony Haag; Susan Stafford; Robert English; Alexander Kurosky

“Plasma Biomarker Discovery for COPD by 2-D Gel Electrophoresis and Mass Spectrometry”

Poster presentation at 9th Annual US HUPO Conference 2013, Baltimore MD

● Armaiz-Pena GN, Allen JK, Cruz A, Stone RL, Nick AM, Lin YG, Han LY, Mangala LS, Villares GJ, Vivas-Mejia P, Rodriguez-Aguayo C, Nagaraja AS, Gharpure KM, Wu Z, English RD, Soman KV, Shahzad MM, Zigler M, Deavers MT, Zien A, Soldatos TG, Jackson DB, Wiktorowicz JE, Torres-Lugo M, Young T, De Geest K, Gallick GE, Bar-Eli M, Lopez-Berestein G, Cole SW, Lopez GE, Lutgendorf SK, Sood AK.

“Src activation by β-adrenoreceptors is a key switch for tumour metastasis”

Nat Commun. 2013; 4:1403.

● Wiktorowicz J E, English R D, Wu Z, Kurosky A.

“Model studies on iTRAQ modification of peptides: sequence-dependent reaction specificity”

J Proteome Res. 2012 Mar 2; 11(3):1512-20.

● Brasier AR, Garcia J, Wiktorowicz JE, Spratt HM, Comach G, Ju H, Recinos A 3rd, Soman K, Forshey BM, Halsey ES, Blair PJ, Rocha C, Bazan I, Victor SS, Wu Z, Stafford S, Watts D, Morrison AC, Scott TW, Kochel TJ; Venezuelan Dengue Fever Working Group.

“Discovery proteomics and nonparametric modeling pipeline in the development of a candidate biomarker panel for dengue hemorrhagic fever”

Clin Transl Sci. 2012 Feb; 5(1): 8-20.

● C. Straub, B. Xu, Z. Wu, T. Wood, A. Haag, K. Pazdrad, A. Kurosky

Peripheral Blood Eosinophils Are Not Activated By Highly Purified Recombinant High Mobility Group Box 1 (HMGB1) Protein”

Poster presentation at Annual Meeting of American Academy of Allergy, Asthma & Immunology 2012, Orlando, FL

● A. Kurosky, C. Straub, K. Pazdrak, T. W. Young, S. J. Stafford, Z. Wu, J. E. Wiktorowicz, A. M. Haag, R. D. English, K. V. Soman;

“Proteome Analysis of the Peripheral Blood Human Eosinophil”

Poster presentation at Annual Meeting of American Academy of Asthma, Allergy and Immunology, 2009, Washington DC.

● Straub C, Pazdrak K, Young TW, Stafford SJ, Wu Z, Wiktorowicz JE, Haag AM, English RD, Soman KV, Kurosky A.

“Toward the Proteome of the Human Peripheral Blood Eosinophil”

Proteomics Clin. Appl. 2009; 3(10):1151-1173.

● K. Pazdrak, C. Straub, Z Wu, A. Kurosky

“Profiling of Phosphoproteins in GM-CSF-activated Eosinophils Reveals Lineage-Specific Signaling Molecules”

Poster presentation at Annual Meeting of American Academy of Asthma, Allergy and Immunology, 2008 Philadelphia

● Forbus J, Spratt H, Wiktorowicz J, Wu Z, Boldogh I, Denner L, Kurosky A, Brasier RC, Luxon B, Brasier AR.

“Functional analysis of the nuclear proteome of human A549 alveolar epithelial cells by HPLC-high resolution 2-D gel electrophoresis”

Proteomics 2006 May; 6(9): 2656-72.

● Zheng Wu, John E. Wiktorowicz, Robert English, and Alexander Kurosky

“Evaluation of iTRAQ Reagent Using Different Peptides”

Poster presentation at Proteomics Investigator Meeting, North Bethesda, Maryland, September 2005.

● S. Joe Haidacher, Ronald G. Tilton, Zheng Wu, Alexander Kurosky, Allan R. Brasier, and Larry Denner

“Increasing the depth of interrogation of the proteome and phosphor-proteome using HPLC pre- fractionation of intact proteins prior to 2D gel electrophoresis to study the pathogenesis of diabetes”

Poster presentation at ASMS Meeting, 2005, San Antonio, Texas, June, 2005.

● Yashoda M. Hosakote, Antonieta Guerrero-Plata, Zheng Wu, Anthony Haag, Heidi Spratt, Susan J. Stafford, Shawn Castro, Allan R. Brasier, Alexander Kurosky, and Roberto P. Garofalo

“Newly identified proteins in the lower respiratory tract by high resolution 2D-gel electrophoresis and peptide mass fingerprinting”

Poster presentation at Proteomics Investigator Meeting, North Bethesda, Maryland, Nov. 2004.

● Allan R. Brasier, Heidi Spratt, Zheng Wu, Istvan Boldogh, Yuhong Zhang, Roberto P. Garofalo, Antonella Casola, Jawad Pashmi, Anthony Haag, Bruce Luxon and Alexander Kurosky

“Nuclear Heat Shock Response and Novel Nuclear Domain 10 reorganization in Respiratory Syncytial Virus-Infected A549 Cells Identified by High Resolution 2D Gel Electrophoresis”

Journal of Virology, 78 (2004) 11461.

● Yafei Huang, Alexander Kurosky, Zheng Wu, Anthony Haag and Lee-Jane W Lu,

“Proteomic analysis of nipple aspiration fluid in non-lactating pre-menopausal women by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)”

Presented at the 2003 annual meeting of the Endocrine Society, Abstract # 85027.

● Ramesh, R., Saeki, T., Templeton, N S, Wu, Z., Wilson, D R, etc., and Roth J A.

“Successful Treatment of Primary and Disseminated Human Lung Cancers by Systemic Delivery of Tumor Suppressor Genes Using an Improved Liposome Vector”

Molecular Therapy, 3 (2001) 337.

● John L. Bear, B. Han, Z. Wu, E. Van Caemelbecke and Karl M. Kadish,

“Synthesis, Electrochemistry and Spectroscopic Characterization of Bis-Dirhodium Complexes Linked by Axial Ligands”

Inorg. Chem, 40 (2001) 2282.

● Ramesh, R., Saeki, T., Templeton, N S, Wu, Z., Wilson, D R, etc., and Roth J A.

“Inhibition of Primary and Disseminated Human Lung Cancers by Systemic Delivery of p53 Tumor Suppressor Gene Using an Improved Liposome”

Proc. Amer. Assoc, Cancer Res., 41 (2000) 603.

● Alspaugh, E., Roth, J A, Wu, Z., etc., and Templeton, N S.

“Non-Viral Vectors for the Treatment of Disease”

Poster Presentation in Keystone Symposia on Molecular and Cellular Biology of Gene Therapy, at Salt Lake City, Utah, Jan. 1999

● Wu, Z. and Zhang, S.

“Development of Lyophilization Formulations and Cycles for Long Term Storage of Adenovirus Encoding with Human p53”

Presentation at Williamsburg BioProcessing Conference at Williamsburg, Virginia, Nov., 1998.

● Wu, Z., Wilson, D. and Zhang, S.

“An HPLC Method for Analysis of the Production and Purification of a Type 5 Recombinant Adenovirus Encoding with Human p53”

Poster Presentation at Williamsburg BioProcessing Conference at Williamsburg, Virginia, Oct. 1996.

● Wu, Z. and Farquhar, D.

“Covalent modification of DNA by the sugar moiety of doxorubicin”

Proc. Amer. Assoc. Cancer Res., 35 (1994) 364.

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