Prasad R. Kopparapu, Ph.D.

**** ** ******* *** *** 282, Corvallis, OR. 97330

Tel: 541-***-**** ? e-mail: acyrb6@r.postjobfree.com

SUMMARY

Toxicologist trained extensively in molecular biology and animal models for discovery of novel therapeutics. My doctoral studies included discovery, characterization and proof-of-concept animal testing of novel small molecules that functionally convert anti-death Bcl-2 protein to pro-death protein inducing cancer cell death. My work also involved discovery of small molecules that act as immune-suppressant by activating Aryl Hydrocarbon receptor (AhR). Initially trained as a Chemical Engineer in my B.S. degree, I bring all the quantitative expertise along with computation skills in solving scientific problems. I have also demonstrated my computer skills while working as clinical programmer at Accenture, India. I am good at managing multiple projects, a team player, adaptable to changes and with excellent communication skills.

EDUCATION

? Ph.D. Toxicology, Oregon State University, September 2016.

? M.S. Environmental Biotechnology (Elective: Molecular Toxicology), JNT University, India, May 2006.

? B.S. Chemical Technology, Loyola Academy, India, Nov 2002.

CORE COMPETENCIES

● Toxicology/Pharmacology

● Drug Discovery & Cancer Therapeutics

● Cancer Biology/ Resistance models

● In vivo Imaging/ Confocal Microscopy

● In vivo Tumor Models Expertise

● Collaborations, Partnerships, & Scientific Writing

● Oncological Pathology & Mechanistic Studies

● Computational Biology

● Small Molecule Screens

● mRNA silencing

● Formulations ● Clinical Research/Data Management

POSITIONS AND EXPERIENCE

? Graduate Research Assistant at Cancer Research Lab, Environmental and Molecular Toxicology, Oregon State University. (Sep 2008-present); Advisor: Dr. Siva Kumar Kolluri

Discovered small molecule functional convertors of Bcl-2 protein that convert them to killers of cancer cells.

Discovered novel small molecules that activate Aryl Hydrocabon receptor (AhR) to induce cell death in cancer cells and those that cause immune suppression by induction of Treg cells.

Performed primary and metastatic models (lung and bone) of human breast cancer in mice.

Experience in developing small molecule formulations for oral and intraperitoneal dosing in animal studies along with toxicity testing.

Well trained in data analysis using command line programming (UNIX), R, analysis of large scale data for RNA-Seq experiments.

Independently developed and tested new hypothesis along with establishing collaborations across departments. Contributed data that led to over $1.8 million in grant funding.

Mentored and trained undergraduate research and new graduate students in molecular biology and animal handling techniques.

My research contributed to 4 peer-reviewed publications so far with 3 more (first author) in preparation and 12 presentations at national and regional conferences.

? Graduate Research Assistant (PhD program) at Environmental Systems Lab, Dept., of Environmental Engineering, Texas A&M University, Kingsville. (Jan 2008 - Jun 2008); Advisor: Dr.Venkatesh Uddameri

Worked on Modeling of nitrogen species in arroyo Colorado river.

Mentored Undergraduate and Masters Student in sample collection and analysis to collect parameters for further modeling.

? Database Designer and Reports Programmer in Global Clinical Discovery team at Accenture Services Ltd., India. (Feb 2007- Nov 2007); Client: Bristol-Myers Squibb

Database designing using Oracle Clinical and reports programing using I-Review (SQL). Good knowledge of clinical data management work flow.

Successfully performed the knowledge transfer from client site and continued to work in team located in 3 different countries and directly reported to the client.

? Masters Thesis at Centre for Environment, JNT University, Hyderabad, India. (Feb 2005 - Mar 2006); Advisor: Dr. Y.Anjaneyulu

Thesis on ?Uptake of heavy metals by the fresh water murrel Channa punctatus in the eutrophic lake Hussainsagar, Hyderabad, India?.

My work involved collecting water, sediment and fish samples from the lake and preparing samples for analysis by ICP spectroscopy for detection of heavy metals.

? Executive-Marketing (Industrial) at Surekha Industrial Marketing Associates (SIMA) Hyderabad, India. (August 2002-July 2003)

My role involved meeting maintenance managers/Leads at pharmaceutical companies in the state and convince them how more expensive synthetic and food grade lubricants (Sentinel Lubricants, USA) can save them money and man hours.

Worked with technicians and client operations team to ensure compliance in product usage and performance.

? Under-graduate summer project at HBL NIFE Power Systems Ltd., India. (Summer 2001). ?Analysis of Carbonates in manufacture of Ni-Cd sintered plate aviation batteries.

SKILL SETS

Wet lab: Designing screening assays for discovery of small molecule targeted therapies, proficient in most molecular methods, generation of cell lines from primary tumors and transgenic lines, In vivo xenograft studies with multiple routes of dosing, breast cancer metastatic models, In vivo bioluminescent imaging, Immuno-histochemistry, confocal microscopy, Flow cytometry, Toxicity testing, Aquatic toxicology, Formulation screening, Particle size analysis, Milling, Microfluidizer, UV/Vis Spectroscopy, Phamacokinetics, HPLC and bioinformatics training in RNA-seq analysis.

Computational Skills: Unix, Python, R, Integrated Review, Oracle Clinical, C programming, SQL, PL/SQL, J-Review, SAS, PRISM.

PUBLICATIONS

Dan Koch, HyoSang Jang, Edmond O?Donnell, Sumit Punj, Prasad Kopparapu, William Bisson, Nancy Kerkvliet, and Siva Kolluri. ?Anti-androgen flutamide suppresses hepatoma cell proliferation via the aryl hydrocarbon receptor mediated induction of transforming growth factor β1. Oncogene 2015 Apr 13. 10.1038/onc.2015.55.

Sumit Punj, Kopparapu PR, Hyo Sang Jang, Jessica L. Phillips, Jamie Pennington, Diana Rohlman, Edmond O'Donnell, Patrick L. Iversen, Siva Kumar Kolluri, Nancy I Kerkvliet. ?Benzimidazoisoquinolines: A new class of AhR ligands that induce AhR-dependent Tregs and prevent murine graft-versus-host disease". PLoS One. 2014 Feb 19;9(2):e88726.

O'Donnell EF, Kopparapu PR, Koch DC, Jang HS, Phillips JL, Tanguay RL, Kerkvliet NI, Kolluri SK. ? the aryl hydrocarbon receptor mediates leflunomide-induced growth inhibition of melanoma cells?. PLoS One. 2012;7(7):e40926

O'Donnell EF, Saili KS, Koch DC, Kopparapu PR, Farrer D, Bisson WH, Mathew LK, Sengupta S, Kerkvliet NI, Tanguay RL, Kolluri SK. ?The anti-inflammatory drug leflunomide is an agonist of the aryl hydrocarbon receptor?. PLoS One. 2010 Oct 1;5(10). pii: e13128.

SCIENTIFIC MEETING/PRESENTATIONS:

Prasad R. Kopparapu, Arnold C. Satterthwait, Xiao-kun Zhang, Siva Kumar Kolluri. ?Bcl-2 Functional Convertors as Breast Cancer Therapeutics?. Poster Presentation at Diet and Optimum Health Conference, Linus Pauling Institute, Sep 2015, OSU.

Invited Talk ?Fighting Cancer with its own tools? at Centre for Genome Research and Biocomputing (CGRB) Fall 2014 conference at Oregon State University Sep 12 2014.

Prasad R. Kopparapu, Edmond F. O'Donnell, Arnold C. Satterthwait, Xiao-kun Zhang, Siva Kumar Kolluri. ?Bcl-2 Functional Convertors as Breast Cancer Therapeutics?. Platform presentation at EMT Research Day, Jan 2014, OSU.

Jessica L. Phillips, Prasad R. Kopparapu, David Castro, Jochen Maurer, Edmond F. O'Donnell, Hyo Sang Jhang, Soheila Kazemi, Nancy Kerkvliet, Robert Oshima, Siva Kumar Kolluri. ?Anti-Cancer Effects of Novel Aryl Hydrocarbon Receptor Ligands in Triple Negative Breast Cancers and Breast Cancer Stem Cells?. Poster presentation at EMT Research Day, Jan 2014, OSU.

Prasad R. Kopparapu, Edmond F. O'Donnell, Arnold C. Satterthwait, Xiao-kun Zhang, Siva Kumar Kolluri. ?Bcl-2 Functional Convertors as Breast Cancer Therapeutics?. Poster Presentation at Cell Death Meeting, Oct 2013, Cold Spring Harbor Labs, NY.

Prasad R. Kopparapu, Edmond F. O'Donnell, Arnold C. Satterthwait, Xiao-kun Zhang, Siva Kumar Kolluri. ?Bcl-2 Functional Convertors as Breast Cancer Therapeutics?. Poster presentation at CGRB Fall 2013 Conference, OSU.

Jessica L. Phillips, Prasad R. Kopparapu, Sumit Punj, Hyo Sang Jhang, Diana Rohlman, Dan Koch, Edmond F. O'Donnell, Nancy Kerkvliet, Siva Kumar Kolluri. ?Characterization of novel AhR ligands and evaluation of their effects in cancer and auto-immune disorders?. Poster presentation at CGRB Fall 2013 Conference, OSU.

Jessica L. Phillips, Prasad R. Kopparapu, Sumit Punj, Hyo Sang Jhang, Diana Rohlman, Dan Koch, Edmond F. O'Donnell, Nancy Kerkvliet, Siva Kumar Kolluri. ?Characterization of novel AhR ligands and evaluation of their effects in cancer and auto-immune disorders?. Poster presentation at EMT Research Day, Jan 2013, OSU

Prasad R. Kopparapu, Edmond F. O'Donnell, Arnold C. Satterthwait, Xiao-kun Zhang, Siva Kumar Kolluri. ?Bcl-2 Functional Convertors as Breast Cancer Therapeutics?. Poster award at Annual CGRB Conference at OSU, Fall 2011.

Prasad R. Kopparapu, Edmond F. O'Donnell, Arnold C. Satterthwait, Xiao-kun Zhang, Siva Kumar Kolluri. ?Bcl-2 Functional Convertors as Breast Cancer Therapeutics?. Poster presentation at Era of Hope Department of Defense Breast Cancer Research Program Conference, Aug 2011.

Prasad R. Kopparapu, Edmond F. O'Donnell, Arnold C. Satterthwait, Xiao-kun Zhang, Siva Kumar Kolluri. ?Bcl-2 as a Target for Breast Cancer Treatment?. Poster presentation at Department of Environmental and Molecular Toxicology Research Day, Oregon State University, Jan 2011.

Prasad R. Kopparapu. ?Bcl-2 as a Target for Breast Cancer Treatment?. Platform presentation at Pacific Northwest Association of Toxicologist (PANWAT) Conference, Oct 2010.

Edmond F O'Donnell, Prasad Kopparapu, Diana Rohlman, Dan Koch, Jang, Hyo Sang, Siva K Kolluri, Nancy I Kerkvliet. ?AHR-mediated Immuno-suppression as a Strategy for Treatment of Autoimmune Disease?. Nuclear Receptors and Disease Meeting, Cold Spring Harbor Laboratory, Sep 2010.

REFERENCES:

Siva Kumar Kolluri, Associate Professor of Cancer Research, Department of Environmental and MolecularToxicology, Oregon State University, acyrb6@r.postjobfree.com, 541-***-**** (PhD Advisor)

Nancy Kerkvliet, Professor of Immunology, Department of Environmental and Molecular Toxicology, Oregon State University, acyrb6@r.postjobfree.com, 541-***-**** (PhD Committee member)

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