Project Research

Successfully lead a team and coordinated with several NIH project to accomplish Basic Science

Research in Cell and molecular biology for more than 15 years. I have experienced and contributed with more than 38 peer-reviewed publications and 55 abstract presented at international meetings. Currently I am looking for a placement and I confident that with my extensive scientific background, ability to work independently and to interact as a team player in multidisciplinary team. The accompanying resume provides additional information regarding my background, research interest and technical skills.

Visa Status: US - Green Card Holder

Technical Skills

Cell Culture techniques: Primary cell isolation and handling of different cell lines including monolayer and suspension cells, media preparation, subculture propagation, and trouble shooting in cell culture, cell dissociation and cell freezing.

Centrifugation: Isolation of mitochondria, microsomes from different organs. Separation of blood samples from plasma, RBC, PBMC, monocytes and lymphocytes. Electrophoresis and Immunoblotting: Western blot analysis using SDS-PAGE electrophoresis for proteins and using Agarose electrophoresis for DNA and RNA. Molecular Biology Techniques: Macromolecule isolations: Genomic DNA and RNA isolation from various cells, transformation, plasmid isolation and qRT- PCR. Immunology: Separation and generation of dentridic cells (DC) and macrophage (McP) from PBMC. Immune cell functional assays such as ELISpot, proliferation and cytotoxicity ELISA: In vitro cell cytotoxicity assays, hormone assays, cytokine and chemokine Assays. Biomarker Screening using Next-Generation Sequencing: Circulating miRNA from plasma as well as plasma exosome and library preparation for NGS to identify the biomarker signature of brain disorder.

Radioimmuno Assays: Radioisotope binding assays (e.g., 3H and C14). Flow Cytometry: Handling on flow cytometry in various analysis (e.g., DNA content and, mitochondrial transmembrane potential, Annexin V staining and cell viability experiments using Acridine Orange, PI) and ROS estimation (e.g., H2O2, O2 -) etc. Microscopic: Apoptosis - DNA fragmentation analysis using fluorescent probes by microscope. Electroporation Techniques: Transfection of DNA in to Gonadotroph cells. Immunocytochemistry: Cell culture staining and photomicrography and related dark room techniques. Samikkannu Thangavel Ph.D

1245 N Kendall Dr,

APT # F-210

Miami, Florida- 33176

Phone: 305-***-****

E-mail: ******@*****.***

Isolation and Purification of Proteins: Isolation, purification and characterization of protein from natural products in marine organisms by gel filtration, ion exchange and HPLC. Animal Studies: Experience and skills with laboratory rodent care, handle, and drug administration by different routes like PO, IP, IV, IM and tissue sample collection. Biochemical Analysis: In vitro and in vivo quantification of enzymatic and protein analysis. Culture and Propagation of HIV-virus: Hands on experience in HIV virus culture and propagations and working BSL1/2 laboratory setting.

Research Experience

Assistant Professor (Research), Florida International University, Miami, FL 2007-At Present. Major focus in HIV Infection and Illicit Drugs (cocaine, METH, Heroin and Alcohol) induced immune and neuronal toxicity. These research projects are investigating in HIV infection and drugs of abuse impact blood brain barrier (BBB), inflammation, miRNA, cell signaling and metabolic dysfunction lead immune and neuronal toxicity in in vitro, ex vivo as well as patients’ blood samples from HIV positive and drug abuse subjects. (Published 20 manuscripts). Research Associate, Oklahoma University, 2006-2007

Project: Examined the glucose and free fatty acids impact metabolic dysfunction and signaling mechanism of energy sensor AMPK protein in mouse aortic endothelial cells, supported by NIH. This research involved in in vito and animal model studies on FFA impacts energy sensor AMPK mediated metabolic dysfunctions.

Post-Doctoral Research Associate, Texas Tech University, 2003-2006

Worked as NIND project in successfully and independently accomplished two projects: Project: High Glucose induced oxidative stress and renal cell injury, supported by NIH. This research in acute and chronic exposure of high glucose induced oxidative stress impact cell proliferation and apoptosis-signaling mechanism in MAPK and NFκB leads renal toxicity. (Published 2- Manuscripts). Post-Doctoral Fellow, Academia Sinica, Taipei, Taiwan, 2000-2003

Project: Investigated the role of arsenic induced genotoxicity mechanisms in human leukemia cells, supported by NSC. This research project was focused on arsenic (MMA, DMA & As2O3) induced NO2, DNA strand brake and Pyruvate Dehydrogenease (PDH) mediated toxicity. (Published 3- Manuscripts).

Research Associate (ICMR Project), University of Madras, India, 1997-1998

Performed the isolation, purification and biochemical studies with bioactive compound from marine animal Aplysia benedicti (Sea-Hare). This research project was isolated anti-tumor property from marine animal Sea Hare and tested with sarcoma 180 cancer cell line in in vitro model system. Ph.D dissertation Work, University of Madras, India

PhD Thesis: Biochemical Studies on the Toxicity of Methacrylonitrile in rats. In summary, of my work is gavage administration of methacrylonitrile to rats resulted in toxic effects of serum and Lung damages altered redox expression and thiol modification impact lung mitochondrial microsome, RBC and plasma enzymes (LDH, LP, ALP, GSH/GSSG, SOD, CAT, GPx and histological observation of lung tissue. (Published- 4 Manuscripts).

Proven ability to grasp new concepts, methods, and ideas. Demonstrated the ability to excel in both independent and team environments.

Analytical Skills : Flowcytometry, Fluorescent microscopy, UV/Visible and Fluorescence spectrophotometer and plate reader

Software Skills: Microsoft Word, Microsoft Excel, Microsoft Power Point, GraphPad Prizm and Sigma Plot for statistical analysis.

Education:

Ph.D., Biochemical Toxicology; M.Sc., Life Science; B.Sc., Life Science 40-Peer Referred Articles (700+ cited; IF: 110) and 55-Poster Presentations. Selected Peer-Reviewed Publications

1. Thangavel Samikkannu, et al. (2015). HIV Subtypes and Dopaminergic Function: Interactive Role of Methamphetamine. Scientific Reports; 5: 11130. 2. Thangavel Samikkannu, et al. (2015). HIV and Morphine Induced Oxidative Stress: Role in Cell Cycle Regulation. Front Microbial; 614(6):1-8.

3. Thangavel Samikkannu, et al. (2014). HIV-1 Subtypes B and C Tat Differentially Impact Synaptic Plasticity Expression and Implicates HIV-Associated Neurocognitive Disorder. Current HIV Research. 2014; 12(6):397-405.

4. Thangavel Samikkannu, et al. (2014). Immunopathogenesis of HIV Infection in Cocaine Users: Role of Arachidonic Acid. PLOS one 29; 9(8):e106348. 5. Thangavel Samikkannu, et al. (2014). Immuno-Neuropathogenesis of HIV-1 Clade B and C: Role of Redox Expression and Thiol Modification. Free Rad Med Biol 69: 136-144. 6. Thangavel Samikkannu, et al (2013). HIV Infection and Drugs of Abuse: Role of Acute Phase Proteins. Journal of Neuroinflammation. 10: 113.

7. Kurapati V.K. Rao et al (2013). Ashwagandha (Withania somnifera) Reverses β-Amyloid1-42 Induced Toxicity in Human Neuronal Cells: Implications in HIV-associated neurocognitive disorders

(HAND)". PLOS one 2013; 8(10):e77624.

8. Agudelo M, et al (2013). Differential Expression of Cannabinoid Genes in Alcohol Users. Drug and Alcohol Dependence-2013; 133(2):789-93.

9. Samikkannu.T, et al (2006). Acute effect of High glucose on long-term cell growth: A Role for transient glucose increase in Proximal Tubule cell injury. Am J Physiol Renal Physiol. 291(1):F162-75. 10. Samikkannu T, et al (2003) Reactive oxygen species are involved in arsenic trioxide inhibition of pyruvate dehydrogenase activity. Chem Res Toxicol, 16, 409-14. 11. Samikkannu, T. and S. Niranjali Devaraj (1997). Effect of Methacrylonitrile on rat lung antioxidant enzymes, Bull. Environ. Contam. Toxicol, 59, 894-900

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