Ming Chen, Ph.D.

*** ******** ******, *** *******, MA 02740

Email: acym0v@r.postjobfree.com

Telephone: 508-***-**** (c)

Summary:

My background includes five years of experience working as a research fellow/associate research

scientist in both hospital and academic positions. Highlights of my credentials include:

Experience in biomaterial characterization and fabrication; interactions between

biomaterial and cells/tissues/organs;

Experience in using nano particles nano fibers for drug delivery;

Comprehensive knowledge of molecular and cell biology, familiar with state of the art

molecular and cell biology techniques;

A proven track record of peer review publications.

Objective:

A challenge position in the field of pharmaceutical/Biotech where my extensive experience and

background can be utilized.

Immigration Status:

US permanent resident (Authorized to work in the U.S., no visa sponsorship needed.)

Professional Experience:

Research Associate:

Department of Molecular and Cell Biology, University of Connecticut (2012-present)

Developed protocols for nucleus receptor of the juvenile hormone Methyl Farnesoate

Detected and analyzed chemical compounds in environmental samples

Mentored and supervised undergraduate students; Train new lab members. Manage

laboratory instrument, maintenance, ordering, and chemical inventory

Associate Research Scientist:

Department of Pharmaceutical Science, Northeastern University (2011-2012)

Developed SKOV-3 i.p, i.v. model

Developed EGFR-targeted nanoemulsion formulation protocols

Formulation characterization assess size, charge and encapsulation efficiency studies

Research Fellow:

Center for Engineering in Medicine, Harvard Medical School & Massachusetts General Hospital

(2009-2011)

Developed an engineered skin substitute that can deliver multiple factors to improve

wound healing response

Histology analysis

Supervised summer students and technicians

Research Assistant:

Biomedical Engineering Department, University of Massachusetts Dartmouth (2003-2009)

Developed non-woven nano scaffolds using polycaprolactone (PCL) for applications in

dermal filling, grafting, and tissue regeneration

Designed a nano fiber spinning setup.

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Optimized all electrospinning structure parameters for cell growth and differentiation

Laboratory Skills:

Materials Characterization: Fiber diameter and distribution measurement, Mercury

porosimeter, Zeta sizer, UV spectrophotometer, DSC, TGA, TMA, HPLC, GC/MS

Materials fabrication: Electrospinning biomaterials (including PCL, PLLA, PLGA, collagen

etc.), Phase separation, Nano/Microspheres for drug delivery, Microfluidizer

Microscopy: Optical microscope, Fluorescent microscope, Laser scanning confocal

microscope (LSCM), Scanning electron microscope (SEM)

Cell Biology: Primary cultures of 3T3 fibroblasts, human Mesenchymal Stem cells, SH-

SY5Y neuroblastoma cells, HpG2 human liver cancer cells, Keratinocytes, SKOV3,

SKOV3TR, A2780, A2780CP human ovarian cancer cells, J774 macrophage cells, Cell

live/dead assay, Cell cytotoxic assay (MTS), Flow cytometry

Molecular Biology: DNA digestion assay, PCR, Western Blot, Protein binding assays

Biochemistry: Immuno-fluorescent and immuno-histochemical staining of tissue and cells

Animal husbandry: Handle mice and rats for wound healing research, Excision and burn

surgery for mice, Subcutaneous tumor xenograft model, Intraperitoneal tumor model,

Isolating cells for establishing cultures, Harvesting organs, Laboratory animal care and

maintenance

Education:

Ph.D, Biomedical Engineering, University of Massachusetts Dartmouth, N. Dartmouth, MA,

USA (2009)

MS, Physics University of Massachusetts Dartmouth, N. Dartmouth, MA, USA (2003)

M.phil, Physics, University of Hong Kong, Hong Kong, China (2000)

BS, Materials Science, Beijing University of Aeronautics and Astronautics, Beijing, China

(1991)

Selected Publications:

Ming Chen, Melissa Przyborowski, Francois Berthiaume. Stem Cells for Skin Tissue

Engineering and Wound Healing Critical Reviews in Biomedical Engineering, Vol.37(4-5),

2009, p399-421.

Ming Chen, Heather Michaud, Sankha Bhowmick. Controlled Vacuum Seeding as a Means

to Generate Uniform Cellular Distribution in Electrospun Polycaprolactone (PCL) Scaffolds

Journal of Biomechanical Engineering, Vol.131 (7), 2009, p0745211-0745218.

Ming Chen, Michael Lovett, Prabir Patra, David Kaplan, Sankha Bhowmick. Effects of

Electrospun Fiber Diameter and Surface Area on Time-dependent Changes in Cell Numbers

Journal of Tissue Engineering and Regenerative Medicine, Vol.(3), 2009, p269-279.

Ming Chen, Prabir Patra, Steven Warner, Sankha Bhowmick. Role of Fiber Diameter in

Adhesion and Proliferation of NIH 3T3 Fibroblast on Electrospun Polycaprolactone

Scaffolds Tissue Engineering Vol.13(3), 2007, p579-587

Ming Chen, Prabir Patra, Steven Warner, Sankha Bhowmick. Optimization of

Electrospinning Process Parameters for Tissue Engineering Scaffolds Biophysical Reviews

and Letters Vol.1(2), 2006, p153-178

References:

Available upon request.

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