Andrew Huang

** ****** ****, *******, ** *****

516-***-****

Email: acyjhg@r.postjobfree.com

Objective: A clinical laboratory technologist, specialist, supervisor in molecular, chemistry,

hematology, or cytogenetics

Summary

Eight years as a clinical laboratory technologist in molecular diagnostics, chemistry and hematology

Ten years of experience in molecular and cytogenetic cancer research

Demonstrated competence in establishing molecular and cytogenetic assays

Excellent problem solving ability

Skills

DNA, RNA and protein extraction

Polymerase chain reaction (PCR), include real time PCR, conventional PCR and DOP-PCR

Fluorescence in situ hybridization (FISH) and karyotyping

Comparative genomic hybridization (CGH)

Cell culture and bacterial culture

Polyacrylamide and agarose gel electrophoreses and Western blot

Phlebotomy skill in venipuncture and finger stick

Performed blood smears and CBC analysis with differential

Familiar with Cerner Millennium and MEDITECH systems

Proficient in Microsoft Office, especially Excel

Professional Experience

Experience in molecular diagnosis

Optimized DNA extraction protocols, which increased sensitivity up to100 fold

Modified a real time PCR protocol, saving 40% cost of the assay

Extracted DNA from various clinical specimens, including blood, swab, aqueous

and vitreous humor, fresh tissue, and formalin-fixed paraffin-embedded tissue

Established SOPs, three of which were approved by NYSDOH in clinical diagnosis

Real time PCR assays for detecting Herpes viruses including HSV, CMV, VZV,

and EBV; acanthamoeba, and toxoplasma gondii

Experience in chemistry testing

Automated testing: BMP, CMP, lipid panel, thyroid function panel, and PTH assay

Manual testing: urine pregnancy test, urine rapid tox test, sickle cell screen, HIV screen,

and serum acetone test

Point of care testing: BMP by iSTAT and blood glucose level by Precision Xceed Pro

system

Experience in hematology testing

Automated testing: Blood smear staining and CBC analysis

Manual testing: WBC differential, ESR test, fecal occult blood test, plasma replacement

test, and WBC dilution test

Performed phlebotomy and prepared blood smears

Blood bank: received, issued, and canceled blood products

Experience in cytogenetics

FISH

Designed, isolated, and labeled FISH probes

Optimized FISH protocols for gene expression studies and oncology analyses

FISH analyses including probes for the detection of deletion, duplication, amplification, fusion, and break apart

CGH

Labeled CGH probes

Cultured peripheral blood lymphocytes

Harvested lymphocytes from the culture

Prepared metaphase slides

CGH analysis and Karyotyping

Improved CGH in methodology, resulted in the capability of conducting cytogenetic analysis with a few cells

Quality control and quality assurance

Fulfilled CAP and NYSDOH proficiency tests

Performed QC tests based on NYSDOH and CAP guidelines

Ensured accurate and complete documentation for inspection

Research and Teaching Experience

Applied CGH, FISH, and electrophoreses to head and neck cancer research

Conducted gene expression studies by real time PCR and Western blot

Trained new technologists and postdoctoral research fellows in molecular and cytogenetic assays by using PCR, FISH and CGH

Employment

Northwell Health, New Hyde Park, NY

Clinical Laboratory Technologist 2016

New York Eye and Ear Infirmary, New York, NY

Clinical Laboratory Technologist 2008-2016

Research Scientist 1998-2008

Memorial Sloan-Kettering Cancer Center, New York, NY

Research Fellow 1995-1998

Education

New York State Clinical Laboratory Technologist

Molecular Biology Technologist (ASCP)

MD Youjiang Medical University for Nationalities, Baise, China

MS (Biochemistry) Tongji Medical University, Wuhan, China

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