Clinical Laboratory Technologist
Resume:
Andrew Huang
** ****** ****, *******, ** *****
Email: ********@*****.***
Objective: A clinical laboratory technologist, specialist, supervisor in molecular, chemistry,
hematology, or cytogenetics
Summary
Eight years as a clinical laboratory technologist in molecular diagnostics, chemistry and hematology
Ten years of experience in molecular and cytogenetic cancer research
Demonstrated competence in establishing molecular and cytogenetic assays
Excellent problem solving ability
Skills
DNA, RNA and protein extraction
Polymerase chain reaction (PCR), include real time PCR, conventional PCR and DOP-PCR
Fluorescence in situ hybridization (FISH) and karyotyping
Comparative genomic hybridization (CGH)
Cell culture and bacterial culture
Polyacrylamide and agarose gel electrophoreses and Western blot
Phlebotomy skill in venipuncture and finger stick
Performed blood smears and CBC analysis with differential
Familiar with Cerner Millennium and MEDITECH systems
Proficient in Microsoft Office, especially Excel
Professional Experience
Experience in molecular diagnosis
Optimized DNA extraction protocols, which increased sensitivity up to100 fold
Modified a real time PCR protocol, saving 40% cost of the assay
Extracted DNA from various clinical specimens, including blood, swab, aqueous
and vitreous humor, fresh tissue, and formalin-fixed paraffin-embedded tissue
Established SOPs, three of which were approved by NYSDOH in clinical diagnosis
Real time PCR assays for detecting Herpes viruses including HSV, CMV, VZV,
and EBV; acanthamoeba, and toxoplasma gondii
Experience in chemistry testing
Automated testing: BMP, CMP, lipid panel, thyroid function panel, and PTH assay
Manual testing: urine pregnancy test, urine rapid tox test, sickle cell screen, HIV screen,
and serum acetone test
Point of care testing: BMP by iSTAT and blood glucose level by Precision Xceed Pro
system
Experience in hematology testing
Automated testing: Blood smear staining and CBC analysis
Manual testing: WBC differential, ESR test, fecal occult blood test, plasma replacement
test, and WBC dilution test
Performed phlebotomy and prepared blood smears
Blood bank: received, issued, and canceled blood products
Experience in cytogenetics
FISH
Designed, isolated, and labeled FISH probes
Optimized FISH protocols for gene expression studies and oncology analyses
FISH analyses including probes for the detection of deletion, duplication, amplification, fusion, and break apart
CGH
Labeled CGH probes
Cultured peripheral blood lymphocytes
Harvested lymphocytes from the culture
Prepared metaphase slides
CGH analysis and Karyotyping
Improved CGH in methodology, resulted in the capability of conducting cytogenetic analysis with a few cells
Quality control and quality assurance
Fulfilled CAP and NYSDOH proficiency tests
Performed QC tests based on NYSDOH and CAP guidelines
Ensured accurate and complete documentation for inspection
Research and Teaching Experience
Applied CGH, FISH, and electrophoreses to head and neck cancer research
Conducted gene expression studies by real time PCR and Western blot
Trained new technologists and postdoctoral research fellows in molecular and cytogenetic assays by using PCR, FISH and CGH
Employment
Northwell Health, New Hyde Park, NY
Clinical Laboratory Technologist 2016
New York Eye and Ear Infirmary, New York, NY
Clinical Laboratory Technologist 2008-2016
Research Scientist 1998-2008
Memorial Sloan-Kettering Cancer Center, New York, NY
Research Fellow 1995-1998
Education
New York State Clinical Laboratory Technologist
Molecular Biology Technologist (ASCP)
MD Youjiang Medical University for Nationalities, Baise, China
MS (Biochemistry) Tongji Medical University, Wuhan, China
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