Assistant Research

MAURIZIO AFFER

**** ******* ***** ***** **

Atlanta, GA 30318

646-***-**** • ************@*****.***

LinkedIn URL

www.linkedin.com/pub/maurizio-affer/2a/383/638/

SUMMARY

Specific Areas of Functional Expertise

My background is in Molecular Biology and Genetics, and in time it has expanded into transgenic mice, genomics and enzyme/protein studies. Currently, my expertise covers basic

(Flow cytometry, Southern, Northern blots, qPCR assays and cloning), and new molecular techniques (genomic recombineering), protein analysis (Western blots) and purification

(Histidine tagged proteins on Ni+ column) plus animal studies (transgenic mice production, breeding, genotyping and dissection).

Strengths, Experience & Personal Attributes

Thanks to more than 10 years of experience as a molecular biologist, working in different institutions and environments, I am a confident presenter at Journal Clubs, and can efficiently write manuscripts and prepare posters and presentations for meetings. For the past two years I have led the training of summer students on a regular basis and enjoy the challenge and responsibility.

I have the ability to work well with others and work independently with minimal supervision. I’m perseverant, curious, open to new ideas and concepts and ready to learn new skills. Language fluency: Italian (native proficiency), English (full professional proficiency) PROFESSIONAL EXPERIENCE

Morehouse School Of Medicine, Atlanta, Georgia 2016-now

• Establishment of a new laboratory dedicated to the development of preclinical studies (Breast and Head and Neck cancer) using conjugated Gold Nanoparticles Research Assistant

Georgia Tech, Atlanta, Georgia 2014-2016

Research Tech IV

• Creation of recombinant mutant forms of Cathepsins (K, S, L and V) with either the enzyme catalytic or the cannibalistic site blocked. Establishment of stable cell lines (293T) expressing the Wild Type or the previous mentioned mutant cathepsins for protein collection and purification; plus establishing the same cell lines with the Trex vector for protein controlled expression. Development/trouble shooting of protocol for His-tagged protein

(Cathepsin) purification.

Mayo Clinic, Scottsdale, Arizona 2009 – 2014

Research Fellow

• Study of MYC disregulation in Multiple Myeloma: cloning and characterization of regulatory elements affecting MYC expression in Multiple Myeloma cell lines via Luciferase assay (see Leukemia, 2014)

Memorial Sloan Kettering, New York, NY 2006 – 2009 Research Associate

• Gene expression profiling of CML cancer stem cells (see Blood Cancer 2011 and J Oncol. 2011)

Einstein College of Medicine, Bronx, NY

2005– 2006

Research Associate

• Study of role of epigenetic in B cell differentiation and in lymphomas by a newly developed DNA methylation array (HELP assay)

Mayo Clinic, Scottsdale, Arizona

2004 – 2005

Research Associate

• Development of three Conditional Knock Out mice targeting different splice variants of the MMSET gene

Weill Medical College of Cornell University, New York, NY 2000– 2004 Research Associate

• Development and analysis of two transgenic mice lines expressing FGFR3 obtained microinjecting a BAC modified by Homologous Recombination EDUCATION

University of Milan

Laurea in Biological Sciences (specialization in Biomolecular Biology) awarded in Milan, Italy Thesis title:

“Physical map of the cytogenetic band q27 of the human X chromosome: 12Mb of DNA cloned in YACs in a single contig. Approaching the transcription unit map."

(Supervisor Dr. I. Zucchi)

Liceo scientifico Elio Vittorini

B.A. (Math, Science)

Milan, ITALY

Computer skills

Software / Applications:

Flow Jo (Cytometric analysis)

MacVector (DNA sequence analysis)

PowerPoint/Prezi (presentation tools)

Adobe Illustrator/Photoshop (graphic editors)

Xcel

Word/EndNote

ImageStudioLite (western blot analysis)

JMP (statistical analysis)

UCSC genome browser/BLAST (genome and sequences analysis tools) MAURIZIO AFFER Page 3

PUBLICATIONS AND PRESENTATIONS

Journal Articles:

1: Affer M, Chesi M, Chen WD, Keats JJ, Demchenko YN, Tamizhmani K, Garbitt VM, Riggs DL, Brents LA, Roschke AV, Van Wier S, Fonseca R, Bergsagel PL, Kuehl WM. Promiscuous MYC locus rearrangements hijack enhancers but mostly super-enhancers to dysregulate MYC expression in multiple myeloma. Leukemia. 2014 Feb 12. doi: 10.1038/leu.2014.70. [Epub ahead of print] PubMed PMID: 24518206. 2: Wisniewski D, Affer M, Willshire J, Clarkson B. Further phenotypic characterization of the primitive lineage- CD34+CD38-CD90+CD45RA- hematopoietic stem cell/progenitor cell sub-population isolated from cord blood, mobilized peripheral blood and patients with chronic myelogenous leukemia. Blood Cancer J. 2011 Sep;1(9):e36. doi: 10.1038/bcj.2011.35. Epub 2011 Sep 30. PubMed PMID: 22829197; PubMed Central PMCID: PMC3255253.

3: Affer M, Dao S, Liu C, Olshen AB, Mo Q, Viale A, Lambek CL, Marr TG, Clarkson BD. Gene Expression Differences between Enriched Normal and Chronic Myelogenous Leukemia Quiescent Stem/Progenitor Cells and Correlations with Biological Abnormalities. J Oncol. 2011;2011:798592. doi: 10.1155/2011/798592. Epub 2011 Feb 23. PubMed PMID: 21436996; PubMed Central PMCID: PMC3062978. 4: Chesi M, Robbiani DF, Sebag M, Chng WJ, Affer M, Tiedemann R, Valdez R, Palmer SE, Haas SS, Stewart AK, Fonseca R, Kremer R, Cattoretti G, Bergsagel PL. AID-dependent activation of a MYC transgene induces multiple myeloma in a conditional mouse model of post-germinal center malignancies. Cancer Cell. 2008 Feb;13(2):167-80. doi: 10.1016/j.ccr.2008.01.007. PubMed PMID: 18242516; PubMed Central PMCID: PMC2255064.

3: Robbiani DF, Colon K, Affer M, Chesi M, Bergsagel PL. Maintained rules of development in a mouse B-cell tumor. Leukemia. 2005 Jul;19(7):1278-80. PubMed PMID: 15843816.

4: Trudel S, Ely S, Farooqi Y, Affer M, Robbiani DF, Chesi M, Bergsagel PL. Inhibition of fibroblast growth factor receptor 3 induces differentiation and apoptosis in t(4;14) myeloma. Blood. 2004 May 1;103(9):3521-8. Epub 2004 Jan 8. PubMed PMID: 14715624.

7: Hol FA, Schepens MT, van Beersum SE, Redolfi E, Affer M, Vezzoni P, Hamel BC, Karnes PS, Mariman EC, Zucchi I. Identification and characterization of an Xq26-q27 duplication in a family with spina bifida and panhypopituitarism suggests the involvement of two distinct genes. Genomics. 2000 Oct 15;69(2):174-81. PubMed PMID: 11031100.

8: Zucchi I, Montagna C, Susani L, Montesano R, Affer M, Zanotti S, Redolfi E, Vezzoni P, Dulbecco R. Genetic dissection of dome formation in a mammary cell line: identification of two genes with opposing action. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13766-70. PubMed PMID: 10570147; PubMed Central PMCID: PMC24139.

MAURIZIO AFFER Page 4

9: Zucchi I, Jones J, Affer M, Montagna C, Redolfi E, Susani L, Vezzoni P, Parvari R, Schlessinger D, Whyte MP, Mumm S. Transcription map of Xq27: candidates for several X-linked diseases. Genomics. 1999 Apr 15;57(2):209-18. PubMed PMID: 10198160.

10: Redolfi E, Pizzuti A, Di Bacco A, Susani L, Labella T, Affer M, Montagna C, Reinbold R, Mumm S, Vezzoni P, Zucchi I. Mapping of the MYCL2 processed gene to Xq22-23 and identification of an additional L MYC-related sequence in Xq27.2. FEBS Lett. 1999 Mar 12;446(2-3):273-7. PubMed PMID: 10100857. 11: Redolfi E, Montagna C, Mumm S, Affer M, Susani L, Reinbold R, Hol F, Vezzoni P, Cimino M, Zucchi I. Identification of CXorf1, a novel intronless gene in Xq27.3, expressed in human hippocampus. DNA Cell Biol. 1998 Dec; 17(12):1009-16. PubMed PMID: 9881668.

Books, Chapters, Reviews:

"X-linked retinitis pigmentosa: current status" Debra K. Breuer, Maurizio Affer, Sten Andreasson, David G.Birch, Gerald A. Fishman, John R. Heckenlively, Suja Hiriyanna, Dennis R. Hoffman, Samuel G. Jacobson, Alan J. Mears, Maria A. Musarella, Elena Redolfi, Paul A. Sieving, Alan F. Wright, Beverly M.Yashar, Ileana Zucchi, Anand Swaroop. New Insights into Retinal Degenerative Diseases

Edited by Anderson et al., Kluwer Academic/Plenum Publishers, 200 Posters:

“A 3C assay in Multiple Myeloma cell lines reveals a novel interaction between the MYC promoter and a 200Kb telomeric CTCF element on Chr8: a new possible mechanism of MYC disregulation” Maurizio Affer, P. Leif Bergsagel August 2 - 7, 2011 FASEB Science Research Conference Hematologic Malignancies Saxtons River, Vermont (USA) Affer M., Dao S., Liu C., Olsen J. A, Mo Q., Viale A., Marr T.G., Clarkson B. “Gene expression profiles of human hematopoietic quiescent (G0)/CD34+ cells from CML patients and control Bone Marrow”

2007 AACR Annual Meeting. Los Angeles Convention Center California, April 13-18, 2007, Los Angeles, USA.

Maurizio Affer, Davide Robbiani, Marta Chesi, Peter Leif Bergsagel “Ectopic fibroblast growth factor receptor 3 (FGFR3) expression mediated by isotype switch recombination” 10th International Myeloma Workshop Sidney Convention and Exhibition Centre, April 10-14, 2005, Sidney, Australia

Davide F. Robbiani, Kaity Colon, Kruti Naik, Helen Nickerson, Maurizio Affer, Marta Chesi, and Peter Leif

Bergsagel Enforced BCL6 Expression Inhibits B Cell Development in Vivo. Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 1535.

MAURIZIO AFFER Page 5

"Combined strategies for genes identification in Xq27-28." Affer M., Frattini A., Faranda S., Susani L., Ruini L., Montagna C., Redolfi E., Vezzoni P., and Zucchi I. Torino 2-3 Novembre, 1995. Telethon convention.

"Caratterizzazione di una duplicazione in una regione del braccio lungo del cromosoma X in pazienti con difetti nellla chiusura del tubo neurale". Affer M., Redolfi E., Montagna C., Susani L., Hol F., Mariman F., Vezzoni P., and Zucchi I. Napoli 20-22 Novembre, 1996. Telethon convention.

"Identification of six genes in Xq24-q28." Faranda S., Affer M. and Vezzoni P. Capri, 11-14 Ottobre, 1997. 10th II GB Meeting.

"Identification of two novel brain-specific genes from a candidate region for X-linked mental disorders in Xq27.3. " E.Redolfi, S. Mumm, M. Affer, M. Cimmino, L. Susani, C. Montagna, T. La Bella, P. Vezzoni and I. Zucchi.

The American Society of Human Genetics. 48th Annual Meeting. October 27-31, 1998. Denver, Colorado.

"Identification of two novel brain brain-specific genes from Xq27.3 A candidate region for X- linked mental ritardation 6 (XLMR6) syndrome. E Redolfi, M. Affer, M. Cimino, S. Mumm, C. Montagna, L. Susani, R. Reinbold, R:C: Melcangi, L. Martini. P. Vezzoni and I. Zucchi. Society for Neuroscience. Annual Meeting. Nov. 7-12, 1998. Los Angeles, Calif.

"Characterization of an Xq27 duplication involved in neural tube closure defect." Zucchi I, Redolfi E., Affer, M., Susani, L., Montagna C. Roma 15-17 Novembre, 1998. Telethon convention.

"Two novel brain-specific genes in Xq27.3, candidate region for neural disorders." E.Redolfi, S. Mumm, M. Affer, M, T. La Bella, M. Cimmino, L. Susani, C. Montagna, P. Vezzoni and I. Zucchi. Applied Genome Research. November 26-27, 1998. Brussels. Zucchi I., Susani L., Ginestra A., Affer M., Redolfi E., Vezzoni P., Dulbecco R. “Differentiation processes in a mammary carcinoma cell line:identification of gene with opposing action”.Keystone Symposia-Advanced in HumanBreast and Prostate Cancer-Nevada-March 19- 24. 2000.

Zucchi I., Albani D., Affer M., Ginestra A., Susani L., Vezzoni P., Dulbecco R. “Molecular dissection of Dome formation in a mammary carcinoma cell line:identification of genes with opposing action”. Gordon Research Conference-Mammary Gland Biology- Il Ciocco-May 21- 26, 2000.

Zucchi I., Affer M.,Ginestra A.,Albani D., Susani L., Valaperta R., Vezzoni P., Dulbecco R. “A comprehensive gene _expression profile of two rat mammary adeno-carcinoma cell lines with distinct differentiation potential”.SAGE 2000 Conference-Baltimora-September 18-20, 2000. MAURIZIO AFFER Page 6

Zucchi I., Albani D., Susani L., Simionati B., Vergani B., Affer M., Valaperta R., Valle G., Vezzoni P., Pilotti S., Dulbecco R. “Gene _expression profilino of micro-dissected cells from invasive ductal carcino(IDC) and its nodal metastases”. SAGE 2001-San Diego California- September 8-11, 2001.

Presentations:

Ectopic fibroblast growth factor receptor 3 (FGFR3) expression mediated by isotype switch recombination Maurizio Affer, Davide Robbiani, Marta Chesi, Peter Leif Bergsagel 46th ASH Annual Meeting, December 4-7, 2004, S. Diego California.

“A mouse model for Multiple Myeloma” A lecture given at the Grand Canyon University, Phoenix, AZ Guest Speaker invited by Kim V. Curlee, Ph. D. Assistant Professor, Biology

Contact this candidate