Research University

Rony Panarsky, M.Sc.Pharm, Ph.D.

***** ****** ******* *****, ** 20832

734-***-**** ********@*******.***

Biomedical & Pharmacological Researcher

Accomplished researcher brings more than a decade of consistent success with:

Cancer Research

Oncology

Next Generation Sequencing

Site-directed Mutagenesis (CRISPR, TALEN)

In Vivo and In Vitro Models

Generation, Reprogramming and Differentiation of Induced Pluripotent Stem Cells (iPSC) and PBMCs

Biochemistry

Molecular & Cellular Biology

Multiple Immunoassays

Genomics

Multicolor Confocal Microscopy

Cell and Tissue Culturing

Animal models

Multicolor flow cytometry analysis & cell sorting (FACS)

Antibodies and Recombinant Protein Expression & Purification

Scientific Writing

Cellular Imaging & Image Processing

Statistical Data Analysis

Education

Doctor of Philosophy (PhD), Biomedical Sciences, Institute of Drug Research, Faculty of Medicine,

Hebrew University, Jerusalem, Israel, 2012

PhD Thesis: Anti-Inflammatory & Neuroprotective Properties of Ladostigil & Its Primary Metabolites in vitro & in vivo.

Accomplishments: Using primary microglia and macrophages cell line identified the anti-inflammatory mechanism of action of novel compounds in the brain

Master of Science in Pharmacy (MScPharm), Pharmacology, Institute of Drug Research, Faculty of Medicine, Hebrew University

Master of Science (M.Sc), Cell & Developmental Biology, Department of Cell & Animal Biology, Hebrew University

Bachelor of Science in Pharmacy (BScPharm), Institute of Drug Research, Faculty of Medicine, Hebrew University

Bachelor of Science (B.Sc), Life Sciences, Hebrew University

Additional Courses, 2016 – Flow Cytometry; Immunochemistry and Monoclonal Antibody Production, NIH

all 2013: Bioinformatics Analysis of Next Generation Sequencing Data, National Institutes of Health (NIH), Bethesda, MD; Cellular Biology of Addiction, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY Epigenetics, NIH

Academic Awards: Cancer Research Training Award (CRTA), National Institutes of Health, 2015

Intramural Research Training Award (IRTA), National Institutes of Health, 2013

Special Dean Awards for Undergraduate Students, Hebrew University, 1999 – 2001

Fellowship for Participation in Cellular Biology of Addiction Course, Cold Spring Harbor Laboratory, 2013

Travel Fellowships for Graduate Students, Hebrew University & Israeli National Institute for Psychobiology

Technical Skills

Molecular Biology: total RNA ribosomal depleting, microarray/Affimetrix platform, cDNA synthesis, hybridization, detection & analysis, semi-quantitative & quantitative PCR, TaqMan assay, differential expression analysis, molecular cloning, southern & northern blotting, footprinting assay, gel shift assays (EMSA), DNA sequencing (Sanger), multiple transfections & reporter gene assays

Precise Gene Editing: CRISPR, TALEN

Biochemistry: chromatography (HPLC) & electrophoresis, immunoblotting, protein expression, purification & analysis, protein complex pull-downs, protein & peptide purification, fluorescence in situ hybridization (FISH), TBARS assay, BCA & Bradford quantification methods

Cell Viability Assessment: LDH assay, MTT assay, JCI method, Caspase activity, SRB assay

High-Throughout Next Generation Sequencing: total RNA, whole transcriptome and whole genome sequencing of animal tissues & cell cultures (Illumina, SOLID4, & Ion Proton platforms)

Cell Culturing (primary & cell lines): isolation & culturing of primary microglia cells, splenocytes, & primary microphages; culturing & handling cancerous cell lines; culturing tissues; blood cell separation

Drug Discovery: protein-protein interactions, target identification, affinity assays, OCR and ECAR (Seahorse Platform)

Generation, Reprogramming & Differentiation of Induced Pluripotent Stem Cells (iPSCs) and PBMCs

Animal Handling & In Vivo Techniques (rodents): brain surgery, subcutaneous implantations, gavage, multiple microsurgical procedures, generating & handling, animal tumor models

Genetic Analysis, Bioinformatics, & Biostatistics: CLC WorkBench 7, special packages such as GCG & David, SPSS, JMP

Immunochemistry: ELISA, ECL, immunohistochemistry, immunofluorescence, immunoprecipitation, multicolor flow cytometry analysis & cell sorting (FACS)

Life Cell Imaging, Confocal & Light Microscopy, Scientific Writing & Presentations

Research Experience

Postdoctoral Fellow Urologic Oncology Branch, Center of Cancer Research, National Cancer Institute, NIH, Bethesda, MD, November 2015 – Present

Investigation of mechanisms by which various renal cancer cells generate energy and produce biomass required for cell survival and proliferation.

Established various methods for drug screening and cytotoxicity

Characterized metabolic profiles of various cancerous cell lines using Seahorse platform

Developed novel approaches of multi-tier cytotoxic treatment of renal cancer cell lines

Postdoctoral Fellow, Laboratory of Neurogenetics, Section of Molecular Neurobiology, National Institute on Alcohol Abuse & Alcoholism, Bethesda, MD, January 2013 – October 2015

Identified a role of a nonsense point mutation in the HTR2B gene in modulating dopaminergic circuitry in the mouse brain

Developed & applied multiple techniques for precise gene editing (CRISPR, TALEN)

Developed & refined the methodology for differentiating & characterizing human iPSCs into dopaminergic neurons using thick Matrigel platform

Determined the gene expression patterns of various murine brain regions

Research Associate, Faculty of Medicine, School of Medicine, Hebrew University, 2007 - 2011

Developed cell & animal models for metastatic acute myeloid leukemia

Research Associate, Alexander Silberman Institute of Life Sciences, Hebrew University, 2001 – 2002

Established & executed methods for expressing & purifying the viral protein E4orf4 in bacterial systems using numerous molecular cloning techniques

Publications & Presentations

15 publications, abstracts, posters, and manuscripts including:

Influence of a HTR2B Stop Codon on Glucagon Homeostasis and Glucose Excursion in Non-Diabetic Men.

Tikkanen R, Saukkonen T, Fex M, Bennet H, Rautiainen MR, Paunio T, Koskinen M, Panarsky R, Bevilacqua L, Sjöberg RL, Tiihonen J, Virkkunen M.

Exp Clin Endocrinol Diabetes. 2016 Oct;124(9):529-534.

The effects of a HTR2B stop codon and testosterone on energy metabolism and beta cell function among antisocial Finnish males.

Tikkanen R, Saukkonen T, Fex M, Bennet H, Rautiainen MR, Paunio T, Koskinen M, Panarsky R, Bevilacqua L, Sjöberg RL, Tiihonen J, Virkkunen M.

J Psychiatr Res. 2016 Oct;81:79-86.

Impulsive alcohol-related risk-behavior and emotional dysregulation among individuals with a serotonin 2B receptor stop codon.

Tikkanen R, Tiihonen J, Rautiainen MR, Paunio T, Bevilacqua L, Panarsky R, Goldman D, Virkkunen M.

Transl Psychiatry. 2015 Nov 17;5:e681.

Dose-dependent effects of ladostigil on microglial activation and cognition in aged rats.

Weinstock M, Bejar C, Schorer-Apelbaum D, Panarsky R, Luques L, Shoham S.

J Neuroimmune Pharmacol. 2013 Mar;8(1):345-55..

Anti-inflammatory effects of ladostigil and its metabolites in aged rat brain and in microglial cells.

Panarsky R, Luques L, Weinstock M.

J Neuroimmune Pharmacol. 2012 Jun;7(2):488-98.

Effectiveness of 17DMAG, a geldanamycin derivative, in murine acute myeloid leukemia.

Panarsky R, Reichert F, Ben-Ishay Z.

Acta Haematol. 2009;121(1):32-6.

Teaching Experience

Teaching Assistant: courses & workshops about pharmaceutical preparation, developmental biology, & advanced biochemistry, Hebrew University & Jerusalem College of Engineering, 2002 – 2004, 2010 - 2012

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