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HEMANT KUMAR VYAS, Ph.D.

Research Scientist VI

Dept. Of Virology and Immunology

Texas Biomedical Research Institute,

San Antonio, Texas, 78227, USA.

acycub@r.postjobfree.com 857-***-****

RESEARCH INTEREST

Product development of antibody based recombinant molecules for cancer diagnosis and therapy.

Role of neutralizing and enhancing antibodies in the establishment of disease and its progression.

Anti-cancer antibodies and chemo-preventive activity of natural compounds. EDUCATIONAL QUALIFICATION

Post-doctoral fellow at Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA.

Ph.D. (Life sciences) University of Rajasthan, Jaipur, Rajasthan (India).

M.Sc. (Biotechnology) Rajasthan Agricultural University, Bikaner, Rajasthan (India).

B.Sc. (Ag) Hons. Rajasthan Agricultural University, Bikaner, Rajasthan (India). RESEARCH EXPERIENCE

August 2013-till date: Working as “Post-doctoral scientist VI” at Texas Biomedical Research Institute, San Antonio, Texas, USA. Investigating vaccine based humoral correlates of protection in vaccine protected primates. I am conducting projects for designing and engineering epitope based antibodies and assess their effectiveness against SHIV virus with a purpose of vaccine development.

May2010-August 2013: Worked as “Post-doctoral researcher” at Dana-Farber Cancer Institute, and Harvard medical School, Boston, Massachusetts, USA I was involved in developing technologies to isolate protection linked epitopes to isolate single B cells expressing neutralizing antibodies and conduct passive immunization studies in rhesus macaques.

August 2009-April 2010: Investigating clinical samples from advanced stage leukemia and lymphoma patients to observe the presence and role of hCG hormone in disease progression. Performed experiments to develop recombinant bispecific antibodies against advanced stage prostate cancer. I was involved in writing SOPs about recombinant protein purification under GLP conditions.

July 2009-August 2004: Conducted research on immunotherapeutics for advanced stage cancers. Developed immunoconjugates effective against advanced stage leukemias and lymphomas (Patent applied). Generated monoclonal antibodies demonstrating 100% cytotoxicity against advanced stage prostate tumors in-vivo (Patent applied). Achieved anti-cancer plantibody generation. RESEARCH PROJECTS

National Institute of Health (NIH), USA and GATES foundation funded projects:

Role of dimeric secretory IgA isotypes in mucosal surfaces againt viral invasion.

Characterization and phenotypic comparison of memory B cells from vaccinated and non-vaccinated rhesus macaques.

Generation of rhesus macaques antibody genome database and its critical analysis with Human IMGT information system database.

Isolation of mimotope specific memory B cells from vaccine protected animals.

Data analysis of surface markers on human and rhesus macaque B cell repertoire. 2

Purification stratagies to purify HIV-1 gp120ΔV1-V4 expressed using recombinant vaccinia virus technology.

Identification of antibody leader sequence regions from rhesus macaque genome.

Generation of recombinant chimeric antibodies from mimotope specific single B cells isolated from SHIV protected animals.

Bioassay development for functional studies of conformation dependent and low/high affinity antibodies in PBMC and cell line based systems.

Department of Biotechnology (DBT), Govt. Of India, funded projects:

Development of therapeutic antibodies against androgen independent prostate cancer.

Study efficacy and synergistic effect of antibody combination on prostate-tumor xenograft mice.

Generation of immunoconjugates with a high affinity antibody to a non-toxic, anti-cancer natural compounds and testing on acute myeloid leukemia clinical samples for its binding and efficacy studies.

Selection and maintenance of highly productive transgenic lines for expression of chimeric recombinant antibodies.

Expression and purification of recombinant chimeric antibodies from transgenic tobacco plants. TECHNICAL PURVIEW

Management Skills:

Managing multiple interlinked projects at a time and coordinating with various collaborators all over the world.

Supervising graduate students under various projects and teaching skills to summer interns from Austria, Germany and other US universities.

Reporting, data presentation, protocol writing, SOP preparation, study design and data management.

Managing work schedule of lab research associates and technical staff and maintaining collaborative work environment within the lab and in the Department.

Laboratory Skills:

Biochemical techniques: min-midi to giga preps, Western blot, protein extraction from cell lines and tissues, RNA DNA preparation for next generation sequencing (NGS) and analysis, affinity and ion exchange chromatography, SDS PAGE, sub-cell fractionation, tissue homogenization and co-IP related techniques; Molecular biology techniques: Single B cell sorting and monoclonal antibody cloning, recombinant protein expression and purification, restriction cloning and Gibson cloning, RT-PCR and PCR; Microbiological techniques: transfection, common bacterial cultures for expression of proteins, Immunological techniques: FACS, ELISA, immunizations, antibody titration; Virology: In vitro HIV/SHIV virus production, titration, neutralization assays; In vivo: passive immunization study, subcutaneous and orthotropic (prostate) tumor model development, cell culture techniques: transcytosis assays, custom made cell based assays on cell lines and primary cells;

Basic Computational Biology/Bioinformatics Skills:

Genome database profiling, primer & probe designing, sequence analysis from different software packages, NGS data analysis, in-silco vector design, translation of nucleotide sequences, editing and data retrieval from chromatograms. Simulation of Gibson assembly and In-fusion HD cloning. Computing Skills

MS-Office-2010, Genome sequence analysis software, SnapGene 3.1.4, VectorNTI, Serial Cloner, Adobe professional, End Note reference manager, GraphPad Prism. Publications: Research papers, chapters and seminars/talks/posters (Citations: 209) 3

1. 11 peer reviewed articles in high impact journals. Google scholar citations:209. https://scholar.google.com/citations?user=WlS7ahYAAAAJ&hl=en 2. Two book chapters.

Eight Seminar talks / posters presented.

PATENTS

“Compositions, kits and methods for generation of immunoglobulin sequences”. WIPO publication no. WO/2013/148256.

“A recombinant antibody linked to drugs for killing of advanced stage cancer cells”. Indian Patent Application No: IP03950 01352/DEL/200*-**-**-****.

“Anti-advanced state prostate cancer composition”. Indian Patent Provisional application Number:IP10194 02369//DEL/200*-**-**-****.

SCIENTIFIC RESPONSIBILITIES

A. ASSOCIATE EDITOR/MEMBER OF EDITORIAL BOARD

Journal of AIDS & Clinical Research (Editor)

Austin Virology and Retrovirology (Associate Editor)

Frontiers (Molecular Biosciences) (Review Editor) B. PEER REVIEWER FOR SCIENTIFIC JOURNALS

Journal of Clinical and Cellular Immunology; PLoS ONE; Journal of Reproductive Immunology; Frontiers in Genetics; Clinical Microbiology; Journal of AIDS and Clinical Research; Journal of Clinical Toxicology. PROFESSIONAL AFFILIATIONS

Society of Biological Chemists (India)-Associate Member

Indian Immunology Society-Associate Member

Postdoctoral association of San Antonio-Associate Member

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