Quality Control Engineering

Raghuvara Yendluri

*** * ***** ****** *** #**, Ruston LA 71270

562-***-**** *****************@*****.***

PROFESSIONAL SUMMARY

Accomplished, professional researcher with 5 years of research experience in drug delivery and formulation development. Developed sustained release formulations employing various analytical, biomedical and pharmaceutical methods. Independent, proactive and productive researcher with excellent communication skills. Able to network effectively with team members to achieve research objectives. Strong writing and reading skills with publication record in major research journals.

EDUCATION

Louisiana Tech University, Ruston, LA

Ph.D in Engineering (Biomedical engineering), Six Sigma-Green Belt August 2017

Dissertation title: Halloysite clay as a nano tubular template for enzyme immobilization and drug delivery.

Louisiana Tech University, Ruston, LA

Master of Science in Molecular Sciences and Nanotechnology November 2013

Thesis title: Enhancement of half-life and circulation time of gold nano shells using artificial reticuloendothelial system blockade.

Vignan Institute of Pharmaceutical Sciences, Nalgonda, India

Bachelor of Pharmacy May 2011

Senior Project: Formulation and evaluation of gastro retentive drug delivery system of metformin hydrochloride floating matrix tablets.

RESEARCH EXPERIENCE

Institute for Micromanufacturing (IfM), Louisiana Tech University, Ruston, LA

Research Assistant at Nano Assembly Lab December 2013 – Present

Developed a novel oral/solid drug delivery formulation (tablet) using halloysite clay nano tubes as a compression excipient and sustained drug release vehicle. Drug (nifedipine) release for more than 20 hours was achieved, which is higher than commercially available formulations.

Encapsulated anti-cancer and anti-hypertensive drugs within halloysite to achieve controlled drug release for up to 5 hours. Extended drug release for more than 12 hours by coating biocompatible polymers, employing layer by layer (LbL) assembly.

Improved brain cancer cell cytotoxicity using Camptothecin encapsulated halloysite. Improved cell uptake by coating drug encapsulated halloysite with natural polyelectrolytes. MTT assay was performed for cell viability studies.

Developed halloysite clay as an immobilization template for enzymes to improve their stability and shelf-life in extreme pH and temperature environment. Developed dual enzyme – halloysite composites as natural anti-bacterial agents. Anti-bacterial activity evaluated in various bacterial cultures.

Developed micro particles of calcium carbonate incorporated with drug encapsulated halloysite for sustained drug release (for >12 hours) applications.

Documented detailed notes related to results, observations and issues related to drug delivery and formulation development projects in lab notebooks.

Prepared and maintained documents for experimental protocols, SOPs and GLP documents for all the research work performed in the lab.

Validated and maintained a variety of analytical equipment and tools in the lab including HPLC, particle size analyzer, UV- Vis and fluorescence spectroscopy.

Center for Biomedical Engineering and Rehabilitation, Louisiana Tech University, Ruston, LA

Research Assistant at Nano Cancer Therapy and Sensing Group February 2012 - November 2013

Developed λ-carrageenan as a non-toxic reticuloendothelial system suppression agent to enhance the in vivo circulation of nano particles as an alternative to conventional particle modification methods.

Improved blood circulation and in vivo half-life of gold nano particles, used for tumor ablation, by intravenously administering λ-carrageenan.

Enhanced the circulation half-life of nano particles to more than 3 hours by reducing their intravenous blood clearance rate to 40%.

Monitored real time pharmacokinetics: uptake, circulation and distribution of gold nano shells in murine model utilizing a multi wavelength pulse plethysmograph.

INDUSTRIAL EXPERIENCE

Biological E Ltd., Hyderabad, India June 2010 – February 2011

Intern at Quality Assurance department

Analyzed and monitored the quality of manufactured tetanus vaccines by performing industrial standard quality control tests.

Documented SOPs, experimental protocols and results related to quality control of vaccines.

Worked in the manufacturing, packaging and labeling plant for vaccines employing GMP.

TECHNICAL SKILLS

1.Instruments: High Performance Liquid Chromatography RP - HPLC (Agilent 1100), Zeta potential & particle size analyzer- DLS (Brookhaven inst.), Thermo Gravimetric Analysis TGA (TA Inst.), Powder X-Ray Diffraction XRD (Bruker), Surface area and Pore size analyzer (Nova 2200e), Tensile testing machine (ADMET inst.), Contact angle measurement (ODA, FDS Corp.)

2.Spectroscopy: UV-Spectroscopy UV-VIS (Agilent), Fluorescent Spectroscopy (Digilab Hitachi F2500), Fourier Transform IR Spectroscopy FT-IR (Agilent), Raman Spectroscopy.

3.Microscopy: Scanning Electron Microscope SEM (Hitachi), Fluorescent microscope

4.Assays: In vitro enzyme assays, PCR, Fluorescent plate reader

5.Formulation methods: Oral (tablet) drug formulation, nano particle/biomaterial composites, drug encapsulation, LbL thin film polymer deposition, controlled/sustained release agents, work with FDA approved polymers, amphiphiles, lyophilization.

6.Pharmaceutical: Tablet punching machine, Tablet Dissolution and Disintegration Apparatus, Tablet hardness tester, in vivo mouse experiments.

7.Micro/Nanofabrication: LbL self-assembly on nano particles and clay nanotubes, enzyme immobilization on nano clay surface, Preparation of clay nano tube incorporated calcium carbonate micro particles, preparation of in vivo scaffolds for tissue engineering.

8.Writing/ speaking Skills: Research and Review papers, conference papers, posters and presentations

9.Software: Windows (XP, Vista, Seven), Microsoft office, HP ChemStation A.06.03 (for HPLC 1100), Omnic 7.3 (for FT-IR), Image J, Minitab17

CERTIFICATIONS

Six Sigma Certification-Green belt. Expertise in statistical data analysis, SPC, control charts, quality control, FMEA, lean manufacturing, DMAIC, MSA, root cause analysis, process and product development, process capability analysis.

PROFESSIONAL AFFILIATIONS

American Chemical Society 2013 – Present

Member

MANAGEMENT

Vice - President, College of Engineering and Science Graduate Student Council, Louisiana Tech University, Ruston, LA. March 2016 - present

Treasurer, Association of Indian Students (AIS) at Louisiana Tech University, Ruston, LA. February 2012 - January 2013

MENTORSHIP

Led a group of 4 graduate students for the completion of a research project required for a graduate level MSNT 506, Nanofabrication by self-assembly class. Spring 2014 - Present

Teaching assistant for undergraduate students, PHYS 209, 210 classes. Fall 2015 – present

JOURNAL PUBLICATIONS / CONFERENCE PRESENTATIONS

Peer-reviewed publications

Tully J., Yendluri R., Lvov Y., “Halloysite Clay Nanotubes for Enzyme Immobilization,” Biomacromolecules, 2015, 17(2), 615 - 621. [DOI: 10.1021/acs.biomac.5b01542]

Sun J., Yendluri R., Liu K., Guo Y., Yan X., Lvov Y., “Enzyme-immobilized clay nanotube–chitosan membranes with sustainable biocatalytic activities,” Physical Chemistry Chemical Physics, 2017, 19, 562 – 567. [DOI: 10.1039/c6cp07450b]

Jin Y., Yendluri R., Chen B., Wang J., Lvov Y., “Composite Microparticles of Halloysite Clay Nanotubes Bound by Calcium Carbonate,” Journal of Colloid and Interface Science, 2015, 466, 254-260 [DOI: 10.1016/j.jcis.2015.12.031]

Naumenko E., Guryanov I., Yendluri R., Lvov Y., Fakhrullin R., “Clay nanotube-biopolymer composite scaffolds for tissue engineering,” Nanoscale, 2016. [DOI: 10.1039/C6NR00641H]

Magaña I., Yendluri R., Adhikari P., Goodrich G., Schwartz J., Sherer E., O'Neal D P., “Suppression of the reticuloendothelial system using λ-carrageenan to prolong the circulation of gold nanoparticles” Therapeutic Delivery, 2015, 6(7), 777-783. [DOI: 10.4155/tde.15.33]

Book Chapter

Minullina R., Tully J., Yendluri R., Chapter 13, “Halloysite Clay Nanotubes for Long Acting Controlled Release of Drugs and Proteins,” Functional Polymer Composites with Nanoclays, Royal society of chemistry, 2016, 354 – 376.

Conference Proceedings

Magaña I., Yendluri R., Adhikari P., Goodrich G., Schwartz J., Sherer E., O'Neal D P., “The Use of Real-Time Optical Feedback to Improve Outcomes,” SPIE Proceedings, 8995, 2014.

Conference presentations

Yendluri R., Kansakar U., Minullina R., Parekh G., Lvov Y, DeCoster M.A. “Cytotoxicity studies on microglia using halloysite nano tubes coated with block co-polymers of polylysine with polyethylene glycol,” 251st ACS national meeting and exposition, San Diego, CA, March 13-17, 2016.

Yendluri R., Panchal A., Pickett K., De Villiers M., Lvov Y., “Natural clay nanotubes sustained drug formulations,” Industry day, Shreveport, LA, October 1st, 2016

Yendluri R., Tully J., Lvov Y., “Functional clay nanotube enzyme composites,” Louisiana Tech University student research symposium, Ruston, LA, February 2015.

Tully J., Yendluri R., Lvov Y., “Enzyme stabilization by adsorption on and into clay nanotubes”, 251st ACS national meeting and exposition, San Diego, CA, March 13-17, 2016.

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