EDUCATION

Rutgers University, Camden NJ

Master of Science, Biology May 2016

Bachelor of Science, Biology May 2014

AWARDS/FELLOWSHIPS

Teaching Assistantship September 2014

Rutgers University, Camden NJ

Carl Storm Minority Scholarship June 2015

Rutgers University, Camden NJ

Honors College Scholarship September 2009

Rutgers University, Camden NJ

WORK EXPERIENCE

Research and Development Associate May 2016-Present

NMS Labs

Developed an immunoassay for Lyme disease using ELISPOT and live cell culturing

Performed sample preparation for isolation of PBMC from whole blood

Developed and validated cell-based immunoassay for the detection of free and bound neutralizing antibodies against Adalimumab and Infliximab

Validated ELISA based screening of patient serum for trough levels of Adalimumab

Performed literature searches to support method development and assisted with method reporting

Performed routine maintenance and troubleshooting of analytical instruments

Adjunct Instructor December 2016-Present

Rowan University Department of Biology & Department of Chemistry

Teaching Assistant September 2014-May 2016

Rutgers Camden Department of Biology

Developed a physical regulator of uropathogenic E. coli infection through substrate manipulation

Analyzed human and mouse cell lines using fluorescent confocal microscopy

Quantified uropathogenic E. coli infection through use of real time PCR

Constructed polyacrylamide gel substrates on chemically modified glass coverslips

Analyzed genetic sequence data for adhesion assays

Planned and organized curriculum for microbiology and ecology teaching laboratories

Constructed teaching laboratory experiments and laboratory manuals for ecology

Lead weekly meetings for prepared reagents and biological materials for both teaching and research laboratories

Laboratory Assistant August 2012-May 2014

Rutgers Camden Department of Biology

Performed extraction from plant material for fractionation and phytochemical profiling

Intraperitoneal injections on mouse models

Analyzed data from open field exploration, light/dark transition, and elevated plus maze tests

Laboratory Assistant September 2011-July 2012

Rutgers Camden Pinelands Field Station

Prepared and stocked laboratory media

Collected soil samples and analyzed chemical composition

Quantified fungal hyphae using compound light microscope

TEACHING EXPERIENCE

Rowan University Department of Biology

Biology 4: Global Ecology Lecture and Lab (CRN: 21850-01)

Rowan University Department of Chemistry

General Chemistry II Lab (CRN: 21994-02)

Rutgers Camden Department of Biology

General Microbiology Lab Spring 2016 (50:120:331)

Microbiology and its Applications (50:120:212) Spring 2015

General Biology Lab I (50:120:107) Fall 2015

General Ecology Lab I (50:120:353) Fall 2014

Rutgers Camden Learning Center – Certified Peer Tutor

General Biology I and II, Organic Chemistry I and II, General Organic Biochemistry, General Microbiology I, Intermediate Algebra, Elementary Algebra, Fundamental Math Systems I, Spanish I

Rutgers Camden Learning Center – Supplemental Instructor

Held weekly review sessions for General Biology I and II

TECHNICAL PROFICIENCIES

Cell culturing Viral transductions

Human bladder epithelial cell lines Maintaining pathogenic bacterial stock

Human embryonic kidney cell lines ELISA

Mouse embryonic fibroblasts ELISPOT

Gel electrophoresis GloMAX Promega

Confocal microscopy Polyacrylamide gel construction

Spectrophotometry Gibson assembly cloning

Fluorescent plate reader Computer-aided drug discovery

Immunofluorescent monoclonal antibody staining Quantitative PCR

Mass spectrometry Reverse transcription PCR

DNA/RNA extraction Microarrays

PUBLICATIONS

Moorthy, S., Keklak, J., and Klein, E. A. (2016). Perspective: Adhesion Mediated Signal Transduction in Bacterial Pathogens. Pathogens. 5 (2): 23-29.

Keklak, J., et al. Dose-responsive effects of orally administered anxiolytic polar extract of Annona muricata Linn. Plant Physiology & Developmental Botany. Vol 1. May 2014.

PUBLISHED ABSTRACTS

Gordon Research Conference – Microbial Adhesion and Signal Transduction July 2015

Global Health Symposium January 2015/2016

Microbiology Symposium – Rutgers University November 2014/2015

Diversity and Inclusion – Passport to Success Conference April 2016

Center for Computational and Integrative Biology Retreat October 2014/2015

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