Resume

Research Scientist

Location:

Denver, CO

Posted:

March 10, 2017

Contact this candidate

Resume:

Nathan K Kaiser Ph.D.

Objective:

I am currently seeking employment as an Application/Research Scientist as part of a team that works on complex and challenging scientific problems.

I was recently employed as a research scientist at the National High Magnetic Field Laboratory, and was an integral part of the team that designed and constructed the 21 tesla FT-ICR mass spectrometer, which is currently the highest performance analytical mass spectrometer in the world. I designed, tested, modified, and implemented novel hardware (Ultra-high vacuum system, ion source, ion optics, and image current detector), electronics (RF voltage sources, noise reduction, circuit development) and software (ITCL). The instrument provides unparalleled analysis of biological, environmental, and petroleum samples that require ultra-high analytical performance. I maintain, troubleshoot, calibrate, and ensure the sophisticated instruments (FT-ICR mass spectrometers) throughout the laboratory operate at peak performance. I designed experimental setups and instrument configuration for external user projects and perform sample and data analysis.

h-index = 17 (Google Scholar, February 23, 2017)

Average Citation per Publication = 26

Education/Training

Post-Doctoral Fellow 2008 – 2012

Florida State University - National High Magnetic Field Laboratory, Tallahassee, FL

(Advisor Alan G. Marshall)

Doctor of Philosophy: Washington State University, Pullman, WA 2003 - 2007

Analytical Chemistry

Dissertation: Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Instrumentation Design and Development: Reduction of Ion Cloud De-phasing and Time-Of-Flight Discrimination (Advisor – James E. Bruce)

Amgen (Internship): Seattle, WA 2006

NIH Biotechnology Training Fellowship 2005-2006

Bachelor of Science: Aquinas College, Grand Rapids, MI 1998 - 2002

Major: Chemistry

Major: Mathematics

Employment

National High Magnetic Field Laboratory, Tallahassee, FL

Ion Cyclotron Resonance Program 2012 - 2016

Research Scientist

Responsibilities

Technique development and instrument configuration for intact protein analysis

Development of the next generation ion image charge detection system which would increase the speed of acquisition by a factor of 3.

Software development for increased instrument functionality and robustness

Design and testing of customized electrical circuits and compatibility with Ultra-High Vacuum

Manuscript preparation for peer-review

Work with graduate students and outside users on proper operation of the 14.5 T and 21 T Velos-FT-ICR hybrid mass spectrometers.

Lead development of a prototype hybrid mass spectrometer for implementation into a 21 Tesla magnet.

Setup and design experiments for user projects as well as run user samples.

Train graduate students and users on the analytical instrumentation in the lab.

Write standard operating procedures for various aspects of instrument operation and maintenance.

Design, implementation, and testing of novel instrumentation hardware and software for improved FT-ICR mass spectrometer performance.

Maintain, troubleshoot, and calibrate the analytical instrumentation throughout the laboratory.

Top Achievements

Design and installation of the 21 tesla FT-ICR mass spectrometer – the highest performance mass spectrometer in the world.

Obtained unprecedented sequence coverage for intact protein characterization >80% sequence coverage for a 29 kDa protein.

Identification and Characterization of Human Proteoforms by Top-Down LC-21 Tesla FT-ICR Mass Spectrometry

Interfaced commercial analytical instrumentation with custom hardware and software to develop a prototype hybrid mass spectrometer

Development of a novel electrical circuit to improved ion detection characteristics

Analyzer cell design for improved analytical figures of merit (resolving power, mass measurement accuracy, and sensitivity).

Worked with instrument manufactures and academic institutions to implement novel ion fragmentation techniques for proteins and peptides

Time-resolved chemical composition from the Deep-Water Horizon Oil Spill in the Gulf of Mexico

Implementation of a novel ion transfer method from the ion accumulation region to the ICR cell increased the observable mass range.

Operation of ICR cell detection parameters led a 3-5-fold increase in dynamic range for complex samples such as petroleum.

Was the first to obtain baseline resolution of an intact therapeutic antibody (>147 kDa), the largest bio-molecule to be isotopically resolved.

Developed mass calibration techniques to reduce systematic error and improve mass measurement accuracy

Design and implemented a cluster ion source to examine fullerenes.

Professional Memberships and Honors

Member

American Society of Mass Spectrometry 2004-present

Colorado Biological Mass Spectrometry Society 2016-present

Honors

Chemistry Graduate Research Student of the year award, WSU 2007

WSU Chemistry Fellowship Award 2003, 2004

John Pogie Award (top senior chemistry student), Aquinas College 2002

NAIA Academic All-American Cross Country 2000, 2001, 2002

NAIA Academic All-American Track and Field 2000, 2001

Publications (Peer-Reviewed)

https://www.ncbi.nlm.nih.gov/sites/myncbi/nathan.kaiser.1/bibliography/48177691/public/?sort=date&direction=ascending

1)Anderson, L.; DeHart, C.; Kaiser, N.K.; Fellers, R.; Smith, D.F.; Greer, J.; LeDuc, R.; Blakney, G.T.; Thomas, P.; Kelleher, N.; Hendrickson, C.L. Identification and Characterization of Human Proteoforms by Top-Down LC-21 Tesla FT-ICR Mass Spectrometry Journal of Proteome Research 2016 (accepted).

2)Hendrickson, C.L.; Quinn, J.P.; Kaiser, N.K.; Smith, D.F.; Blakney, G.T.; Chen, T.; Marshall, A.G.; Weisbrod, C.R.; Beu, S.C. 21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer: A National Resource for Ultrahigh Resolution Mass Analysis Journal of the American Society for Mass Spectrometry 2015, 26 (9), 1626-1632.

3)Mao, L.; Chen, Y.; Xin, Y.; Chen, Y.; Kaiser, N.K.; Marshall, A.G., Wei, X., Collisional Cross Section Measurements for Biomolecules within a High Resolution Fourier transform Ion Cyclotron Resonance Cell. Analytical Chemistry 2015, 87 (8), 4072-4075.

4)Chen, Y.; Leach, F.E.; Kaiser, N.K.; Dang, X.; Ibrahim, Y.M.; Norheim, R.V.; Anderson, G.A.; Smith, R.D.; Marshall, A.G. Improved ion optics for introduction of ions into a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. Journal of Mass Spectrometry 2015, 50 (1), 280-284.

5)Dunk, P.W.; Mulet-Gas, M.; Nakanishi, Y.; Kaiser, N.K.; Rodriguez-Fortea, A.; Shinohara H.; Poblet, J.M.; Marshall, A.G.; Kroto, H.W. Bottom-up formation of endohedral mono-metallofullerenes is directed by charge transfer. Nature Communications 2014, (5).

6)Chen, T.; Beu S.C.; Kaiser, N.K.; Hendrickson, C.L. Note: Optimization circuit for excitation and detection with one pair of electrodes for improved Fourier transform ion cyclotron resonance mass spectrometry. Review of Scientific Instruments 2014, 85 (6), 066107.

7)Kaiser, N.K.; Savory, J.J., Hendrickson, C.L. Controlled ion ejection from an external trap for extended m/z range in FT-ICR mass spectrometry. Journal of the American Society for Mass Spectrometry 2014, 25 (6), 943-949.

8)McKenna, A. M.; Williams, J.T.; Putman, J.C.; Aeppli, C.; Reddy, C.M.; Valentine, D.L.; Lemkau, K.L.; Kellermann, M.Y.; Savory, J.J; Kaiser, N.K.; Marshall, A.G.; Rodgers, R.P. Unprecedented ultrahigh resolution FT-ICR mass spectrometry and parts-per-billion mass accuracy enable direct characterization of nickel and vanadyl porphyrins in petroleum from natural seeps. Energy & Fuels 2014, 28 (4), 2454-2464.

9)Dunk, P.W.; Adjizain, J.J.; Kaiser, N.K.; Quinn, J.P.; Blakney, G.T.; ewels, C.P.; Marshall, A.G.; Kroto, H.W. Metallofullerene and fullerene formation from condensing carbon gas under conditions of stellar outflow and implications to stardust. Proceedings of the national Academy of Sciences 2013, 110 (45), 180**-*****.

10)McKenna, A. M.; Nelson, R. K.; Reddy, C. M.; Savory, J. J.; Kaiser, N. K.; Fitzsimmons, J. E.; Marshall, A. G.; Rodgers, R. P., Expansion of the Analytical Window for Oil Spill Characterization by Ultrahigh Resolution Mass Spectrometry: Beyond Gas Chromatography. Environmental Science & Technology 2013, 47 (13), 7530-7539.

11)Lobodin, V. V.; Savory, J. J.; Kaiser, N. K.; Dunk, P. W.; Marshall, A. G., Charge Reversal Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Journal of the American Society for Mass Spectrometry 2013, 24 (2), 213-221.

12)Marshall A.G. Blakney, G.T.; Chen, T.; Kaiser, N.K.; McKenna, A.M.; Rodgers, R.P.; Ruddy, B.M.; Xian, F. Mass Resolution and mass accuracy: how much is enough? Mass Spectrometry 2013, Special Issue (2) S0009-S0009.

13)Kaiser, N. K.; McKenna, A. M.; Savory, J. J.; Hendrickson, C. L.; Marshall, A. G., Tailored Ion Radius Distribution for Increased Dynamic Range in FT-ICR Mass Analysis of Complex Mixtures. Analytical Chemistry 2013, 85 (1), 265-272.

14)Dunk, P. W.; Rodriguez-Fortea, A.; Kaiser, N. K.; Shinohara, H.; Poblet, J. M.; Kroto, H. W., Formation of Heterofullerenes by Direct Exposure of C-60 to Boron Vapor. Angewandte Chemie-International Edition 2013, 52 (1), 315-319.

15)Dunk, P. W.; Kaiser, N. K.; Mulet-Gas, M.; Rodriguez-Fortea, A.; Poblet, J. M.; Shinohara, H.; Hendrickson, C. L.; Marshall, A. G.; Kroto, H. W., The Smallest Stable Fullerene, M@C-28 (M = Ti, Zr, U): Stabilization and Growth from Carbon Vapor. Journal of the American Chemical Society 2012, 134 (22), 9380-9389.

16)Dunk, P. W.; Kaiser, N. K.; Hendrickson, C. L.; Quinn, J. P.; Ewels, C. P.; Nakanishi, Y.; Sasaki, Y.; Shinohara, H.; Marshall, A. G.; Kroto, H. W., Closed network growth of fullerenes. Nature Communications 2012, 3.

17)Valeja, S. G.; Kaiser, N. K.; Xian, F.; Hendrickson, C. L.; Rouse, J. C.; Marshall, A. G., Unit Mass Baseline Resolution for an Intact 148 kDa Therapeutic Monoclonal Antibody by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Analytical Chemistry 2011, 83 (22), 8391-8395.

18)Savory, J. J.; Kaiser, N. K.; McKenna, A. M.; Xian, F.; Blakney, G. T.; Rodgers, R. P.; Hendrickson, C. L.; Marshall, A. G., Parts-Per-Billion Fourier Transform Ion Cyclotron Resonance Mass Measurement Accuracy with a "Walking" Calibration Equation. Analytical Chemistry 2011, 83 (5), 1732-1736.

19)Ruddy, B. M.; Beu, S. C.; Kaiser, N. K.; Hendrickson, C. L.; Marshall, A. G., Coulombic shielding during ion cyclotron excitation in FT-ICR mass spectrometry. International Journal of Mass Spectrometry 2011, 301 (1-3), 220-223.

20)Kaiser, N. K.; Savory, J. J.; McKenna, A. M.; Quinn, J. P.; Hendrickson, C. L.; Marshall, A. G., Electrically Compensated Fourier Transform Ion Cyclotron Resonance Cell for Complex Mixture Mass Analysis. Analytical Chemistry 2011, 83 (17), 6907-6910.

21)Kaiser, N. K.; Quinn, J. P.; Blakney, G. T.; Hendrickson, C. L.; Marshall, A. G., A Novel 9.4 Tesla FTICR Mass Spectrometer with Improved Sensitivity, Mass Resolution, and Mass Range. Journal of the American Society for Mass Spectrometry 2011, 22 (8), 1343-1351.

22)Yang, L.; Tang, X. T.; Weisbrod, C. R.; Munske, G. R.; Eng, J. K.; von Haller, P. D.; Kaiser, N. K.; Bruce, J. E., A Photocleavable and Mass Spectrometry Identifiable Cross-Linker for Protein Interaction Studies. Analytical Chemistry 2010, 82 (9), 3556-3566.

23)Weisbrod, C. R.; Kaiser, N. K.; Skulason, G. E.; Bruce, J. E., Excite-Coupled Trapping Ring Electrode Cell (eTREC): Radial Trapping Field Control, Linearized Excitation, And Improved Detection. Analytical Chemistry 2010, 82 (14), 6281-6286.

24)Kaiser, N. K.; Skulason, G. E.; Weisbrod, C. R.; Bruce, J. E., A Novel Fourier Transform Ion Cyclotron Resonance Mass Spectrometer with Improved Ion Trapping and Detection Capabilities. Journal of the American Society for Mass Spectrometry 2009, 20 (5), 755-762.

25)Weisbrod, C. R.; Kaiser, N. K.; Skulason, G. E.; Bruce, J. E., Trapping ring electrode cell: A FTICR mass spectrometer cell for improved signal-to-noise and resolving power. Analytical Chemistry 2008, 80 (17), 6545-6553.

26)Kaiser, N. K.; Weisbrod, C. R.; Webb, B. N.; Bruce, J. E., Reduction of axial kinetic energy induced perturbations on observed cyclotron frequency. Journal of the American Society for Mass Spectrometry 2008, 19 (4), 467-478.

27)Kaiser, N. K.; Skulason, G. E.; Weisbrod, C. R.; Wu, S.; Zhang, K.; Prior, D. C.; Buschbach, M. A.; Anderson, G. A.; Bruce, J. E., Restrained ion population transfer: a novel ion transfer method for mass spectrometry. Rapid Communications in Mass Spectrometry 2008, 22 (12), 1955-1964.

28)Zhang, K.; Wu, S.; Tang, X. T.; Kaiser, N. K.; Bruce, J. E., A bifunctional monolithic column for combined protein preconcentration and digestion for high throughput proteomics research. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 2007, 849 (1-2), 223-230.

29)Kaiser, N. K.; Bruce, J. E., Reduction of ion magnetron motion and space charge using radial electric field modulation. International Journal of Mass Spectrometry 2007, 265 (2-3), 271-280.

30)Wu, S.; Zhang, K.; Kaiser, N. K.; Bruce, J. E., Incorporation of a flared inlet capillary tube on a Fourier transform ion cyclotron resonance mass spectrometer. Journal of the American Society for Mass Spectrometry 2006, 17 (6), 772-779.

31)Wu, S.; Kaiser, N. K.; Meng, D.; Anderson, G. A.; Zhang, K.; Bruce, J. E., Increased protein identification capabilities through novel tandem MS calibration strategies. Journal of Proteome Research 2005, 4 (4), 1434-1441.

32)Kaiser, N. K.; Bruce, J. E., Observation of increased ion cyclotron resonance signal duration through electric field perturbations. Analytical Chemistry 2005, 77 (18), 5973-5981.

33)Kaiser, N. K.; Anderson, G. A.; Bruce, J. E., Improved mass accuracy for tandem mass spectrometry. Journal of the American Society for Mass Spectrometry 2005, 16 (4), 463-470.

Patents

Kaiser, Nathan K.; Bruce, James E. Ion-trapping devices providing shaped radial electric field. U.S. Pat. Appl. Publ. (2009), 28 pp.

References

Alan G. Marshall

Professor Chemistry and Biochemistry, Florida State University

acy73v@r.postjobfree.com

Phone: 850-***-****

Christopher L. Hendrickson

Director of the FT-ICR MS Program, National High Magnetic Field Laboratory

acy73v@r.postjobfree.com

Phone: 850-***-****

James E. Bruce