Robert S Rogowski II

**** ********* *****

Odenton, MD 21113

443-***-**** (Cell)

acy6wf@r.postjobfree.com

SUMMARY:

Received my Bachelor’s Degree in Chemistry from Old Dominion University. Fully qualified scientist in both an educational research setting as well as in a private pharmaceutical lab setting. Extensive experience working independently on projects in private, educational and government settings with numerous years of hands on experience in HPLC, LC/Mass Spectrometry, LC/MS/MS, and GC (also GC/MS) which includes both protein and chemical purification techniques. Years of working in and managing a laboratory which have given me the opportunity to be proficient with a wide range of analytical instrumentation. Isolated single molecular compounds and proteins from bacterial cultures, the human body, bovine, snakes and scorpions, just to name a few of achievements. The projects involved within the science field have resulted in numerous publications in peer reviewed scientific journals. Two first author papers in which I wrote myself and also put together each figure using the data I acquired during my research. Also propagated that data into scientific analysis software in order to create informative figures that gave the reader a thorough understanding of the experimental results. Have a great disposition with my colleagues, graduate students and personnel that I have supervised.

SKILLS:

Managed several labs and had independent projects which limited supervision which required the knowledge to troubleshoot problems and determine the correct paths to take to complete the project using scientific methodologies.

Excellent technical writing skills. Two first author papers and numerous second authors in the field of scientific research which was put together using the data acquired in the role of Research Specialist (See PUBLICATIONS below).

Experience with searching for scientific information on projects using the internet for publicly available resources and for writing a summary of search results and findings.

Experience with literature searches and literature review in the following fields of chemistry, toxicology, biochemistry, immunology and epidemiology.

Extensive experience with making spreadsheets, tables, figures and graphs.

Experience with reference archiving systems for publications.

GMP and GLP compliant which includes keeping a detailed lab notebook.

Record data according to established GMPs and SOPs.

Experience with LCQ-MS/MS and Maldi ToF Mass Spectrometry. Extensive and Experienced Skills in HPLC, Gas Chromatography, GC/MS, Liquid Chromatography, and Protein and Chemical Purification Techniques.

Experience with Agilent, Waters, Hitachi and Beckman HPLC Systems and software.

Elisa and Western Blot Applications.

Extensive Computer Skills: Integration of Computer Systems, System Building, Software Application and System Trouble Shooting.

Proficient in the use of all Microsoft Office programs (Word, Excel, Outlook, PowerPoint), imaging software, scientific (SPSS) and statistical software programs.

Able to Troubleshoot and Repair Multiple Analytical Lab Instrumentation, especially HPLC, LC/MS, GC and GC/Mass Spectrometry Equipment.

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PROFESSIONAL EXPERIENCE:

February 2014- Present

Pharmaceuticals International Inc.

Cockeysville, MD

Senior Chemist

Worked in a quality control lab running several HPLC systems used for determining the presence of drugs during the manufacturing process. Main responsibility was to ensure that no residual drug was present in equipment used in the manufacturing process in order for the equipment to be used for new drug manufacturing processes.

Adhered to strict FDA, GMP, GLP and SOP guidelines.

Kept a detailed notebook in order to adhere to FDA, GMP, GLP and SOP guidelines.

August 2013- January 2014

Actavis Pharmaceuticals (Contract Job with TECH USA)

Millersville, MD

Research and Development Chemist

Performed isolation of unknown compounds in generic drugs prepared by Acatvis Pharmaceuticals in order to determine if the identity of theses unknown compounds before they can be released for human consumption on the open market. Used both preparative HPLC and analytical HPLC to isolate unknown compounds. Adhered to strict FDA, GMP, GLP and SOP guidelines. Used Empower software to run both HPLC systems.

Analyzed data using Empower software and excel spreadsheets. Kept a detailed notebook in order to adhere to FDA, GMP, GLP and SOP guidelines.

July 2012- July 2013

Next Breath, LLC (Contract Job with AEROTEK)

Baltimore, MD

HPLC Analytical Chemist

Performed analysis on samples received from Mylan Pharmacueticals using HPLC and adhering to strict FDA, GMP, GLP and SOP guidelines. Ran three different HPLC systems each day which included Waters HClass HPLC and UPLC systems running Empower software.

Analyzed data using Empower software and excel spreadsheets. Kept a detailed notebook in order to adhere to FDA, GMP, GLP and SOP guidelines.

July 2011- July 2013

Becton Dickinson, Dept. of Bioscience (contract with Mindlance, Inc.)

Hunt Valley, MD

Research Scientist I

Performed analysis on samples received from clients using HPLC/Mass Spectrometry facility. Used MALDI ToF Mass spectrometry, LC/Mass Spectrometry, HPLC, GC and GC/MS to analyze samples provided by clients.

Analyzed data using Empower software and excel spreadsheets. Kept a detailed notebook in order to adhere to FDA, GMP, GLP and SOP guidelines.

March 2010-March 2011

Johns Hopkins University School of Medicine, Dept. of Radiology

Baltimore, MD

Research Specialist

Performed multiple metabolite studies in the Division of Nuclear Medicine using Laurel and Galaxy software in conjunction with Agilent HPLC systems.

Performed HPLC of patient samples injected with radioactive drugs labeled with F-18 and C-11 radioactive tracers to determine metabolic rate of each of the drugs in human and primate subjects using the software and HPLC methods mentioned above.

Performed protein binding assays of radio-labeled drugs administered to patients and Baboons.

Analysis of data showing uptake of radio-isotopic drugs in both patients and Baboons using Microsoft Excel.

Proficient in the handling of all radiation safety and hazardous waste.

2005-2009

University of Maryland School of Medicine, Dept. of Physiology

Baltimore, MD

Senior Research Specialist

Isolation and/or purification of Na-pump inhibitors from both human plasma and Bovine adrenal glands using HPLC, HPLC/MS, radio-immunoassays, and radio-receptor assays.

Characterization of the Na-Pump kinetics and function.

Managed the lab which included training graduate students, post-doctoral fellows and medical students on all equipment and procedures. Also ordered all supplies for the lab, handled all radiation safety responsibilities (wipe test surveys, radioactive waste disposal, and tracking all inventory for all our radioactive isotopes). Also was responsible for administrative duties such as purchasing all supplies for the lab and reconciling all purchases each month in the lab. Provided account spreadsheets with invoices which were required each month.

1998-2005

NASA Langley Research Center Structures and Materials Branch

Hampton, VA

Chemical Engineer

Manager of a HPLC/Mass Spectrometry facility which included analysis of compounds from the Structures and Materials Branch at NASA Langley Research Center using MALDI ToF Mass spectrometry, LC/Mass Spectrometry, LC/MS/MS, and HPLC.

Also included Polymer and Chemical Analysis of compounds using high temperature GPC (Gel permeation chromatography), Gas Chromatography and GC/MS.

1987-1997

University of Maryland School of Medicine, Dept. of Physiology

Baltimore, MD

Research Specialist

Managed the lab which included: Training of students and post-doctoral fellows. Ordering all lab supplies. Maintained lab equipment (which included repair and routine maintenance of equipment). Handling all radiation safety and hazardous training responsibilities as well as having full knowledge of all required procedures for both the handling and use of radioactive and hazardous chemicals. Reconciled administrative charges and updated account spreadsheets with invoices which were required each month.

Characterization, structure and function of several neuronal potassium channels using purified toxins from the venom of both snakes and scorpions using analytical chemistry methods, radioactive receptor assays, HPLC, LC/MS and NMR.

Characterization, structure, function and kinetic properties of the sodium/calcium exchanger in brain synaptosomes.

Isolated several toxins to 99.9% purity from venom of both snakes and scorpions.

Expression, purification, isolation, and characterization of recombinant scorpion toxins for characterization of neuronal potassium channels.

Sold distribution rights of two scorpion toxins: Tityustoxin-K-Alpha and Pandinustoxin-K-Alpha to Research Biochemicals International.

EDUCATION:

Old Dominion University, Norfolk, VA. Bachelors of Science Degree, Chemistry, 1984

PUBLICATIONS:

Levandoski M.M., Caffery P.M., Rogowski R.S., Lin Y., Shi Q., and Hawrot E. Recombinant expression of K-Alpha Bungarotoxin in Pichia pastoris facilitates identification of mutant toxins engineered to recognize neuronal nicotinic acetylcholine receptors. J Neurochem., 74, 1279 - 1289 (2000).

Klenk K.C., Tenenholz T.C., Matteson D.R., Rogowski R.S., Blaustein M.P., and Weber D.J. Structure and functional differences of two toxins from the scorpion Pandinus imperator. Proteins 38, 441 - 449 (2000).

Juhng K.N., Kokate T.G., Yamaguchi S., Kim, B.Y., Rogowski R.S., Blaustein M.P., and Rogawski M.A. Induction of seizures by the potent K-Alpha channel-blocking scorpion venom peptide toxins tityustoxin-K-Alpha and pandinustoxin-K-Alpha. Epilepsy Research 1: 1 - 10 (1999).

Tenenholz, T.C., Rogowski R.S., Collins J.H., Blaustein M.P., and Weber D.J. Solution Structure for Pandinus Toxin K-Alpha (PiTX-K-Alpha), a Selective blocker of A-type Potassium Channels., Biochemistry, 36 10:2763-2771 (1999).

Rogowski, R.S., Collins J.H., O'Neill T.J., Gustafson T.A., Werkman T.R., Rogawski M.A., Tenenholz T.C., Weber D.J., and Blaustein M.P. Three new toxins from the scorpion, Pandinus imperator, selectively block certain voltage-gated K+ channels. Molec. Pharmacol., 50:1167-1177 (1998).

Blaustein, M.P., Fontana G., and Rogowski R.S. The Na+/Ca2+ exchanger in rat brain synaptosomes: kinetics and regulation. Ann. N.Y. Acad. Sci. 779:300-317 (1998).

Eccles, C.U., Rogowski R.S., Gu X., Alger B.E., and Blaustein M.P. Tityustoxin-K-Alpha, from scorpion venom, blocks voltage-gated, non-inactivating potassium current in cultured central neurons. Neuropharmacology 33:1523-1528 (1998).

Fontana, G., Rogowski R.S., and Blaustein M.P. Kinetic properties of the sodium/calcium exchanger in rat brain synaptosomes. J. Physiol. 485:349-364 (1997).

Rogowski, R.S., Krueger B.K., Collins J.H., and Blaustein M.P. Tityustoxin-K-Alpha blocks voltage-gated, non-inactivating K+ channels and unblocks inactivating K+ channels by Alpha-dendrotoxin in synaptosomes. Proc. Natl. Acad. Sci., USA 91:1475-1479 (1997).

Werkman, T.R., Gustfason T.A., Rogowsk R.S., Blaustein M.P., and Rogawski M.A. Tityustoxin-K-Alpha, a structurally novel and highly potent K+ channel peptide toxin, interacts with the Alpha-dendrotoxin binding site on the cloned Kv-1.2 K+ channel. Molec. Pharmacol. 44:430-436 (1997).

Blaustein, M.P., Rogowski R.S., Schneider M.J., and Krueger B.K. Polypeptide toxins from the venoms of old world and new world scorpions preferentially block different potassium channels. Molec. Pharmacol., 40:932-942 (1996).

Sorensen, R.G., M.J. Schneider, R.S. Rogowski and M.P. Blaustein. Snake and scorpion neurotoxins as probes of rat brain synaptosomal channels. Potassium Channels: Basic Function and Therapeutic Aspects. T.J. Colatsky, ed., Wiley-Liss, Inc., NY, Pg. 279-301 (1996).

Schneider, M.J., R.S. Rogowski, Krueger B.K., and Blaustein M.P. Charybdotoxin blocks both Ca-activated K channels and Ca-independent voltage-gated K channels in rat brain synaptosomes. FEBS Lett. 250:433-436 (1995).

ABSTRACTS/POSTER PRESENTATIONS:

1992 ASBMB/Biophysical Society. “Purification and Structural Characterization of a Potent K Channel Blocker from the Scorpion Pandinus Imperator”.

Hypertension meeting (2008). “Isolation of Ouabain (Na-Pump inhibitor) from Bovine adrenals using HPLC and Mass Spectrometry.”

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