Assistant Management

MEGHA SARVOTHAMA

*****, **** **** *** **, Apt *, Seattle, WA 98125

acy3ub@r.postjobfree.com Mob: 206-***-****

SUMMARY

* ***** ** ***** ******** experience- Aseptic techniques, bacterial cloning, Protein expression and purification, chromatography, SDS-PAGE, western blotting and lab management.

Trained in BSL-3 (Mycobacterium tuberculosis) and animal work (mice)

Passionate to learn and to teach. Supervised and mentored interns. Strong record keeping and good communication skills.

Worked individually and as part of team. Strong record keeping skills

EDUCATION

Master of Science in Molecular Biology (2007 - 2009)

Bangalore University, India

Bachelor of Science in Biotechnology, Chemistry and Zoology (2004 - 2007)

Bangalore University, India

WORK EXPERIENCE

Benaroya Research Institute (Jul 2015 - Sep 2015)

Seattle, USA

Volunteer in Dr. Steven. F Ziegler’s Lab

Assisted postdocs by collecting blood, ear punches and organ harvesting. Genotyping and tumor counts.

Institute of Systems Biology (Aug 2014 - Mar 2015)

Seattle, USA

Volunteer in Dr. Nitin Baliga’s Lab

Worked with postdocs on Mycobacterium tuberculosis (Mtb) to reduce the kill time for Mtb after treatment with Bedaquiline by creating transcription factor knockout strains.

Aided a senior scientist with his experiments on Yeast by collecting biomass and extracting PHB, staining and counting cells using light and fluorescent microscopy.

Indian Institute of Science (Aug 2010 - Dec 2013)

Bangalore, India

Project Assistant in Dr. Nagasuma Chandra’s Lab:

Characterization of a protein from Mycobacterium tuberculosis which was computationally proved to be a co-target in the resistance machinery.

Indian Institute of Horticultural Research (Oct 2009 - Jul 2010)

Bangalore, India

Technical Assistant in Dr. Sadashiv’s Lab

DNA fingerprinting in Guava and Papaya using SSR markers.

Transgenic in plants; Functional genomics in Tomato involving genomic DNA extraction from plant leaves, PCR and gel electrophoresis.

RESEARCH EXPERIENCE

Laboratory Management: Inventory, Instruments and data maintenance. Prepared reagents for the experiments.

Training: BSL-3 from Center for Infectious Disease Research (CIDR), Animal handling (mice) from University of Washington (UW), Seattle.

Bacterial Work: Culturing Mtb, Serial dilution, Plating, Colony counting (BSL-3), Cloning of Mycobacterial genes in Escherichia coli, Bacterial cell culture (E. coli and M. smegmatis), Purity check of Mycobacterium cultures (Acid fast stain kit)

Animal work (mice): Cheek bleed, Tail snips, Ear punches, Sacrifices, and Organ harvest

Experimental skills: Bacterial cloning, Extraction of soluble and membrane protein, Protein purification by affinity column chromatography, Protein over-expression, and Dialysis and Enzyme assay (NADPH assay and GTPase assay) and Western blotting.

Bacterial Transformation (chemical and electroporation), Plasmid DNA extraction by alkaline lysis method, Genomic DNA extraction, Agarose gel electrophoresis. RNA extraction by Hot Phenol method and using Qiagen RNA extraction kit, RNA gel electrophoresis, ELISA and Primer designing (GeneTool software).

TEACHING EXPERIENCE

Taught experimental procedures such as media preparation, DNA extraction and purification, Cloning and selection to fresh college lecturers.

Mentoring and supervision of undergraduate students during their internship and helped Ph.D. students with their experiments.

Presented a poster on Principle and Applications of DNA Fingerprinting as part of an annual education program for school and college students in IISc.

PUBLICATIONS

Muthu Arumugam, Deepak Anand, Namperumalsamy Vijayarangan, Chandrasekaran Anbukayalvizhi, Megha Rao, Srinivasan Vijay, Haryadi Rajeswari and Parthasarathi Ajit Kumar. “Nucleoside diphosphate kinase gene is expressed through multiple transcripts in Mycobacterium smegmatis.” The International Journal of Microbiology Research pp.-201-210, 2012.

Deepesh Nagarajan, Geeta Deka and Megha Rao. “Design of symmetric TIM barrel proteins from first principles.” BMC Biochemistry (2015).

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