RHIANNON L KILLIAN also published as Rhiannon Nolan

San Diego, CA 92037 858-***-**** acy0i4@r.postjobfree.com OBJECTIVE

A position which challenges me to produce organized, concise, clear, and effective technical documents EDUCATION

Ph.D. Biomedical Sciences, University of California, San Diego, 2011 B.S. General Science, Anthropology Minor, Pennsylvania State University, 1993 EXPERTISE

Writing & Editing

Reviewing, revising, updating, and proofreading technical documents for format and content to ensure accuracy, completeness, and customer satisfaction

Copy editing simple to complex scientific documents for organization, clarity, consistency, syntax, and style

Composing website content for search engine optimization

Developing protocols and SOPs for instrumentation and laboratory procedures

Preparing progress reports using software, prose, graphs, tables, and images

Utilizing text, graphs, tables, and images to publish scientific findings

Conceptualizing, organizing and composing multi-project research proposals Communication & Analysis

Interacting productively with colleagues face to face, by phone, and by email

Designing, performing, documenting, and troubleshooting biological laboratory assays

Analyzing, interpreting, and communicating experimental data

Presenting scientific information orally and in writing Computer

Writing: Microsoft Office (Word, Excel, PowerPoint & Outlook)

Graphics: SigmaPlot, Adobe Photoshop, Adobe Illustrator, Microsoft PowerPoint, ArcGIS

Programming: Elementary C++, SQL, HTML

Subject Knowledge:

Life Sciences

Biology

Biochemistry

Molecular Biology

Cell Biology

Stem Cell Biology

Neurobiology

Biomedical Science

Laboratory Techniques

Biochemistry & Molecular Biology

Polymerase chain reaction (PCR) & primer

design

Gene cloning

Gel electrophoresis

Western blotting

Protein expression & purification

Immunoassays (e.g. ELISAs)

Chromatography

Spectrophotometry

Cell Biology

Bacterial culture

Viral culture

Mammalian sterile tissue culture, transfection,

transduction & differentiation

Flow cytometry

Fluorescence-activated cell sorting (FACS)

Immunofluorescence

Microscopy

Histochemistry

EMPLOYMENT HISTORY

University of California, San Diego – La Jolla, CA 2004-2011 Biomedical Sciences Graduate Assistant

Reviewed, copy-edited and proofread colleague protocols, manuscripts, proposals, and essays to improve logic, organization, clarity, accuracy, detail, and readability

Maintained accurate and complete daily records in laboratory notebooks

Wrote standard operating procedures describing laboratory techniques and equipment use

Compiled progress reports using software, prose, graphs, tables, and images

Composed abstracts and generated poster presentations for scientific conferences

Created and delivered oral presentations describing technical findings

Conceptualized, organized, and composed basic to multi-project research proposals

Devised, organized, researched, wrote, reviewed, revised, formatted, and published doctoral thesis

Authored text, graphs, figures, and tables for scientific manuscripts Los Alamos National Laboratory – Los Alamos, NM 1995-2004 Research Technician

Wrote protocols detailing laboratory procedures and equipment use

Documented daily research progress in laboratory notebooks

Prepared abstracts and poster presentations for scientific conferences

Generated text and graphs for inclusion in patents

Composed text, graphs, figures, and tables for scientific manuscripts Pennsylvania State University - University Park, PA 1990-1993 Undergraduate Research Assistant

Maintained reference data

Wrote an abbreviated guide to using the reference management software ProCite

Prepared reports describing the results of laboratory experiments using graphs and text HONORS & AWARDS

2008 Alzheimer’s Association Young Scholars Award, San Diego Chapter 2006 Trainee, California Institute for Regenerative Medicine Interdisciplinary Stem Cell Training Program at UCSD, University of California, San Diego

2006 Outstanding Poster Award, International Society for Analytical Cytology XXIII Congress, Québec City, CANADA 1992 Education Abroad Exchange Program, The University of Leeds (UK) 1990 National Science Foundation Undergraduate Research Fellowship, Pennsylvania State University 1989 Early College Enrollment after junior high school year, Pennsylvania State University PUBLICATIONS

Almenar-Queralt, A., Falzone, T. L., Shen, Z., Lillo, C., Killian, R. L., Arreola, A. S., Niederst, E. D., Ng, K. S., Kim, S. N., Briggs, S. P., Williams, D. S., & Goldstein, L. S. (2014). UV irradiation accelerates amyloid precursor protein

(APP) processing and disrupts APP axonal transport. Journal of Neuroscience, 34(9), 3320-39.

Killian, R. L., Flippin, J. D., Herrera C., Almenar-Queralt, A. & Goldstein, L. S. B. (2012). Kinesin light chain 1 suppression impairs human embryonic stem cell neural differentiation and amyloid precursor protein metabolism. PLoS One, 7(1), e29755. Retrieved from http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029755.

Killian, R. L. (2011). KLC1 deficiency alters APP and tau levels and impairs neuronal development (Doctoral dissertation). Retrieved from http://escholarship.org/uc/item/4wk3z2cf#page-4.

Yuan, S. H., Martin, J., Elia, J., Flippin, J., Paramban, R. I., Hefferan, M. P., Vidal, J. G., Mu, Y., Killian, R. L., Israel, M. A., Emre, N., Marsala, S., Marsala, M., Gage, F. H., Goldstein, L. S. B., & Carson, C. T. (2011). Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells. PLoS One, 6(3), 17540.

Shah, S. B., Nolan, R., Davis, E., Stokin, G. B., Niesman, I., Canto, I., Glabe C., & Goldstein, L. S. (2009). Examination of potential mechanisms of amyloid-induced defects in neuronal transport. Neurobiology of Disease, 36(1), 11-25.

Kiss, C., Fisher, H., Pesavento, E., Dai, M., Valero, R., Ovecka, M., Nolan, R., Phipps, L., Velappan, N., Chasteen, L., Martinez, J., Waldo, G. S., Pavlik, P., & Bradbury, A. R. M. (2006). Antibody binding loop insertions as diversity elements. Nucleic Acids Research, 34(19), e132.

Goodwin, P. M., Nolan R. L., & Cai, H. (2004). Single-molecule spectroscopy for nucleic acid analysis: a new approach for disease detection and genomic analysis. Current Pharmaceutical Biotechnology, (5)3, 271-8

Nolan, R. L., Cai, H., Nolan, J. P. & Goodwin, P. M. (2003). A simple quenching method for fluorescence background reduction and its application to the direct, quantitative detection of specific mRNA. Analytical Chemistry, 75(22), 6236-6243.

Lauer, S. L., Nolan, R. L., Goldstein, B., & Nolan, J. P. (2002). Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry, 41, 1742-1751.

Ruscetti, T., Newman, J., Peat, T. S., Francis, J., Nolan, R., Terwilliger, T. C., Peterson, S. R., & Lehnert, B. L.

(1998). A non-denaturing purification scheme for the DNA-binding domain of poly(ADP-ribose) polymerase, a structure specific DNA-binding protein. Protein Expression and Purification, 14, 79-86. PATENTS

Cai, H., Goodwin, P. M., Keller, D. A. & Nolan, R. L. (2007). Quenching methods for background reduction in luminescence based probe-target binding assays. USPTO#7,202,036.

Nolan, J. P., Nolan, R.L., Ruscetti, T., & Lehnert, B. S. (2001). Recombinant fluorescent protein calibration standards. USPTO#6,326,157.

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