RHIANNON L KILLIAN also published as Rhiannon Nolan
San Diego, CA 92037 858-***-**** ***************@*****.*** OBJECTIVE
A position which challenges me to produce organized, concise, clear, and effective technical documents EDUCATION
Ph.D. Biomedical Sciences, University of California, San Diego, 2011 B.S. General Science, Anthropology Minor, Pennsylvania State University, 1993 EXPERTISE
Writing & Editing
Reviewing, revising, updating, and proofreading technical documents for format and content to ensure accuracy, completeness, and customer satisfaction
Copy editing simple to complex scientific documents for organization, clarity, consistency, syntax, and style
Composing website content for search engine optimization
Developing protocols and SOPs for instrumentation and laboratory procedures
Preparing progress reports using software, prose, graphs, tables, and images
Utilizing text, graphs, tables, and images to publish scientific findings
Conceptualizing, organizing and composing multi-project research proposals Communication & Analysis
Interacting productively with colleagues face to face, by phone, and by email
Designing, performing, documenting, and troubleshooting biological laboratory assays
Analyzing, interpreting, and communicating experimental data
Presenting scientific information orally and in writing Computer
Writing: Microsoft Office (Word, Excel, PowerPoint & Outlook)
Graphics: SigmaPlot, Adobe Photoshop, Adobe Illustrator, Microsoft PowerPoint, ArcGIS
Programming: Elementary C++, SQL, HTML
Subject Knowledge:
Life Sciences
Biology
Biochemistry
Molecular Biology
Cell Biology
Stem Cell Biology
Neurobiology
Biomedical Science
Laboratory Techniques
Biochemistry & Molecular Biology
Polymerase chain reaction (PCR) & primer
design
Gene cloning
Gel electrophoresis
Western blotting
Protein expression & purification
Immunoassays (e.g. ELISAs)
Chromatography
Spectrophotometry
Cell Biology
Bacterial culture
Viral culture
Mammalian sterile tissue culture, transfection,
transduction & differentiation
Flow cytometry
Fluorescence-activated cell sorting (FACS)
Immunofluorescence
Microscopy
Histochemistry
EMPLOYMENT HISTORY
University of California, San Diego – La Jolla, CA 2004-2011 Biomedical Sciences Graduate Assistant
Reviewed, copy-edited and proofread colleague protocols, manuscripts, proposals, and essays to improve logic, organization, clarity, accuracy, detail, and readability
Maintained accurate and complete daily records in laboratory notebooks
Wrote standard operating procedures describing laboratory techniques and equipment use
Compiled progress reports using software, prose, graphs, tables, and images
Composed abstracts and generated poster presentations for scientific conferences
Created and delivered oral presentations describing technical findings
Conceptualized, organized, and composed basic to multi-project research proposals
Devised, organized, researched, wrote, reviewed, revised, formatted, and published doctoral thesis
Authored text, graphs, figures, and tables for scientific manuscripts Los Alamos National Laboratory – Los Alamos, NM 1995-2004 Research Technician
Wrote protocols detailing laboratory procedures and equipment use
Documented daily research progress in laboratory notebooks
Prepared abstracts and poster presentations for scientific conferences
Generated text and graphs for inclusion in patents
Composed text, graphs, figures, and tables for scientific manuscripts Pennsylvania State University - University Park, PA 1990-1993 Undergraduate Research Assistant
Maintained reference data
Wrote an abbreviated guide to using the reference management software ProCite
Prepared reports describing the results of laboratory experiments using graphs and text HONORS & AWARDS
2008 Alzheimer’s Association Young Scholars Award, San Diego Chapter 2006 Trainee, California Institute for Regenerative Medicine Interdisciplinary Stem Cell Training Program at UCSD, University of California, San Diego
2006 Outstanding Poster Award, International Society for Analytical Cytology XXIII Congress, Québec City, CANADA 1992 Education Abroad Exchange Program, The University of Leeds (UK) 1990 National Science Foundation Undergraduate Research Fellowship, Pennsylvania State University 1989 Early College Enrollment after junior high school year, Pennsylvania State University PUBLICATIONS
Almenar-Queralt, A., Falzone, T. L., Shen, Z., Lillo, C., Killian, R. L., Arreola, A. S., Niederst, E. D., Ng, K. S., Kim, S. N., Briggs, S. P., Williams, D. S., & Goldstein, L. S. (2014). UV irradiation accelerates amyloid precursor protein
(APP) processing and disrupts APP axonal transport. Journal of Neuroscience, 34(9), 3320-39.
Killian, R. L., Flippin, J. D., Herrera C., Almenar-Queralt, A. & Goldstein, L. S. B. (2012). Kinesin light chain 1 suppression impairs human embryonic stem cell neural differentiation and amyloid precursor protein metabolism. PLoS One, 7(1), e29755. Retrieved from http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029755.
Killian, R. L. (2011). KLC1 deficiency alters APP and tau levels and impairs neuronal development (Doctoral dissertation). Retrieved from http://escholarship.org/uc/item/4wk3z2cf#page-4.
Yuan, S. H., Martin, J., Elia, J., Flippin, J., Paramban, R. I., Hefferan, M. P., Vidal, J. G., Mu, Y., Killian, R. L., Israel, M. A., Emre, N., Marsala, S., Marsala, M., Gage, F. H., Goldstein, L. S. B., & Carson, C. T. (2011). Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells. PLoS One, 6(3), 17540.
Shah, S. B., Nolan, R., Davis, E., Stokin, G. B., Niesman, I., Canto, I., Glabe C., & Goldstein, L. S. (2009). Examination of potential mechanisms of amyloid-induced defects in neuronal transport. Neurobiology of Disease, 36(1), 11-25.
Kiss, C., Fisher, H., Pesavento, E., Dai, M., Valero, R., Ovecka, M., Nolan, R., Phipps, L., Velappan, N., Chasteen, L., Martinez, J., Waldo, G. S., Pavlik, P., & Bradbury, A. R. M. (2006). Antibody binding loop insertions as diversity elements. Nucleic Acids Research, 34(19), e132.
Goodwin, P. M., Nolan R. L., & Cai, H. (2004). Single-molecule spectroscopy for nucleic acid analysis: a new approach for disease detection and genomic analysis. Current Pharmaceutical Biotechnology, (5)3, 271-8
Nolan, R. L., Cai, H., Nolan, J. P. & Goodwin, P. M. (2003). A simple quenching method for fluorescence background reduction and its application to the direct, quantitative detection of specific mRNA. Analytical Chemistry, 75(22), 6236-6243.
Lauer, S. L., Nolan, R. L., Goldstein, B., & Nolan, J. P. (2002). Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry, 41, 1742-1751.
Ruscetti, T., Newman, J., Peat, T. S., Francis, J., Nolan, R., Terwilliger, T. C., Peterson, S. R., & Lehnert, B. L.
(1998). A non-denaturing purification scheme for the DNA-binding domain of poly(ADP-ribose) polymerase, a structure specific DNA-binding protein. Protein Expression and Purification, 14, 79-86. PATENTS
Cai, H., Goodwin, P. M., Keller, D. A. & Nolan, R. L. (2007). Quenching methods for background reduction in luminescence based probe-target binding assays. USPTO#7,202,036.
Nolan, J. P., Nolan, R.L., Ruscetti, T., & Lehnert, B. S. (2001). Recombinant fluorescent protein calibration standards. USPTO#6,326,157.