. Pagadala Vijaya Kumari, Flat no ***, Bhavani Enclave Apartments, Kamalanagar, ECIL, Hyderabad 500062, India.

Contact details: Mob: 091-950-***-****

Email:acxz3t@r.postjobfree.com acxz3t@r.postjobfree.com

Seeking a challenging assignment as

Scientist / Project Manager / Senior Research Associate / Consultant / Expert /PROF/Teaching

target sector: Pharmaceutical / Life Sciences / Biotechnology Academic

A highly qualified Biologist with over 18 years of professional and research experience (3 years in USA and 1 year in Taiwan and presently as a Associate Prof in Biotechnology at ETHIOPIA under UNDP Program) with expertise in molecular biology, plant biotechnology, tissue culture and animal cell culture technology, project management and experience in teaching at Universities in India, Taiwan, USA and Ethiopia.

Educational Qualifications

PhD (Biotechnology) with specialization in plant tissue culture, biotechnology & molecular biology from Osmania University in 1997 (with CSIR, Council of Industrial and Scientific Research Senior Research Fellowship).

M Sc. (Botany) with specialization in cytogenetics from Osmania University in 1990

B Ed. (Biology) with specialization in Biology & English from Osmania University in 1988.

B Sc. (Botany, Zoology & Chemistry) from Osmania University in 1987

Trainings & Workshops Attended

EMBO Practical Course on Plant Reproduction: selected for training in Beijing, China in Aug Sep 2004.(one among 20 selected).

Plant Development Arabidopsis Model System: International Workshop held at National Center for Biological Sciences, Indian Institute of Science, Bangalore, India in Dec 1997.

Cyanobacterial DNA Techniques: a Workshop at the Department of Biotechnology, Center for BGA Biofertilizer, Madurai Kamaraj University, Madurai, India in Mar 1994.

Recombinant DNA Techniques: an Internal Workshop at Madurai Kamaraj University, Madurai, India, sponsored by Scigenics, Madras and Life Technologies Inc., USA in Oct 1992.

Pedegogogical Training held at AMBO University Ethiopia from Nov 12 – 18 2012.

Seminar presentation on one of the publication AMBO University May 9 2013.

TEEAL and AGORA electronic library resources Training on online publications Dec 10 -15 – By ITOCA (South Africa) and Cornell University at AMBO University 2014.

Technical Expertise

Skilled in conducting research and techniques in biotechnology.

Highly analytical with ability to learn and use new skills / techniques quickly.

DNA:

Isolation of genomic DNA from bacterial, yeast, plant and mammalian cells.

Extraction and purification of plasmid DNA.

Retrieval of DNA by cloning, sequencing, southern analysis, PCR – single cell PCR (bacteria & yeast), tissue PCR in plants and other PCR techniques.

DNA synthesis and BrDu labeling in cell cultures.

Transformation:

Bacterial & Yeast: Cacl2 and electroporation, agro bacterial transformation in plant systems, transfection in cell cultures.

RNA:

Isolation from bacteria, yeast, plant and mammalian cells by: northern blotting and dot blot, RT-PCR, gene expression by micro array methods.

Promoter:

Promoter and Lac-Z-Fusions (yeast genes)

Promoter replacement by Tet-O-Promoter systems

Linear integration of genes / promoters / plasmids into yeast genome by homologous recombination.

Complementation Test: on yeast & bacteria.

Protein:

Extraction from bacteria, yeast, plants and mammalian cells by western blotting, immunoprecipitation, ELISA, immunohistochemistry, agglutination test, electrophoresis, immunodiffusion.

Assays:

Using spectrophotometer, fluorimetry, luminometry and isotopic methods, MTT assay, -gal assay in yeast system, GC sterol profile, and citrate snthase enzyme assay.

Cell & Tissue Culture:

Bacterial, yeast, plant and mammalian cell cultures (Human & rat smooth muscle cells, Hela cells, Hep2 cells). Freezing and stocks preparations.

Chlamydial Culture:

Chlamydial culture, titer, infection & harvest.

Microscopy:

Phase contrast microscopy, SEM, immunofluorescence, FACS analysis, confocal microscopy.

Others:

Construction of binary vectors for plant systems.

Screening cDNA library, 5’RACE, density centrifugation & database search.

Scientific Talks : Presented scientific talk shows on biotechnology in YuvaVani (All India Radio) - 13 shows from 1998 to 1999

Computer Skills:

Microsoft Office (Word, Excel, PowerPoint), Graphics and Imaging: Adobe Photoshop, C, C++, Oracle, UNIX Languages, Scanning, Database Searches and Internet Searches.

Strengths

Responsible and efficient in laboratory organization and administration.

Punctual and strive to maintain time schedules.

Excellent interpersonal, communication, presentation and leadership qualities.

Ability to easily mingle with people of diverse cultures as also subordinates, peers and seniors.

Good orator with ability to impress and convince others.

Strong in problem solving and can work well independently as well as in a team.

Professional / Research Experience

Present Position

ASSOCIATE PROF

Computational and Natural sciences

Department of Biotechnology

University of AMBO,

AMBO.

ETHIOPIA TILL DATE Under UNDP – United nations developing ProgramME.

GONDAR UNIVERSITY – Department of BIOTECHNOLOGY 2007 – 2010 (LAST Changed job)

Assitant Prof

Prof GM Reddy Research Foundation R&D, Hyderabad

Scientist

Working on a project entitled ‘Survey, collection and nutritional assessment of lesser known edible plant resources used by major tribes of Andhra Pradesh, India) as a Scientist and Principle Investigator.

Department of Surgery, College of Medicine, University of Tennessee Health Center, USA

Guest Scientist)

Worked on a project entitled ‘Ca/muscular dystrophy and cardiomayapathi in hamsters’ - Analysis of Zn / DMS / Cardiomyopathy on Hamsters

Department of Molecular Sciences, University of Tennessee Health Center, USA

Post Doctoral Fellow

Worked on culture, infection and harvest of Chlamydia Sp.

Department of Physiology & Biophysics, University of Tennessee Health Science Center, USA

Post Doctoral Fellow

Worked on vascular remodeling with cPLA2 (cytosolic phospholipase A2) in human and rat smooth muscle cells. Successfully created a new double mutant phenotype by disrupting the Erg 6 gene for the ncr1 mutant, which is responsible for Niemann Pick disease in humans. In yeast, the function of this gene is not known. However, there are speculations that this gene might be involved in sterol trafficking, as confirmed by GC and GC-Mass Spectrometer. Also developed a double mutant phenotype for the arv1 gene (novel gene), which is a mediator of sterol trafficking with a functional human homolog.

Department of Biology, Indiana University / Purdue University at Indianapolis, Indiana, USA

Post Doctoral Research Associate

Worked on transcriptional regulation of 3 C-4 demethylation genes in Saccharomyces and model studies on ncr1 and arv1 genes in Saccharomyces. Studied the roles of Erg 25, 26 and 27 genes in the removal of two methyl groups at C-4 of the sterol molecule of sterol biosynthetic pathway in saccharomyces cervisiae. The demethylation at the C-14 position appears to be the sole target of azole antifungal activities. The main objective was to analyze the 3 C-4 demethylase genes in Saccharomyces to determine the regulatory mechanisms governing these genes and to assess the possibilities of their suppression which may contribute to their resistance to antifungal agents. Achieved a very good -gal activity for the Erg 26 & 27 promoters fused to Lac Z. Further planned study the interactions between Erg 27, 26 and 25 with Erg 7 gene at the transcriptional level.

Institute of Botany, Academia Sinica, Taiwan

Post Doctoral Research Associate

Worked on sucrose synthase gene isolation and characterization in Arabidopsis thaliana, a plant model system. This enzyme is one of the key enzymes responsible for sucrose and carbohydrate metabolism in higher plants. Only 2 genes had been reported previously. Identified 4 genes, including the 2 reported in Arabidopsis. Successfully cloned full-length cDNAs and confirmed for intron splicing and mutations by sequencing the clones.

Center for Cellular & Molecular Biology, Hyderabad, India.

Post Doctoral Fellow

Worked on isolation, cloning and over expression of citrate synthase gene in Arabiodopsis thaliana. The over expression of enzyme citrate synthase leads to uptake of phosphate ions via roots from soil, the excess enzyme emerging from roots acts as a chelating agent. The candidate gene was successfully isolated, sequenced and cloned into a binary vector for plant transformation. Standardized the enzyme assay for this gene. Screened Arabidopsis plants for female gametophytic mutants using transposon mutagenesized lines of Arabidopsis by gene trap and enhancer trap lines.

PhD Dissertation

(1997)

Title: ‘Induction of haploidy by anther culture and microspore culture in Pigeon pea (Cajanus Cajan (L) Mill Sp.)’ a grain legume of tropics and subtropics.

Research focused on haploidy: anther and microspore (which provide excellent experimental system for genetic transformation, mutant cell selection, haploid breeding, study of embryo development at the cellular, molecular & biochemical levels) cultures were employed for the production of haploids from pollen. Haploid chromosome number was confirmed by various experimental methodologies. Factors for callus induction, somatic embryogenesis and regeneration studies from different explants of 2 cultivars and 2 mutants were standardized.

Teaching Experience

Presently teaching at Ethiopia - AMBO UNIVERSITY – Biochemistry and Biotechnology and Molecular biology for Post Graduate students. TILL DATE

. Thought Biotechnology and Biochemistry at GONDAR UNIVERSITY for three Academic years 2007 -2010.

Molecular biology at the Undergraduate and Graduate levels at the Department of Biology, Indiana University-Purdue University at Indianapolis, IN, during November 2000.

Theoretical and practical plant biotechnology at the Masters Degree level at the Department of Botany, Osmania University, Hyderabad, India - July 1996 to Feb 1997.

Genetics course, both theoretical and laboratory sections at the Undergraduate level at the Department of Botany, Nizam College, Osmania University, Hyderabad, India - During academic year 1994.

Publications

1)Pagadala Vijaya Kumari*, Tsegaye B, Kebede N, Elias T and Gemachu G :Significance of Traditional Medicinal Plants used for Treatment of Rabies at Ambo Town. Medicinal and Aromatic Plants - USA (2015 ) Volume 4 • Issue 4 • 1000207.

2) Pagadala Vijaya Kumari : DIRECT SOMATIC EMBRYOGENESIS FROM MATURE LEAVES OF PIGEON PEA (CAJANUS CAJAN L. MILL SP) . Global J Res. Med. Plants & Indigen. Med. Volume 3, Issue 7 July 2014 286–293.

3) Pagadala Vijaya Kumari1*, Yemsrach Mesfin2. TESTING SALT TOLERANCE TO BOOST ON CHICKPEA (CICER ARIETINUM L. MILL SP) BIOMASS / CULTIVATION. Global J Res. Med. Plants & Indigen. Med. Volume 4, Issue 5 May 2015 79–87

4) Sadiya Kaso, Ato Geda Kebede, Pagadala Vijaya Kumari : STUDY ON TRADITIONAL MEDICINAL PLANTS USED FOR CURING SKIN DISEASES IN AMBO TOWN OF ETHIOPIA . Global J Res. Med. Plants & Indigen. Med. Volume 3, Issue 4 April 2014 134–141.

5) Jemal Ali, Pagadala Vijaya Kumari : GENE SILENCING AND ITS APPLICATIONS IN CROP IMPROVEMENT & FUNCTIONAL GENOMICS Global J Res. Med. Plants & Indigen. Med. Volume 3, Issue 3 March 2014 80–90 . Review Article.

6) Virupapuram Vijaybhaskar; Veeraputhiran Subbiah; Jagreet Kaur; Pagadala Vijaya Kumari; Imaran Siddiqi

Identification of a Root-Specific Glycosyltransferase from Arabidopsis and Characterization of its

Promoter - Journal of Biosciences – 2008.

7) P.Vijaya Kumari, Survey collection and nutritional assessment of lesser known adible plant resources used by major tribes of Andhra Pradesh. DBT’s Annual Report letters 2007.

8) Siddiqi I, G Ganesh, J Kaur, V Subbiah and P Vijaya Kumari: ‘Information and tools from Arabidopsis for biotechnology of crop plants in sorghum tissue culture’ - Transformation and Genetic Engineering, Eds N Seetharama and I D Godwin, Science Publishers Ins., New Hampshire, USA. ISBN -1 - 57808 - 218 – 8, 2004.

9) Vijaya Kumari P, B Kavi Kishore and J K Bhalla: ‘In vitro plant regeneration in pigeon pea [Cajanus Cajan (L.) Mill Sp.] Via organogenesis’ - Plant Cell Biotechnology and Molecular Biology, 2 (1&2): 49-56, 2001.

10) P Vijaya Kumari and S Narasimha Chary: ‘Induction of embryogenesis by anther culture of pigeon pea’ - In vitro Cellular and Developmental Biology, Vol 31A, 63A, 1995 at the Congress on Cell and Tissue Culture held in Denver, Colorado, USA in May 1995..

11) P Vijaya Kumari and S Narasimha Chary: ‘Anther culture studies in pigeon pea’ - In vitro Cellular and Developmental Biology, Vol. 30A, 64, 1994 at the Congress on Cell and Tissue Culture held in Columbia, Missouri in Jun 1994.(Abstract).

12) P Vijaya Kumari and S Narasimha Chary: ‘Induction of haploidy by anther culture and isolated microspore cultures of pigeon pea’ - VII International Congress of Plant Tissue and Cell Culture in Firenze, Italy in Jun 1994. (Abstract).

13) S Narasimha Chary and P Vijaya Kumari: ‘Somatic embryogenesis and plant regeneration from leaf explant of Cajanas Cajan (L) Mill SP’ - VII International Congress of Plant Tissue and Cell Culture in Firenze, Italy in Jun 1994.(Abstract).

14) P Vijaya Kumari and S Narasimha Chary: ‘In Vitro plant regeneration through organogenesis in pigeon pea’ - In vitro Cellular and Animal Developmental Biology, Vol. 29A, 73A, 1993. Research Triangle Park, North Carolina.(Abstract).

15) P Vijay Kumari, S Narasimha Chary and J K Bhalla: ‘Organogenesis and somatic embryogenesis in callus cultures of induced mutants of pigeon pea’ - In vitro Cellular and Animal Developmental Biology, Vol. 29A, 75A, 1993. Research Triangle Park, North Carolina.( Abstract).

16) P Vijaya Kumari and S Narasimha Chary: ‘Embryogenesis from isolated microspores of pigeon pea’ - In vitro Cellular and Animal Developmental Biology. Vol. 29A, 73A, 1993. Research Triangle Park, North Carolina.(Abstract).

GUIDED FOR 18 STUDENTS OF B.Sc AND M.Sc ON VARIOUS PROJECTS.

ACADEMIC ACTIVITIES:

1. Developed Curriculum for B.Sc Biological techniques and B.Sc Biotechnology.

2. Involved in organizing the Seminars and Symposiums.

OTHER ACTIVITIES:

1. One of the best Volunteer to help the ORPHAN CHILDREN of WORLD VISION, MOTHER THERESA CHARITY

2. Involved in BLOOD DONATION camps.

3. Community Service – Teaching Biology for School students from 8 – 12 Grade .

4. Helped the students for developing Communication skills and writing.

Dr. PAGADALA VIJAYA KUMARI.

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