Darbar (HD)

HUSSAIN DARBAR

Monmouth Junction, NJ 08852

Cell: 908-***-****

acxxlj@r.postjobfree.com

EDUCATION

Medical University of Lublin Lublin, Poland

Doctor of Medicine February 2007- July 2012

Rutgers University New Brunswick, NJ

Bachelor of Science, Biochemistry September 2002 – May 2005 EXPERIENCE

Musculoskeletal Transplant Foundation-Fresh Tissue Department Edison, NJ Senior Research Associate April 2013 – Present

Responsible for the aseptic processing of long cortical and cancellous bones into various milled, mixed, and treated products

Process and sterilize osteochondral bone and osteoarticular tissue grafts

Develop in house bio burden (IPBB) samples as tissue pieces prepared and cut prior to disinfection

Responsible for the delipidization of bone and soft tissue, milling of fresh cancellous tissue, and demineralization of milled cortical fibers using Hydrochloric acid and sodium phosphate buffer chemicals

Combine demineralized bone fibers and fresh milled cancellous bones with media enriched in solution to keep stem cells from dying, into final products for cryopreservation

Create antibiotic and surfactant solutions to use in the preparation of grafts

Work with biologically hazardous material in an ISO Class 4 clean room environment

Responsible for all quality checks on processed tissue and paperwork prior to release

Perform all post-process functions including reconciliation, testing, inspecting and labeling

Sample testing, measuring, recording and analysis of results including summary reports

Responsible for lab maintenance and calibration of equipment

Collaborate with members of Process Development, Quality Control, and Quality Assurance to develop therapeutic processes and products.

Coordinate daily laboratory tasks

Contribute input toward the revision of Standard Operating Procedures (SOPs) Johnson & Johnson- Department of Pharmacogenomics (PGx) Raritan, NJ Research Associate July 2006 – February 2007

Performed quality control procedures and Genome Wide Association Study on 3,000 DNA samples.

Executed departmental Standard Operating Procedures, for implementing physical and in silico steps, in a customized Laboratory Information Management System (LIMS), used for anonymization of DNA samples from clinical trials.

Performed picogreen assays to determine the concentrations of DNA samples in ninety-six well format

Analyzed DNA quality of human tissue samples utilizing gel electrophoresis.

Trained in database archiving and lab automation

Managed refrigeration and freezing procedures for DNA samples. Celgene Cellular Therapeutics Summit, NJ

Research Technician March 2006 - June 2006

Purified human placentas into fine, thick collagenous material by aseptic techniques.

Extracted collagen from human placentas.

Thawed, grinded, and rinsed human placental material. Darbar (HD)

Performed concentration and de-fibrillation of collagen solution through a trans-membrane pressure channel and filtration through micron filters.

Execute/implement Clinical Production batch records for the following areas: cell culture, cell harvesting, tissue processing (placenta), cryopreservation.

Follow SOP's associated with processing of Clinical Grade Cellular Therapeutics.

Responsible for entering processing data into Batch Records

Proper handling and storage of products into designated freezer or liquid nitrogen tank

Operation of laboratory processing equipment, including Biosafety Cabinets and Clean Rooms

Maintain a clean and safe work environment in accordance with policies/procedures and OSHA safe practices

SKILLS

Experienced in System Tracking Analysis Anonymization Reporting procedures

Experienced in tissue banking, central sterile supply, and surgical techniques

Knowledge of Current Good Manufacturing Practices (CGMP's), and Quality System Regulations

(QSR's)

Experienced in working in a sterile environment/medical field setting

Ability to perform Anonymization of Clinical and PGx data

Polymerase Chain Reaction (PCR); Bacterial transformation; Gel electrophoresis; Picogreen assays

LIMS; Biomek 2000 liquid handling robot; Gemini XS Spectrofluorometer; Illumina Infinium GWAS analysis

Proficient in Autoclaving; Nano-Drop spectrophotometer instrument; Microsoft Word, Excel, Power Point

Ability to read and interpret documents such as safety procedures/rules and standard operating procedures (SOPs).

Educated in American Medical Association Code of Medical Ethics STRENGTHS

Excellent organizational skills and ability to assist in multiple research projects that comply with FDA standards and guidelines

Great interpersonal and communicational skills, which were applied to teaching and mentoring adults and kids of all ages with Autism

Possess strong problem solving and analytical skills.

Enjoy working in collaborative environments with self-motivational skills and excel in developing strong working relationships with peers

Strong work ethics, a dependable and adaptable team player

Have strong scientific knowledge appropriate for processing clinical grade cellular therapeutics

Ability to consistently perform various manual production duties and techniques on time sensitive material with accurate and precise manipulations.

PUBLICATIONS

Sudden Cardiac Arrest Induced by Hypoglycemia and Hypokalemia in a Chronic Alcoholic Patient. Prystupa A, Kucharska K, Darbar H, Parmar N, Parmar R, Załuska W, Mosiewicz J. Journal of Pre-Clinical and Clinical Research, 2010, Volume 4, No 1, 001-010. (www.jpccr.eu)

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