Research Associate
Resume:
Resume
Naimur Razzaque
**** ********** ***** ****** 240-***-****
Bethesda, MD 20814 acxusu@r.postjobfree.com
EDUCATION
****-**** **** ************* ******* Classes Fresno, CA
California State University
1991-1995 BS Biological Sciences University Park, PA
Penn State University
WORK EXPERIENCE
Nov 2015- Aug 2016 Foundation for Advanced Education in the Sciences in Bethesda, MD-
Job duties: Prepared Hela Cell lines along with IMR’s and L929’s to grow
for the purposes of making kits to send out to educational instititutes.
This includes doing karyotype analysis along with fixation of cells on glass
plates to use for staining. Also involved in making various types of media
for maintenance of the cell lines.
Feb-April 2015 Blood Processing Specialist at Medimmune in Gaithersburg, MD-
Job duties: Processing 600 ml or more of human blood from donors at blood
bank to obtain specific target cells like T and B cells for use by scientists for
their research. The cells were further stained and analyzed by flow
cytometry for quality control measures.
May-Nov 2012 Research position at the FDA in Rockville MD-
Job duties: Development and characterization of novel vaccines against
the most virulent types of Malaria in sub-Saharan Africa. Assisted in
DNA cloning, restriction digestion, plasmid preps, agarose gel
electrophoresis, and western blotting.
Project focused on development of vaccine models using resistance plasmids developed through molecular biology techniques to be used in malaria prevalent areas of the world
April 2008- April 2011 ORISE Fellow at Food and Drug Administration-
Job duties: Isolation and characterization of monkey PBMCs through
gradient centrifugation, antibody labeling, magnetic columns,and
flow cytometric studies to determine a target population of cells called
invariant Natural Killer T cells and T regulatory cells involved in the
pathogenesis of Hepatitis A virus infection in rhesus macaques and
humans.
Work involved development of detection assays using various markers to label the surface of invariant Natural Killer T Cells with multicolor immunostaining. The Peripheral Blood Mononuclear cells were isolated using a gradient centrifugation prior to labeling.
The stained cells were read using a BD canto machine.
A secondary project involved isolation and characterization of T
Regulatory cells using magnetic bead columns which bind to
specific receptors on the cell surface and then further analyzing the
cell prep using the BD Canto and Flo Jo Software to determine
the target cells of interest.
January 1999-December 1999 –Researcher at the Food and Drug Administration-
Testing the potency and efficacy of pertussis vaccines using mouse models
Gained hands on experience with Enzyme Linked Immunosorbent Assays(ELISA’s)
Project involved injecting mice with inoculates of pertussis and also a conjugated form of the vaccine and then doing tail bleeds at various time points to see the levels of antibody titer
These bleeds were then further processed using ELISA and colorimetric assays to gauge the level of antibodies produced and determine vaccine efficacy
Lab Skills: Familiar with cell culture maintenance including prior work with neuroblastoma cells, natural killer T cells, and pertussis vaccines. Also prior experience with flow cytometry using BD Canto and Flo Jo software for 3 years, ELISAs, agarose gel electrophoresis, and cell cloning.
References:
Jitesh Vitael, Supervising Science Officer(supervisor)
Foundation for Advanced Education in the Sciences
10 Center Drive, Room 1N241-MSC1115, Bethesda, MD 20892
acxusu@r.postjobfree.com
Gerardo Kaplan, Principal Investigator (former supervisor)
Food and Drug Administration, DETTD
10903 New Hampshire Avenue, Silver Spring MD 20993
acxusu@r.postjobfree.com
Sanjay Kumar, Principal Investigator (former supervisor)
Food and Drug Administration, CBER
10903 New Hampshire Avenue, Silver Spring MD 20993
acxusu@r.postjobfree.com
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