Beverly Lynn Wolgast

SUMMARY

Ph.D. in Synthetic Organic Chemistry plus experience which includes Medicinal and Process chemistry in Pharmaceutical products; Polymer Chemistry and Molecular Biology in DNA release applications; Polymer Chemistry with Stent Applications; Synthesis of phosphothio-oligonucleotides using automation; SAR hit-to-lead; developing a novel synthesis of a 3-benzazapine, Lorcaserin, a novel synthesis of an anti-hypertensive, Enalapril; and Harvard Post-doctoral work in synthesis of alkylmercaptans using photochemistry.

PROFESSIONAL QUALIFICATIONS

DIOMICS CORPORATION, San Diego, California 2014-present

CHIEF OPERATING OFFICER (Polymer Chemistry and Molecular Biology)

Responsible for all operations including laboratory supervision, scale up, manufacturing, hiring and firing personnel, purchasing, accounting, interaction with investors, Diomics’ board, SOP's, and equipment maintenance. Upper management interaction and reporting responsibilities.

Utilized qPCR for assay determination.

Discovered Method to make Hydrophilic Thin Films which enable fingerprint identification and DNA release sufficient for full CODIS alleles.

Determined Method for manufacturing of swabs which pick up and release DNA at high assay.

BW Consulting, San Diego, California 2013-2014

Principal Consultant (Medicinal and Process Organic Chemistry)

Range of services from route scouting for API's, SAR and scaffolds, chemical outsourcing, batch production writing, SOP's, negotiation and obtaining contracts, manufacturing procurement.

PACIFIC WORLD DISCOVERY, San Diego, California 2012-2013

Director of Chemistry and Operations (Medicinal and Process Organic Chemistry)

Responsible for supervision of all personnel, reporting to business owner, member of upper management, report writing, interaction with customers, presentations, compiling reports for customers, business development.

REVA MEDICAL, INC, San Diego, California 2010-2012

Lead Organic Chemist (Process Organic Chemistry, Polymer Chemistry)

Responsible for complete laboratory operations; set-up a new polymer chemistry syntheses program for stent design and hip, knee, spinal and other orthopedic usages.

Compounds include propanediol initiators, lactides, caprolactone, Tin (II) initiators, macromers, Bis(trichloromethyl) carbonate, tyrosine, and des-amino tyrosine (DAT).

ISIS PHARMACEUTICALS, Carlsbad, California 2009-2010

Sr. Process Chemist (Process Organic Chemistry, Chemical Development, RNA si synthesis)

Responsible for improving and validating scale up and improvements to phosphothio oligonucleotide syntheses including:

AKTA 100 Plus Synthesizers and Purifiers programmed for syntheses with Automated Synthesis; Automated Purification.

Responsible for impurity analysis and alternative solvents; Synthesized complements; utilized Unicorn software programmed for synthesis and purification; utilized LCMS techniques and ion extraction for purity/impurity analysis;

Participated in conference calls with collaborators and CRO’s for monomers and final products.

CONSULTANT (CONTRACT CHEMISTRY) 2005-2008

(Research Fellow in Medicinal Chemistry; Scale-Up Chemistry)

GENOMICS INSTITUTE OF THE NOVARTIS RESEARCH FOUNDATION (GNF)

San Diego, California

VERTEX PHARMACEUTICALS, San Diego, California

TAKEDA SAN DIEGO, San Diego, California

Synthesized new molecules with templates of SAR around scaffolds.

Scaled Up advanced chemical intermediates and APIs.

Utilized equipment including Prep HPLC based on MW and or prep TLC for final compound.

Used equipment including ISCO columns, SFC, microwave, LC/MS, and NMR.

Included Chemical synthesis of steps by Buchwald, Palladium, Heck, and Mitsunobu.

Co-authored Two Patents

ARENA PHARMACEUTICALS, San Diego, California 2002-2005

Research Scientist (Process Chemistry, Route-Scouting, Chemical Development)

Researched and developed routes to substituted 3-benzazepines, aromatic ureas, ethers, and halogens, and amino heterocyclic compounds with anti-obesity, anti-diabetes end-points.

Provided chemical intermediates to Medicinal Chemistry (for SAR purposes).

Conducted chemistry using GLP and cGMP techniques; Developed analytical methods.

Carried out Kilo laboratory work; Wrote Technical Reports.

Authored two patents and one paper; Supervised one direct report; Supported CRO operations.

FMC CORPORATION, Princeton, New Jersey 1995-1999

Research Scientist (Process Chemistry, Route-Scouting, Chemical Development)

Researched and developed routes to new herbicides and insecticides (route-scouting, costing, comparison and evaluation of routes, kilo lab work). Authored Patents. Innovation Award.

Lead chemist for sample preparation and pilot plant support of F3686 and F6028. Three Reports.

Performed trouble-shooting and start-up for launch of new herbicide (Sulfentrazone) manufactured in Baltimore, MD (crystallization, recycle, filtrate-recovery).

Supported Plant operations and tollers (CROs) for existing products. Six Sigma Applications.

CELGENE CORPORATION, Warren, New Jersey 1992-1994

Research Scientist (Process Chemistry, Route-Scouting, Chemical Development)

ALKERMES CORPORATION, Cambridge, Massachusetts 1989-1991

Research Scientist (Bioconjugate Chemistry, Enzymatic Digest, amino acid Sequencing, Radiolabeling)

Small Business Innovative Research (SBIR) Grant Funded $49K, First Author, 1991

DURACELL RESEARCH CORPORATION, Needham, Massachusetts 1986-1987

Research Scientist (Polymer Chemistry, Electrochemistry, Lithium Battery Chemistry, Cut-off Switch)

EDUCATION

Post-Doctoral Fellow, Harvard University, Cambridge, Massachusetts 1987-1989

Monolayers on gold; Thin Films; Sputtering; Photochemistry; Synthesis of Alkylmercaptans

Ph.D. Organic Chemistry, Brandeis University, Waltham, Massachusetts 1986

Intramolecular Alkenyl-allene Diels Alder Reactions; [2+2] Cycloadditon Reactions

B.S. Chemistry, Rensselaer Polytechnic Institute, Troy, New York 1981

Minors in Molecular Biology and Business

PATENTS

1.Beverly Wolgast, John Steel, Thomas Kindt, Ryan Lamer, Films for Biologic Analyte Collection and Analysis and Methods of Production and Use Thereof

US Application no. 14/603,755 and PCT/US15/12627 Filed 1/23/2015 (Claiming priority to prov filed 8/15/2014). Issued 01/28/2016.

2.Thomas Kindt, John Steel, Beverly Wolgast, Ryan Lamer, Devices and Kits for Collection, Storage and Analysis of Samples and Methods of Production Thereof

US Application no. 14/700,539 and PCT /US15/28458 Filed 30 April 2015 (Claiming priority to prov filing 5/1/2014). Issued 11/05/2016.

3.Beverly Wolgast, Isaiah Cedillo, Dennis Gorman, Andrew McPherson, “Alternative Solvents for Oligonucleotide Syntheses”, Provisional App. U.S. Patent and Trademark Office October, 2009.

4.Hidehisa Iwata, Yasuhisa Kohara, Sheldon X Cao, Prasuna Guntupalli, Stephen L Gwaltney, David J Hosfield, Yan Liu, Jeffrey A Stafford, Beverly Wolgast: Imidazopyridine compounds. Takeda San Diego, Feb, 28 2012: US 8124617.

5.Zacharia Cheruvallath, Jun Feng, Prasuna Guntupalli, Stephen L Gwaltney, Stephen W Kaldor, Joanne Miura, Mark Sabat, Mingnam Tang, Beverly Wolgast: Glucokinase activators. Takeda San Diego, Oct, 11 2011: US 8034822.

6.Zacharia Cheruvallath, Jun Feng, Prasuna Guntupalli, Stephen L. Gwaltney, Stephen W. Kaldor, Joanne Miura, Mark Sabat, Mingnam Tang, and Beverly Wolgast, “Glucokinase Activators” Takeda San Diego, Sep, 13 2007: US 200********.

7.Tawfik Gharbaoui, John R Fritch, Ashwin M Krishnan, Beverly Wolgast Throop, Naomi S Kato: Processes for preparing aromatic ethers. Arena Pharamaceuticals Oct, 12 2010: US 7812159

8.Gharbaoui Tawfik, Fritch John R, Krishnan Ashwin M, Throop Beverly Wolgast, Kato Naomi S: Processes for preparing 4-(phenoxy-5-methyl-pyrimidin-4-yloxy)piperidine-1-carboxylic acid derivatives and related compounds. Arena Pharm Oct, 3 2007: EP1838697

9.Tawfik Gharbaoui, John R. Fritch, Ashwin Krishnan, Beverly Wolgast Throop, and Naomi Kato, “Synthesis of aromatic ethers”, Arena Pharmaceuticals, US Patent Application Issued July 13, 2006 #200********.

10.Beverly Wolgast, Charles Gilson III, Shelley Aytes, Scott Estrada, Dipanjan Sengupta, Brian Smith, Max Rey and Ulrich Weigl; “Synthesis of 3-benzazapines”, Arena Pharmaceuticals, Filed October 21, 2003. Issued. US Serial No. 11/327,923.

11.Gerald A. Crispino, Beverly Wolgast Burbaum, Mark Diener “Process Improvements Utilizing the Mannich Reaction en route to Herbicide F3686 formation”. Docket # 60123, filed 12/17/97.

12.Beverly Wolgast Burbaum, Chunshi Li, and George W. Matcham "Process for the Stereoselective Preparation of L-Alanyl-L-Proline". Issued June 7, 1994. U.S. Patent No. 5,319,098.

13.Beverly Wolgast, Lyuba Kabalnova, Wade de Polo, Elizabeth Nesseler, “Process for the Preparation and Degradation of Radiopaque, Bioresorbable Polymers for Stent Scaffolds”. In Process.

PUBLICATIONS

1. Discovery of APD356: A potent and selective 5-HT2C receptor agonist for the treatment of obesity. Smith, Brian; Smith, Jeffrey; Chen, Rita; Covel, Jon; Dosa, Peter; Estrada, Scott; Gilson, Charles: Park, Douglas; Prieto, Emily; Schultz, Jeffrey; Tsai, James; Webb, Rob; Aytes, Shelley; Burbaum, Beverly Wolgast; Fritch, John; Sengupta, Dipanjan; Henshilwood, James; Reyes Saldana, Hazel; Thomsen, William; Whelan, Kevin; Manzaghi, Frederique; Bjenning, Christina; Martin, Michael; Chapman, Dennis; Maffuid, Paul; Beeley, Nigel; Chalmers, Derek; Bhan, Dominic. R&D, Arena Pharmaceuticals Inc., San Diego, CA, USA. Abstracts of Papers, 227th ACS National Meeting, Anaheim, CA, United States, March 28-April 1, 2004. MEDI-271. Publisher: American Chemical Society, Washington, D. C. CODEN: 69FGKM Conference Meeting Abstracts written in English. AN2004:226629. CAPLUS

2. Type I Intramolecular Cycloadditions of Vinylketenes. Lee, S.Y.; Kulkarni, Y.S.; Burbaum, Beverly Wolgast; Johnston, M.I.; Snider, B.B. J. Org. Chem. 1988, 53, 1848-1855.

3. Type II Intramolecular [2+2] Cycloaddition Reactions of Alkenes with Vinylketenes Prepared by the Regiospecific Deprotonation of beta, beta-Disubstituted alpha, beta -Unsaturated Acid Chlorides. Snider, B.B.; Ron, E., Burbaum, Beverly Wolgast J. Org. Chem. 1987, 52, 5413-5419.

4. Lewis Acid Induced Asymmetric Prins Reactions of Chiral Acetals with Alkenes. Snider, B.B.; Burbaum, Beverly Wolgast Synthetic Communications 1986, 16 (12), 1451-1460.

5. Intramolecular [2+2] Cycloaddition Reactions of Vinylketenes. Stereo- and Regiospecific Preparation of Vinylketenes from alpha, beta -Unsaturated Acid Chlorides. Kulkarni, Y.S.; Burbaum, Beverly Wolgast; Snider, B.B. Tetrahedron Lett. 1985, 26, 5619-5622.

6. Intramolecular Diels-Alder Reactions of Alkenylallenes. A Model Study for the Bottom Half of Chlorothricolide. Snider, B.B.; Burbaum, Beverly Wolgast J. Org. Chem. 1983, 48, 4370-4374.

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