TARGETING POSITION:

LABORATORY TECHNICIAN

Innovative and motivated biotechnologist with 3+ years of laboratory technician training as part of a comprehensive and hand-on Advanced Diploma program. Experienced with lab work in immunology, animal models, virology, mammalian cell culture, molecular biology, analytical chemistry and more with ability and curiosity to learn new techniques.

Spent up to 30 hours a week in lab environments: isolated and analysed DNA & RNA for molecular biology experiments including in-vitro protein expression, PCR and primer designing ; working with mice models for pathology and histology; prepared and processed samples; performed bio-hazardous sampling and waste management; implemented immunoassays including antibody staining, isolation and purification by magnetic separation, and ELISA; mammalian cell/tissue culture with aseptic techniques; working knowledge of flow cytometry; maintained and problem solved with lab instruments, and washed and autoclaved glassware; generated technical documentation and daily reports. Strong knowledge of aseptic techniques, cGLP, GMP, and safety procedures as well as well as its surroundings’ control check through environmental monitoring.

CORE SKILLS & COMPETENCIES

Biotechnologist

Analytical & Wet Chemistry

Virology

Molecular Biology

MS Office Suite

Food Sample Testing & Analysis

Class II Biological Safety Cabinet

Biosafety Level 2 labs

Sterile & Aseptic Techniques

LIMS

Tissue & Cell Cultures

SOP & Safety Procedures

Mathematical Calculations

Immunology & Serology

Bioinformatics Tools

ACADEMIC PROFILE

Advanced Diploma in Biotechnology Seneca College, Toronto, ON Jan 2013 – Apr 2016

President’s Honour List 2016

Mastered Skills & Concepts

Molecular Biology Cellular Biology Food Microbiology Animal Handling Lab Instrumentation Analytical &

Organic Chemistry Tissue/ Cell Culture Documentation & Technical Writing

DEVELOPMENT PROJECTS & LABORATORY WORK EXPERIENCE

1.Mice model manipulation Immunology & Anatomy

Goal: Demonstrated skills in handling, injecting, and blood withdrawal, tagging, breeding, and dissecting mice for immunology, tissue culture and histology studies.

Skills in Action: Carefully performed saphenous vein blood withdrawal, IV and subcutaneous injections. Aseptically isolated organs for the generation of primary cell line and histology slides.

Results: Successfully gained blood sample for serology testing and generated heart and liver cell line aseptically.

2.Isolation of plasmid pGFP from E.coli and digestion by Restriction Enzyme Molecular Biology

Goal: Isolation of the plasmid to cut it at certain point to create a recombinant DNA and analyze it by in- vitro expression of Green fluorescence protein into strains of E.coli which lack this protein.

Skills in Action: Isolated the plasmid from the bacterial culture by alkaline lysis causing pH change to open up the cell. Organic compound used in the experiment was Toluene. Plasmid was digested by HindIII enzyme and then amplified via PCR technique to get more copies for future experiments including recombinant protein expression. Each plasmid uncut and cut were analysed through gel electrophoresis i.e. SDS-PAGE gel.

Results: pGFPuv was expressed in E.coli when observed under UV light with the expression marker of ampicillin resistant gene.

3.Improvisation of Plaque Assay Virology

Goal: Challenged to work in teams with to increase plaque visibility by changing protocol points of nutrients.

Skills in Action: Independently conceptualized and strategized experiment guidelines. Developed a workflow chart, and prepared and used plate lysate (over liquid lysate) to manipulate Maltose and Magnesium Sulfate concentrations. Employed controls to confirm the validity of the assay.

Results: Successfully increased visibility of plaque, as nutrients with higher concentrations led to accelerated viral growth and, subsequently, to bulched or larger plaque.

4.Tissue Cultures/Cell Culture of Baby Hamster Kidney cells (BHK) Tissue/Cell Culture

Goal: Demonstrated ability to generate cell line subculture by enrichment, isolation and proliferation experiments to harvest it sterically/aseptically in Laminar Flow Hood Cabinets for the purpose of antibody testing, drug testing, and optimum serum level testing.

Skills in Action: Generated and maintained cell lines from the heart, liver, eyes, brain, kidney, and muscle cells of a chicken embryo. Conducted cryopreservation of cells in liquid nitrogen.

Results: Effectively prevented contamination in biological materials while successfully generating cell lines.

5.Food Sample Testing Microbiology

Goal: Directed to investigate and identify concentrations of disease causing microbes in food samples in accordance with Health Canada’s Product Safety Testing standards and FDA regulations. Test expiry dates on dairy products. And assess water samples for fecal and non-fecal coliform bacteria.

Skills in Action: Utilized biochemical methods in a level 2 Lab with a Biosafety cabinet class 2, Petrifilm™ and API20™, to detect coliforms and unknown bacteria or molds. Analyzed every product sample by serial dilutions that involved basic mathematical calculations and the use of analytical weighing instruments. Performed tests in an enclosed and ventilated workspace for working with materials potentially contaminated with pathogens.

Results: Discovered commonly sold food samples with elevated levels of microbes and reported them to Health Canada. Also, created consumer instructions to further reduce bacteria for products within the safe limits (washing, cooking, and temperature specifications).

6.Determination of Unknown Organic and Inorganic Compounds Analytical & Wet Chemistry

Goal: Showcased skillset to determine an unknown compound’s structure, as well as chemical and physical properties.

Skills in Action: Prepared reagents and solutions and used instruments including, UV-Vis spectrometry, FT-IR, HPLC, Gas chromatography, Mass chromatography, NMR to assess structure and polarity. Also used melting and boiling points to determine physical properties.

Results: Accurately determined unknown compounds and differentiated them into organic or inorganic category. And further classified them according to their properties.

WORK EXPERIENCE

Security Guard GardaWorld July 2014 - Present

Providing patrol, surveillance and CCTV monitoring services at various sites.

Tutor - Science & Math Bright Future Academy Sept 2013 – Apr 2016

Taught science and math to both high school and university level students.

Tutor & Mentor (Volunteer) Seneca College Mentoring Program Jan 2013 – Apr 2016

Employed creative teaching strategies to assist students with learning disabilities reach their academic goals.

CERTIFICATIONS & TRAINING

Bioethics Certification – TCPS Core 2 Panel of Research & Education, Government of Canada Nov 2015

CPR/ First Aid Certification GF1 Security Company July 2014

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