*

Gene

Gene (Guangjin) Li

*** *** ***** ***, ******** View, CA 94040

Email: acxgkn@r.postjobfree.com Tel.: 650-***-****

QUALIFICATION SUMMARY

Ph.D. specializing in immunology

Highly motivated, creative and versatile immunologist and molecular biologist

Extensive experience in human and murine cellular and immunological assays (immune cell

isolation, activation, proliferation, cytotoxicity, cytokine profiling, differentiation, signal

transduction, etc.), and DNA/RNA biology

Strong problem solving skills, independent and team, and multi-tasking worker.

TECHNIQUES

Mammalian cell culture, human and murine immune cell (PBMCs, T cells, B cells, NK cells,

monocytes) isolation, purification (AutoMACS, RosetteSep, EasySep) and culture, multicolor

flow cytometry, PhosFlow, intracellular cytokine staining and detection, ELISA, Luminex

assay, Western blotting, FACS, DC differentiation and DC-T cell coculture, T cell

proliferation (3H thymidine incorporation assay and CFSE labeling), cytotoxicity assays, Treg

suppression, antibody binding assay, mixed lymphocyte reaction, immunoprecipitation, cell

apoptosis assay, T cell differentiation (Th1, Th2, Th9, Th17, Treg)

DNA and RNA isolation and purification, PCR, cDNA synthesis, real-time PCR, primer

design, gene cloning, vector construction and plasmid preparation, recombinant protein

production in mammalian cells and Escherichia coli, protein purification, site mutation,

siRNA interference, mammalian cell transfection/nucleofection and reporter gene assay,

library construction, chromatin immunoprecipitation (ChIP).

Laboratory mouse handling: injections (SC, IP, IV), blood collection, spleen and lymph node

isolation, immunizations, bone marrow harvest, etc.

Biological and statistical softwares: FACSDiva, FlowJo, BioEdit, GraphPad Prism.

PROFESSIONAL EXPERIENCE

Scientist, Department of Immuno-Oncology, Rigel Pharmaceuticals, Inc., South San

Francisco, CA 94080 11/2015-10/2016

Design, develop and execute cell-based immune checkpoint assays using human and mouse

primary immune cells and cell lines, including cytokine profiling, proliferation, cytotoxicity,

Treg inhibition, etc.

Test anti-tumor effects of immune check point inhibitors in mouse model

Establish stable cell line expressing immune checkpoints and perform binding assay

Research Associate, Division of Immunology and Rheumatology, Stanford University

School of Medicine/PAVIR, Palo Alto, CA 12/2009-10/2015

Designed and conducted studies of Immune-inflammatory functions of IL-9 and Pu.1 in

immunosenescence and patients with giant cell arteritis.

Investigated enhancement of human immune functions (signaling transduction, cell activation,

cytokine production and cell proliferation) by targeting miR-181a targets

Designed and performed studies of type I interferon-mediated Janus kinase-signal transducer

and activator of transcription (JAK-STAT) signaling pathway, human T-cell differentiation

2

Gene

(Th1, Th2, Th9, Th17 and Treg), apoptosis, cytokine profiling, and target gene expression.

Postdoctoral Fellow, Lowance Center for Human Immunology and Rheumatology,

Emory University, Atlanta, GA 10/2004- 11/2009

Designed and conducted examination of age-associated lymphocyte incompetence in healthy

individuals and patients with rheumatoid arthritis including signaling transduction, cell

activation and proliferation, and cytokine production.

Uncovered epigenetic regulation and transcriptional factor recruitment of killer

immunoglobulin-like receptor (KIR) gene in human T cell subpopulations

Postdoctoral Associate, University of Minnesota Medical School, Minneapolis, MN

11/2001- 09/2004

Explored nuclear receptor-mediated gene transcription control, histone modification,

chromatin remodeling, and transcriptional factor interaction.

EDUCATION

Fudan University, Shanghai, China 07/ 2001

Ph.D., Microbiology; Area of specialization: viral immunology

Jiangxi Medical College, Jiangxi, China 07/1993

M.S., Pathophysiology; Area of specialization: cancer immunotherapy

PATENTS

1. Goronzy JJ, Weyand CM, Li G, et al, Methods to enhance T-cell mediated immune response.

Pub. No.: US 2011/0281932A1

2. Zheng Z, Yan W, Li G, et al. Development and preparation of a genetically engineered

peptide vaccine against foot-and-mouth disease. State Intellectual Property Office of

China

(patent No. ZL 02137011.7)

3. Zheng Z, Yan W, Sun Li, Li G, et al. A gene construction containing calcitonin gene-related

peptide (CGRP) and its preparation. State Intellectual Property Office of China (patent No.

ZL97106570.5).

TEACHING EXPERIENCE

Mentor: Junior postdoctoral fellows and undergraduate school student Internship, Stanford

University, 2010-2015

Trained and supervised research in immunology and molecular biology

Mentor: Graduate student research rotations, Emory University, 2007-2009

Trained and supervised research in immunology and molecular biology

PROFESSIONAL SERVICES/AWARDS

Review journal papers for Virology Journal, Journal of Molecular Medicine, Journal of

Biotechnology, Biochemical Pharmacology, and Experimental Gerontology

Granted 2006 Technology Invention Award (second place) by the Ministry of Education of

China for the invention of a FMD vaccine

3

Gene

SELECTED PUBLICATIONS

1. Li G, et al. Age-associated Failure to Adjust Type I Interferon Receptor Signaling

Thresholds after T-cell Activation. The Journal of Immunology, 2015; 195(3):865-74

2. Goronzy JJ, Li G, et al. The Janus head of T cell aging - autoimmunity and immune-

deficiency. Frontiers in Immunology, 2013; 4(131):1-10

3. Li G, et al. Decline in miR-181a expression with age impairs T cell receptor sensitivity by

increasing DUSP6 activity. Nature Medicine, 2012; 18(10):1518-24

4. Goronzy JJ, Li G, et al. Signaling pathways in aged T cells A reflection of T cell

differentiation, cell senescence and host environment. Seminars in Immunology, 2012;

24(5):365-372

5. Singh K, Deshpande P, Li G, et al. Increased B-Raf/K-Ras Expression in Rheumatoid

Arthritis (RA) T cells. Proceedings of the National Academy of Sciences, 2012; 109(25):

E1629-37

6. Yu M, Li G, et al. Signal inhibition by the dual-specific phosphatase 4 impairs T cell-

dependent B cell responses with age. Proceedings of the National Academy of Sciences,

2012; 109(15):E879-88

7. Li G, et al. Epigenetic regulation of killer immunoglobulin-like receptor expression in T

cells. Blood. 2009; 114(16):3422-30

8. Goronzy JJ, Li G, Weyand CM. DNA Methylation, Age-related Immune Defects, and

Autoimmunity, in Epigenetics of Aging, Ed. by Trygve O. Tollefsbol, Springer,2009, Nov.

p.327-44

9. Li G, et al. Epigenetic mechanisms of age-dependent KIR2DL4 expression in T cells.

Journal of Leukocyte Biology, 2008; 84(3):824-34

10. Lee WW, Yang ZZ, Li G, et al. Unchecked CD70 expression on T cells lowers threshold for

T cell activation in rheumatoid arthritis. Journal of Immunology, 2007; 179(4):2609-15

11. Naylor K, Li G, et al. The influence of age on T cell generation and TCR diversity.

Journal

of Immunology, 2005; 174: 7446-7452

12. Park SW, Li G, et al. Thyroid hormone induced juxtaposition of regulatory

elements/factors

and chromatin remodeling of Crabp1 dependent on MED1/TRAP220. Molecular Cell,

2005;

19(5): 643-653

13. Li G, et al. Comparison of immune responses against foot-and-mouth disease virus induced

by fusion proteins using the swine IgG heavy chain constant region or -galactosidase as a

carrier of immunogenic epitopes. Virology, 2004; 328 (2): 274-281

14. Li G, et al. Identification of histone deacetylase-3 domains that interact with the orphan

nuclear receptor TR2. Biochemical and Biophysical Research Communications, 2003; 31(2):

384-390

15. Franco PJ, Li G, et al. Interaction of nuclear receptor zinc finger DNA binding domains with

histone deacetylase. Molecular and Cellular Endocrinology, 2003; 206(1-2): 1-12

4

Gene

16. Li G, et al. CpG DNA enhances immune responses elicited by the DNA vaccine against foot-

and-mouth disease virus in guinea pigs. Chinese Science Bulletin (English edition), 2001; 40:

1376-1379

Contact this candidate