NIRMALA D NAG

**** * ******* ******, *** # *, Hinsdale, IL 60521 C: 773-***-**** acxbcc@r.postjobfree.com

PROFESSIONAL OVERVIEW

Motivated MS graduate in Biology with a research experience and intensive training in Cell and Molecular

Biology techniques, research processes and procedures, Quality control and Quality assurance. Currently

seeking Research Associate/Research Scientist/Quality Technician position in Medical devices and

Pharmaceutical companies. Proven research and analysis skills with strong focus on Cell and Molecular

Biology and Biochemistry. Highly detail-oriented and results driven.

SUMMARY OF SKILLS

Data analysis and interpretation

Nucleic acid extraction

Excellent interpersonal and

qPCR, RT-PCR, Electrophoresis,

organizational skills and

DNA Sequencing, ELISA, FISH

attention to detail

Spectrophotometry, HPLC,GC

Computer literacy, proficiency in

SDS-PAGE, Western Blotting,

MS Office

Cell Culture, Protein isolation,

Knowledge of Quality controls

Gene expression and silencing

and Quality System

Excellent verbal and written

Quality Assurance Management

communications skills

Familiar with CAP and CLIA

Pharmacogenetic testing

regulations

Technical report writing

Familiar with CAPA and writing

Familiar with regulations of

exception report, SOP

regulatory agencies, GMP, GLP

EDUCATION

2012 Master of Science: Applied Zoology

Kuvempu University Shankaraghatta, Karnataka, India

GPA: 3.8/4

Courses Studied: Cytogenetics, Developmental Biology, Molecular Biology,

Biotechnology, Biochemistry and Biostatistics, Genomics and Proteomics,

Applied and Population Genetics, Genetic Engineering, Organic Chemistry,

Microbiology, Genetics and Immunology.

2008 Bachelor of Science: Biological Sciences

Bangalore University Tumkur, Karnataka, India

GPA: 2.8/4

Courses Studied: Chemistry (Biochemistry, Organic chemistry, Inorganic

chemistry and Physical Chemistry), Botany and Zoology (Anatomy, Physiology)

THESIS/DISSERTATION

MS (Applied Zoology) thesis: "Effects of Mitochondrial DNA Variations on the

Occurrence of Diabetes Mellitus in selected South Indian Population". In this work

the prevalence of mtDNA mutations; rRNA, tRNA, and ND1 gene mutations in DM

patients of selected south Indian population were investigated. The results showed

the substitution of A3384G (ND1) G3316A (ND1) and T3202C (rRNA) mutations.

PUBLICATIONS

Nirmala D. Nag Prevalence of Mitochondrial rRNA, tRNA, and ND1 gene mutations

in Diabetes Mellitus patients of selected South Indian Population Masters thesis

published by: Lambert Academic Publishing' (ISBN978-3-659- 29987-2)

PROFESSIONAL EXPERIENCE

11/09/2015- to date Associate Scientist 1: QA & QC

Abbott Molecular - Des Plaines, IL

Duties Assigned:

Inspection of products and documents for non-conformances

Knowledge of rules and regulations of regulatory agencies: FDA

Knowledge of CAPA and Non- Conformances

Generation of Certificate of Analysis

Document review & Incoming material inspection

01/2015 to 03/2015 Research Assistant

University of Illinois at Chicago (UIC) Chicago

Duties Assigned:

Maximized operational efficiency of laboratory by recording and

analyzing results of research.

Cell Culture, Protein isolation, and silencing: Isolated targeted protein

from cultured cells and silenced targeted protein to analyze its function

in the cell.

SDS-PAGE, Western Blotting and Bradford Protein Assay: Separated

targeted protein using SDS-PAGE, western blot used to detect targeted

protein and Bradford Protein Assay used to measure the concentration

of the targeted protein.

Separation and purification of proteins using HPLC

At UIC, I got an opportunity to get hands on experience on Cell culture,

maintaining cell lines, Isolation of proteins and Protein Assays.

06/2014 to 12/2014 Research Associate

University of Chicago Medical Center Chicago

Duties Assigned:

Isolation of nucleic acids: Isolated DNA for identifying the gene involved in

cancer and efficacy of anticancer drugs on targeted gene.

Gel and Capillary Electrophoresis: Electrophoresis is used to separate

targeted gene based on size of DNA.

RT-PCR, q-PCR: Used to amplify and quantify targeted gene. Sequencing:

Used to analyze targeted gene modification.

Working at CLIA certified and CAP accredited Molecular Diagnostics Lab got

me a chance to get hands on experience in Pharmacogenetic testing:

Identification of resistance mutations in BCR-abl gene

07/2010 to 08/2010 Volunteer

University of Chicago Medical Center Chicago

Duties Assigned:

Isolation of Nucleic Acids: Isolated mitochondrial DNA for the MS

research project "Effects of Mitochondrial DNA Variations on the

Occurrence of Diabetes Mellitus in selected South Indian Population".

Gel and Capillary Electrophoresis: Electrophoresis is used to separate

targeted gene based on size of DNA.

RT-PCR, q-PCR: Used to amplify and quantify targeted gene.

Sequencing: Used to analyze targeted gene modification.

At the University Of Chicago Medical Center, I got a chance to get a hands on

experience on molecular techniques such as DNA isolation, sequencing and PCR,

I used that skills to do my MS research project.

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