Juan C. Ramirez

*** ***** ******, ** ****, Waltham, MA 02453

(928) 266 – 4236 acxa3p@r.postjobfree.com linkedin.com/in/juancramirez95 Education

Brandeis University, Waltham, MA

Bachelor of Science in Biology, Chemistry Expected May 2017 GPA: 3.63, Dean's List

Certifications

Certified in Boston-Consortium Chemical Hygiene Training & Environmental Health and Safety Training Laboratory Techniques and Computer Skills

Competency in molecular biology techniques, including RT & Q PCR, Western blot, fluorescence microscopy, genetic modification, siRNA, next-generation sequencing, and DNA/RNA isolation

Experience in ex vivo and in vivo biochemical methods, including flow cytometry, ELISA, HPLC, histology & immunostaining, pharmacokinetics, and mammalian & bacterial culture

Adeptness in laboratory upkeep procedures, encompassing reagent preparation, biological sample maintenance, small model organism development, and assay design & optimization

Proficiency in Python, MATLAB, GraphPad Prism, ImageJ, STAR Biochem, Microsoft Office Suite, Windows/OS Laboratory Experience

Mair Laboratory, Harvard TH Chan School of Public Health, Boston, MA Feb 2016 – Present Undergraduate Researcher

Design, optimize, and execute biological assays aiming to correlate for a multitude of aging-related phenotypes in several genetic strains of C. elegans

Correlate findings of conducted memory-based assays and laboratory-wide research with potential biochemical pathways of aging and linked pathologies

Collaborated in the preparation of novel strains of C. elegans and confirm successful genomic transformation by executing a variety of molecular biology techniques 3Scan, San Francisco, CA May 2015 – Aug 2015

Biomedical Research Intern

Researched present laboratory inquiries regarding cancerous cell angiogenesis and surveyed pertinent literature through journal databases and open sources

Developed provisional biological assays and optimized them as necessary, incorporating constructive feedback in future renderings

Explored developing novel methods in tissue imaging by evaluating present limitations and assessing potentially applicable emergent technologies

Camillus Health Center, San Luis, AZ Aug 2011 – Aug 2013 Medical Laboratory Technician

Scrutinized incoming tissue and fluid samples to match with outgoing results, focusing on upholding patient confidentiality and appropriate result distribution

Dispatched results in a concise manner to physicians and nurses to facilitate patient care

Ensured a sanitary, safe, and efficient laboratory setting by cleaning surfaces and equipment, following recommended protocols and calibrating machinery as necessary Research Experience

Department of Genetics and Complex Diseases, Harvard University, Boston, MA Apr 2016 – Aug 2016 Undergraduate Research Project

Devised a research and assay plan with fellow lab members and involved faculty, incorporating previous individual findings into a central project

Developed a model for aging systems in C. elegans to account for depletion & overexpression of AMPK, altered mitochondrial dynamics, and their individual relationships to neuronal health

Assessed multiple methods of quantifying C. elegans neurological fitness via chemotaxis across multiple strains

Investigated the translational potential of findings, and presented work to interested parties so as to receive feedback to be incorporated in future experiments

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