Bioconjugation, DNA

Tsai-Chin (Donna) Wu

********@*****.***

Experience

Intern, UST Inc., Carlsbad, CA [Nov. 2016 ~ current]

• Develop and optimize nucleic acid amplification chemistry (emulsion PCR) and workflow.

• Establish process and quality control assays.

Postdoctoral Scholar, University of San Diego, San Diego [Sep. 2015~July. 2016]

• Developed multiple protocols to fluorescently label DNA, including Qdot labeling, Alexa-dUTP, and yoyo1.

• Extract 10kbp to 120kbpDNA from bacmid.

Postdoctoral Fellow, Academia Sinica, Taipei, Taiwan [Jul. 2014~Aug. 2015]

• Molecular cloned glycoprotein and constructed recombinant baculovirus.

• Cultured insect cells and infected with the recombinant virus to express target proteins on cell membranes.

• Purified the glycoprotein hemagglutin with affinity columns.

• Studied viral infection diseases (flu and Ebola) and current vaccine products.

Postdoctoral Associate, Marshall University, Huntington WV [Apr. 2013~May 2014]

• Conducted FRET (Fluorescence Resonance Energy Transfer) analysis.

• Imaged DNA origami with AFM and fluorescence microscopy, and analyze results with ImageJ and Matlab.

• Designed origami-based nanosensors and conduct single molecule imaging.

• Prototyped microfluidic chips that assemble origami automatically. Process includes photolithography and Labview programming for syringe pumps control.

• Applied super resolution techniques, include FIONA and 3B, on origami optical images.

Intern, Aptagen, LLC, Jacobus, PA [Aug. 2012 ~ Apr. 2013]

• Synthesized oligonucleotides with modifications, such as biotin, thiol, fluorophores and quenchers.

• Wrote SOP for experiment procedures and mandatory tasks.

• Interacted with customers for quests and questions.

Research Assistant, UIC Biotechnology Research Group, Chicago, IL [Aug.2008 ~ Apr.2012]

• Bioconjugated DNA/protein to biomarkers/fluorescent colloidal nanoparticles and developed diagnostics molecular beacons for water-based solution;

• Familiar with carboxyl-to-amine and thiol-to-maleimide bioconjugation processes;

• Developed potassium ion, Salmonella and Listeria monocytogen optical biosensors and tested them in vitro;

• Analyzed nanostructures using micro Raman spectroscopy, and photoluminescence;

Education

Ph.D., Dept. of Bioengineering / Nanotechnology, Chicago IL, US [Aug 2006 ~ Apr 2012]

Univ. of Illinois at Chicago (UIC)

B.S., Dept of Physics with Bioinformatics Certificate, Taipei, Taiwan [Sep 2002 ~ June 2006]

National Taiwan Normal University

Skills

Computer Softwares/Programming

Matlab, Labview, ImageJ, Java, Visual Basic, C#

Wet Chemistry Lab techniques

DNA origami assembling, Electrophoresis, Bioconjugations, Substrate (glass overslips) cleaning, lithography, Molecular cloning, Recombinant baculovirus expression system.

Professional Instruments operation

Protein purification (Affinity column)

Atomic Force Mircroscopy

Optical microscopy

Spectrofluorometry

Micro Raman spectrometer

Varian Evaporation system (Clean room

experiences)

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