Bolin, David A
** ********* **** ******, ** *****
************@*******.***
Objective: To obtain a position in a biopharmaceutical company.
Education: North Carolina Central University Fall, 2011-Fall, 2014
Bio-manufacturing Research Institute Technology Enterprise (B.R.I.T.E.)
Durham, North Carolina
Bachelors, Pharmaceutical Sciences, Minor Biology & Chemistry
Graduation Date: December, 2014
Cleveland Community College Fall, 2006-Spring 2011
Shelby, North Carolina
AAS, Biotechnology
Certification: HPLC Certification
North Carolina State University
Bio-manufacturing Training and Education Center (B.T.E.C)
Raleigh, North Carolina
Research Experience: Undergraduate Research Assistant
North Carolina Central University
Bio-manufacturing Research Institute Technology Enterprise (B.R.I.T.E.)
Principle Investigator: Dr. Kevin Williams
Performed Assays
Purified Protein
Transformed Bacteria
Performed Chemical Screens
Performed Restriction Digest
Performed Alkyline Lysis
Relevant Courses: General Physics I, Organic Chemistry I, Organic Chemistry I Lab,
Organic Chemistry II, Organic Chemistry II Lab, Biochemistry,
Basic Lab Techniques, Biostatistics, Molecular Biology,
Microbiology, Bioprocessing, Biochemistry I Lab, Biochemistry II,
Scientific Writing, Genetics, Immunology, Bio-analytical Chemistry,
Cell Culture, and Assay Design
Laboratory Techniques: Buffer Preparation, pH Measurement, Making Solutions, Sterile
Techniques, Record Keeping, Protein Assays, HPLC, FPLC, SDS PAGE,
UV/VIS, Western Blot, Light Microscopy, DNA/RNA Isolation, PCR,
Cell Transformation, E.L.I.S.A, Chemical Screens, Fluoroscopy, DNA Sequencing, Bioreactor assembly and maintenance, GC/MS RFID
Work Experience: QA/ QC R&D 07/18/2016 – Present
Horner International
Inspect products and packaging before shipment
Performed test measure to insure products are in specification
Designed new processes for proudction
Research Associate II 10/23/2015 – 06/18/2016
Sequris Inc.
Prepared Bioreactors for Flu vaccine in downstream process
Maintained external and internal conditions of bioreactor
Prepared equipment, buffers and media
Inactivate virus for lysis
Research Technician 03/02/2015-10/16/2015
Biomedical Biotechnology Research Institute
Principle Investigator: Dr. Mildred Pointer
• Sample preparations
• Inventory
• Assays
• Data compilation
Research Assistant 08/21/2012-05/16/2014
Biomedical Biotechnology Research Institute
North Carolina Central University
Principle Investigator: Dr. Kevin Williams
Transformed bacteria for mammalian protein production
Verified presence of protein, Purified protein
Laboratory Assistant 08/24/2007-06/24/2011
Department of Biotechnology
Cleveland Community College
Principle Investigator: Dr. Sandol Johnson
Prepared labs for class experiments
Chemical inventory
Purchasing and ordering
Reference upon Request
https://www.youtube.com/watch?v=AdTCgiL2rvY