Bolin, David A

** ********* **** ******, ** *****

984-***-****

acx2b0@r.postjobfree.com

Objective: To obtain a position in a biopharmaceutical company.

Education: North Carolina Central University Fall, 2011-Fall, 2014

Bio-manufacturing Research Institute Technology Enterprise (B.R.I.T.E.)

Durham, North Carolina

Bachelors, Pharmaceutical Sciences, Minor Biology & Chemistry

Graduation Date: December, 2014

Cleveland Community College Fall, 2006-Spring 2011

Shelby, North Carolina

AAS, Biotechnology

Certification: HPLC Certification

North Carolina State University

Bio-manufacturing Training and Education Center (B.T.E.C)

Raleigh, North Carolina

Research Experience: Undergraduate Research Assistant

North Carolina Central University

Bio-manufacturing Research Institute Technology Enterprise (B.R.I.T.E.)

Principle Investigator: Dr. Kevin Williams

Performed Assays

Purified Protein

Transformed Bacteria

Performed Chemical Screens

Performed Restriction Digest

Performed Alkyline Lysis

Relevant Courses: General Physics I, Organic Chemistry I, Organic Chemistry I Lab,

Organic Chemistry II, Organic Chemistry II Lab, Biochemistry,

Basic Lab Techniques, Biostatistics, Molecular Biology,

Microbiology, Bioprocessing, Biochemistry I Lab, Biochemistry II,

Scientific Writing, Genetics, Immunology, Bio-analytical Chemistry,

Cell Culture, and Assay Design

Laboratory Techniques: Buffer Preparation, pH Measurement, Making Solutions, Sterile

Techniques, Record Keeping, Protein Assays, HPLC, FPLC, SDS PAGE,

UV/VIS, Western Blot, Light Microscopy, DNA/RNA Isolation, PCR,

Cell Transformation, E.L.I.S.A, Chemical Screens, Fluoroscopy, DNA Sequencing, Bioreactor assembly and maintenance, GC/MS RFID

Work Experience: QA/ QC R&D 07/18/2016 – Present

Horner International

Inspect products and packaging before shipment

Performed test measure to insure products are in specification

Designed new processes for proudction

Research Associate II 10/23/2015 – 06/18/2016

Sequris Inc.

Prepared Bioreactors for Flu vaccine in downstream process

Maintained external and internal conditions of bioreactor

Prepared equipment, buffers and media

Inactivate virus for lysis

Research Technician 03/02/2015-10/16/2015

Biomedical Biotechnology Research Institute

Principle Investigator: Dr. Mildred Pointer

• Sample preparations

• Inventory

• Assays

• Data compilation

Research Assistant 08/21/2012-05/16/2014

Biomedical Biotechnology Research Institute

North Carolina Central University

Principle Investigator: Dr. Kevin Williams

Transformed bacteria for mammalian protein production

Verified presence of protein, Purified protein

Laboratory Assistant 08/24/2007-06/24/2011

Department of Biotechnology

Cleveland Community College

Principle Investigator: Dr. Sandol Johnson

Prepared labs for class experiments

Chemical inventory

Purchasing and ordering

Reference upon Request

https://www.youtube.com/watch?v=AdTCgiL2rvY

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