Pasquale Lasala

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Pasquale Lasala

acwxxb@r.postjobfree.com

via Lungomare C. Colombo, 325 • Salerno, Italy 84100 • +39-331-******* CURRENT POSITION

University of Sannio, DST Benevento, BN

• Research Fellow 2015-Present

• Well-being in an airplane fellowship, POR FSE Campania 2007/2013 EDUCATION

Second University of Naples (SUN), DiSTABiF Caserta, CE

• Ph.D. in Molecular and Cellular Biology December 2014

• Fellowship for a Ph.D. student program: Dottorati in Azienda POR FSE Campania 2007/2013 Second University of Naples (SUN), DiSTABiF Caserta, CE

• M.S., Biology 2011

Second University of Naples (SUN), DiSTABiF Caserta, CE

• B.S., Biotechnology 2008

RESEARCH/WORK EXPERIENCE

University of Sannio, DST Benevento, BN

Research Fellow with Prof. Fernando Goglia 2015-Present Well-being in an airplane, metabolism and oxidative stress.

• Measure of metabolic rate, using indirect calorimetry in order to assess energy expenditure and also the oxidation of energy substrates using CLAMS Oxymax System.

• Analysed serum levels of oxidative stress markers, 8-oxo-dG. Second University of Naples (SUN), DiSTABiF Caserta, CE Graduate Researcher with Prof. Antonia Lanni 2011-2014 Effect of 3,5,3'-triiodothyronine and 3,5-diiodothyronine on mitochondrial DNA damage and repair.

• Examined effects of thyroid hormones on mitochondrial DNA integrity.

• Executed Western Blot and real-time PCR analysis to study genes involved in mtDNA repair.

• Executed ELISA assay to characterize serum levels of 8-oxo-dG. Leibniz Institute for Age Research, (FLI) Jena (Germany) Visiting Ph.D. Student with Dr. Heike Heuer May-August 2014 Mitochondrial function of the thyroid metabolite T2 and its implication in diet-induced obesity.

• Examined metabolic rate using Oxymax Lab Animal Monitoring System (CLAMS).

• Examined metabolic effects of thyroid hormones using Western Blot and PCR analysis. Tecno Bios Laboratories, Srl Apollosa, BN

Ph.D. Student Intern (Stage) 2012-2014

• Performed Western Blot and PCR analysis.

• H.A.C.C.P.

• Evaluation of occupational hazards for workers. For example, to chemical risk or biological risk.

• Managed laboratory functions including organization. Second University of Naples (SUN), DiSTABiF Caserta, CE Graduate Intern with Dr. Rosalba Senese 2010-2011

• Examined metabolic effects of thyroid hormones using Western Blot and PCR analysis.

• Managed laboratory functions including organization, ordering and scheduling equipment use. Second University of Naples (SUN), Department of Experimental Medicine Naples, NA Graduate Student with Prof. Chiara Schiraldi 2007-2008 Pasquale Lasala

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Streptococcal fermentation to produce hyaluronanacid.

• Cultured aerobic, operated fermentation processes under aerobic conditions.

• Executed bacterial fermentations in different scales, operation modes (batch and fed-batch modes) to improve production of desired products.

• Optimization of fermentation conditions.

SKILLS and TECHNIQUES

• In-vivo handling (mouse and rat) (e.g. hypothyroidism model)

• Ex-vivo (e.g. hypothalamic explants)

• Isolation of RNA and DNA

• Cell culture

• PCR, RT-PCR and quantitative PCR

• Oxymax Lab Animal Monitoring System (CLAMS)

• ELISA Assay

• Western Blot

• Quant-iT PicoGreen dsDNA Assay

• BATCH e FED-BATCH fermentation processes

Software: MS Office, internet research. Experience with Photoshop. Languages: Mother tongue in Italian and conversational English. Interests: Swimming, Traveling, Wine.

Other: Diploma AIS (Italian Sommelier Association), Lifeguard Certification (Federazione Italiana Nuoto). LEADERSHIP EXPERIENCE

Second University of Naples (SUN), DiSTABiF Caserta, CE Teaching Assistant Physiology 2014

• Assisted in preparation and execution of experiments.

• Prepared and presented 2 class lectures.

Second University of Naples (SUN), DiSTABiF Caserta, CE Mentor, Mentoring for Undergraduate Student in Biology 2011, 2013

• Guided undergraduate students to understanding of scientific method through molecular biology experiments and case based learning.

Co Founder: Stanze sul Mare, B&B Salerno, SA

Recruited and currently managing a small B&B staff. January 2000-Present PUBLICATIONS

• Cioffi F., Senese R., Lasala P., Ziello A.,Mazzoli A., Crescenzo R., Liverini G., Lanni A., Goglia F., Iossa S. (2015) Fructose-rich diet affect mitochondrial DNA damage and repair in rats. Submitted.

• Senese R., Lasala P., Leanza C., de Lange P. (2014) New avenues for regulation of lipid metabolism by THs and analogs. Front. Physiol. doi: 10.3389/fphys.2014.00475.

• E. Silvestri, M. Coppola, A. Ziello, P. Lasala, C. Leanza, M. Moreno. Insight on the Body Fat Lowering Effect of 3,5-diiodo-L-thyronine. Immun., Endoc. & Metab. Agents in Med. Chem., 2013, 13, pp. 159-164(6).

CONFERENCE

Pasquale Lasala

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• Cioffi F., Lasala P., Ziello A., Innucci L., Giacco A., Goglia F. and Lanni A. The effect of 3,5-diodo-L- thyronine and 3,5,3'-triodio-L-thyronine on mitochondrial DNA integrity. 15th International Thyroid Congress (ITC), Orlando, Florida (USA) 18-23 Ottobre 2015.

• Cioffi F., Senese R., Ziello A., Lasala P., Mazzoli a., Crescenzo R., Liverini G., Iossa S., Lanni A. and Goglia F. The effect of fructose-rich diet on mtDNA oxidative stress. 66th National Congress of the Italian Physiological Society, Genoa (Italy), 16-18 Settembre 2015.

• R.A. Busiello, S. Savarese, M. Coppola, C. Leanza, P. Lasala, A. Ziello, D. Glinni, F. Goglia. Metabolic adaptation induced by triiodothyronine: a role for uncopling protein 3. 64th National Congress of the Italian Physiological Society, Portonova (Italy) Settembre 2013.

• R. Senese, P. De Lange, C. Leanza, A. Ziello, P. Lasala, R. De Matteis, D. Lattanzi, A. Lanni. Effect of 3,5-diiodo-L-thyronine on hight-fat diet-induced visceral adiposity in the rat. 37th Annual Meeting of the European Thyroid Association, Leiden (Holland), 7-11 Settembre 2013.

• Lasala P., Leanza C., Ziello A., De Lange P., Lanni A., Cioffi F.Physiological mtDNA repair is decreased in liver of high fat diet rat. Giornate Scientifiche di Ateneo (SUN), Caserta (Italy), 26-27 Giugno 2013.

• P. Lasala, C. Leanza, A. Ziello, P. De Lange, A. Lanni, F. Cioffi. Impact of high fat diet on mitochondrial DNA damage and repair in rat liver. Annual Meeting of Young Research Meeting, Anacapri (Italy) 21-24 Maggio 2013.

• R. Senese, C. Leanza, P. Lasala, A. Ziello, P. De Lange, A. Lanni. 3,5-diiodo-L-thyronine improves mitochondrial function in nonalcoholic steatosis through the stimulation of SIRT3 mitochondrial targets. 63 National Congress of the Italian Physiological Society, Verona (Italy), 21-23 Settembre 2012.

• P. Lasala, C. Leanza, A. Ziello, F. Cioffi,, A. Lanni. The phenotipic/metabolic response of skeletal musle of high-fat diet fed overweight rats to TRC150094 treatment. Giornate Scientifiche di Ateneo

(SUN), Caserta (Italy), 10-11 luglio 2012.

• C. Leanza, P. Lasala, A. Ziello, R. Senese, P. De Lange, A. Lanni.Prevention of diet-induced insulin resistance by 3,5-diiodo-L-thyronine in rats involves SIRT1. Giornate Scientifiche di Ateneo (SUN), Caserta (Italy), 10-11 luglio 2012.

• Leanza C., Lasala P., Ziello A., Senese R., De Lange P., Lanni A.. 3,5-diiodo-L-thyronine (T2) prevents diet-induced adiposity by activating hepatic SIRT1 and SIRT3. Giornate Scientifiche di Ateneo (SUN), Caserta (Italy), 26-27 giugno 2013.

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