Yuji Takeda

Boston, MA ***** 781-***-****

acwurf@r.postjobfree.com https://sites.google.com/site/yujistakeda/ 1

Education

PhD in Biomedical Engineering Tufts University, Medford, MA May 2016 MS in Mechanical Engineering and Science Kyoto University, Kyoto, Japan Mar 2011 BS in Engineering Kyoto University, Kyoto, Japan Mar 2009 Research experience

Postdoctoral Research Fellow Brigham and Women’s Hospital, Boston, MA Jun 2016–present Research Fellow Harvard Medical School, Boston, MA Jun 2016–present PI: Jakub Godlewski

Measured microRNA transfer via exosomes between glioma stem cells Research assistant Tufts University, Medford, MA Sep 2011–Mar 2016 PI: Qiaobing Xu

Demonstrated that exosomes from neuronal cells can differentiate mesenchymal stem cells (MSCs) into neuron-like cells

Identified proteins contained in exosomes using LC–MS

Determined the optimal chemical structure of synthetic lipid nanoparticles (lipidoids) for gene delivery by performing screening assays

Delivered miRNA to MSCs using lipidoids and evaluated neuronal differentiation of MSCs

Developed micropatterned 2D and 3D scaffolds made of decellularized extracellular matrix by replica molding and lyophilization

Developed experimental protocols for the laboratory Research assistant Kyoto University, Kyoto, Japan Apr 2008–Mar 2011 PI: Naohide Tomita

Developed software to quantify intercellular interaction and migration of chondrocytes on silk surfaces

Measured adhesion force and cell spreading area of chondrocytes on various silk surfaces Intern Department of Primary Industries (Victoria), Victoria, Australia Jun 2010–Aug 2010

Treated myoblast cell line (C2C12) with different protein samples

Evaluated protein expression in cells using ELISA and LC–MS Practical skills

Cell-based assays: cell culture (glioma stem cells, neurons, astrocytes, MSCs, NSCs, endothelial cells, chondocytes), stem cell differentiation (osteoblast, adipocyte, chondrocyte, neuron), exosome isolation, qPCR, Western blot, cell viability assay, ELISA, 3D cell culture, flow cytometry, cell sorting, endothelial cell tube formation assay, cell migration assays, transfection (liposome, lentivirus), bacterial transformation using E. Coli Microscopy: SEM, confocal microscopy, immunofluorescence microscopy Material analysis: DLS, ultracentrifugation, lyophilization, replica molding, LC–MS, tensile testing (Instron) Analysis Software: ImageJ, GIMP, ChemDraw, Cell Quest Pro, MxPro Programming: C

Languages English, Japanese

Awards

Fellowship, Keidanren Ishizaka Memorial Foundation Sep 2011–Aug 2013 Graduate Student Travel Fund, Tufts University Dec 2012 Travel Grant, Wakisaka Foundation Sep 2010

Yuji Takeda

Boston, MA 02130 781-***-****

acwurf@r.postjobfree.com https://sites.google.com/site/yujistakeda/ 2

Publications

1. Takeda YS, Wang M, Deng P, Xu Q. Synthetic Bioreducible Lipid-based Nanoparticles for miRNA Delivery to Mesenchymal Stem Cells to Induce Neuronal Differentiation, Bioeng Transl Med (2016) in press 2. Wang M, Altinoglu S, Takeda YS, Xu Q. Integrating Protein Engineering and Bioorthogonal Click Conjugation for Extracellular Vesicle Modulation and Intracellular Delivery, PLoS One 10 (2015) e0141860. 3. Takeda YS, Xu Q. Neuronal Differentiation of Human Mesenchymal Stem Cells Using Exosomes Derived from Differentiating Neuronal Cells, PLoS One 10 (2015) e0135111. 4. Takeda YS, Xu Q. Synthetic and Nature-derived Lipid Nanoparticles for Neural Regeneration, Neural Regen Res 10 (2015) 689-690.

5. Takeda YS, Xu Q. Fabrication of 2D and 3D constructs from reconstituted decellularized tissue extracellular matrices, J Biomed Nanotechnol 10-201*-****-****. 6. Otaka A, Takahashi K, Takeda YS, Kambe Y, Kuwana Y, Tamada Y, Tomita N. The distance and duration of cell co-migration during cell aggregation on fibroin substrates, Tissue Eng Part C Methods 20 (2014) 671- 680.

7. Hashimoto T, Kojima K, Otaka A, Takeda YS, Tomita N, Tamada Y. Quantitative evaluation of fibroblast migration on silk fibroin surface and TGFBI gene expression, J Biomater Sci Polym Eds 24 (2013) 158–169. 8. Kambe Y, Takeda Y, Yamamoto K, Kojima K, Tamada Y, Tomita N. Effect of RGDS-Expressing-Fibroin Dose on Initial Adhesive Force of a Single Chondrocyte, Biomed Mater Eng 21 (2010) 309–316. Presentations

1. Koo D, Zhao X, Takeda YS, Xu Q. Proteomic Analysis of Exosomes Derived from Neuronal Cells to Determine Factors Promoting Neuronal Differentiation, Biomedical Engineering Society Annual Meeting

(2016)

2. Zhao X, Takeda YS, Xu Q. Proteomics and miRNA Analysis of Exosomes Derived from Differentiating Neuronal Cells, 5th Annual Meeting of the International Society for Extracellular Vesicles (2016) 3. Takeda YS, Xu Q. Decellularized Tissue Extracellular Matrices: a Potential Source of Biomaterials for Tissue Engineering, the Society For Biomaterials 2013 Annual Meeting and Exposition (2013) 4. Takeda YS, Otaka A, Tamada Y, Tomita N. Quantification of the relationship between cell shape and intercellular adhesion of chondrocytes on a fibroin substrate, 23rd European Conference on Biomaterials (2010)

5. Otaka A, Shimada Y, Kawakami M, Takeda Y, Kachi ND, Yamamoto K, Tamada Y, Suguro T, Tomita N. Quantitative evaluation of cell aggregating process at early stage of cartilage regeneration, 56th Annual Meeting of the Orthopaedic Research Society (2010) Teaching experience

Teaching assistant Tufts University, Medford, MA Sep 2013–Dec 2013 BME250 (Principle of Biomedical Engineering)

Prepared presentation and discussed tissue engineering, drug delivery, and commercialization at class

Prepared and graded quizzes

Assisted and evaluated research-based term paper Professional activities

Kagakusha Network, http://www.kagakusha.net Jan 2012–present President (2015–present), Vice president (2013–2014) Online group of 200+ Japanese researchers and students studying outside of Japan

Organized a career planning webinar for graduate students

Advised undergraduate members preparing their graduate school applications

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