James F. Wick

**** ****** ****** *******

Franklin, Wisconsin 53132

414-***-**** (home)

414-***-**** (cell)

E-Mail: acwse6@r.postjobfree.com

SUMMARY

Molecular Biologist with extensive hands-on laboratory experience. Proven record of accomplishment in research, product development, operations management, and quality assurance. Effective planner, designer, and implementer of quality systems across business functional lines. Administered regulatory issues with state, federal, and private agencies including the FDA, EPA, NRC, OSHA, and DNR. Accomplished at process design and development involving fermentation, protein/enzyme separation, and purification techniques. Devised assay procedures and methods for developing and improving reagent manufacturing. Routinely performs standard molecular biology techniques including DNA and RNA extractions, nucleic acid manipulation, ELISA, and electrophoresis. Excellent experimental design and data analysis skills. Leader in research, business development, and technical marketing. Excellent writing, teaching, training, and mentoring skills. Holds Masters degrees in both Biochemistry and Business Administration.

CORE COMPETENCIES

Quality Assurance/Quality Control Molecular Biology/Microbiology Techniques

New Product Development Marketing & Sales

Laboratory Start-up/Division Growth Operations Management

Strategic Planning Legal/Technical Liaison

Customer Sales/Service Radiation safety

CAREER PROFILE

Laboratory Technician Dec. 2014-Present

Pentair

Glendale, WI

Nissen Staffing contractor performing quality assurance testing of point-of-use drinking water faucet filters. Water sampling for lead and total trihalomethane (TTHM), along with parameter tests for pH, turbidity, total dissolved solids (TDS), alkalinity, hardness, and chlorine. Analysis conforms to NSF /ANSI 42, 53, and 401 residential drinking water testing procedures.

Auto Reconditioning Sales Aug. 2013 - April 2014

Russ Darrow Group

Milwaukee, WI

Sold used car reconditioning services (paint-less dent repair, mobile paint touch-up and body shop repairs) within the Russ Darrow auto dealerships. This was a pilot program developed to internalize these services. Responsible for covering the 19 Russ Darrow dealerships throughout eastern Wisconsin.

Consultant, Document Review Dec. 2010- Jan. 2013

Hudson

Chicago, IL

Contract position reviewing sensitive, confidential foreclosure documents on behalf of a major financial institution located in Milwaukee, Wisconsin. Coordinated individual reviews with corporate staff, corporate legal counsel, and when necessary outside foreclosure counsel. Performed quality control functions concerning other reviewers work product. Provided documentation for Office of Currency and Comptroller (OCC) directed audit. Mentored/trained additional review teams. Trained to research foreclosure customer complaints and suggest remediation steps.

Franchisee/ Operations Director 2002- 2010

Maaco Collision Repair & Auto Painting

Menomonee Falls, WI

A franchise owner responsible for entire shop’s operations including strategic planning, sales and marketing, staff development, purchasing and inventory control, operations, and financial performance. Auto collision repair estimator and lead sales person. Managed the scheduling of repairs and painting of customer vehicles. Instituted quality procedures that resulted in paint and body shop certification through Maaco Corporate. Shop’s Consumer Survey Index (CSI) scored greater than corporate average. Increased shop’s weekly car throughput and business revenue. 12-employee shop with revenue of $750,000. Business sold October 2010.

Scientist; Molecular Biologist 1985-2000

Molecular Biology Resources, Inc., Milwaukee, Wisconsin

Scientist and Technical Services Manager for start-up biotechnology company that specialized in OEM of DNA and RNA modifying enzymes, and restriction endonucleases. Produced/purified company’s first enzyme product-line (approximately 50 products). Led development team to introduce new molecular biology products and kits. Prepared organization’s first retail catalog of molecular biology reagents. Designed and implemented a comprehensive quality control system patterned after ISO 9000 and GMP guidelines. Acquired Nuclear Regulatory Commission license for use of radioisotopes and served as company’s Radiation Safety Officer. Prepared and conducted the corporate Hazardous Communications Program. Managed the organization’s system for dealing with customer’s technical inquires. Served as technical liaison to corporate legal counsel regarding contracts, intellectual property, and patent applications. Developed a patented nucleic acid amplification and detection system (US Patent 6,063,604).

Purified and performed quality control testing on an extensive line of DNA and RNA modifying enzymes. Accomplished at aseptic technique, fermentation, enzyme purification, process and assay development, product and process quality control, product release to finished goods inventory, and record keeping, DNA and RNA extractions, nucleic acid manipulation, electrophoresis, and data analysis. Conducted large-scale pilot-plant protein purification and 250L fermentation. Extensive experience using numerous chromatography resins (ion exchange, affinity, antibody, hydrophobic, gel-filtration) and other protein isolation and characterization methods. Experienced with FPLC and Phast-gel systems.

EXPERIENCE AND ACHIEVEMENTS

Product Development

Purified and introduced MBR’s first line of molecular biology grade modifying enzymes.

Introduced numerous new products, including the first commercial source of Taq DNA Polymerase used in PCR and RT-PCR.

Produced commercial and research batches of other thermophilic DNA polymerases (Tfl, Tth, Tru, Bst DNA Polymerase) along with thermophilic DNA ligase and pyrophosphatase.

Assisted in developing and improving manufacturing processes and products

Expert at protein purification, upstream processing, and technology transfer.

Experienced at handling pathogenic microorganisms and viruses.

Operations/Project Management

Lead teams of researchers in the area of new product development.

Developed a patented nucleic acid amplification and colorimetric target detection procedure (Target Nucleic Acid Sequence Amplification, US Patent 6,063,604).

Developed OEM component for FDA approved medical IVD kit sold by a major diagnostic/pharmaceutical company.

Performed pilot plant/large-scale fermentations and enzyme/protein purification.

Quality Assurance/Quality Control

Designed and implemented a comprehensive QA/QC program that encompassed document control, development/maintenance of batch production records, SOP writing, product part-specifications, establishment of criteria for acceptance of materials into finished goods inventory, and equipment maintenance and calibration schedules and procedures.

Responsible for internal audits of the corporate quality system and representation of the quality system to external auditors/customers.

Managed performance and validation studies

Managed product stability testing program.

Experienced at GMP/GLP practices.

Technical Services

Managed customer technical inquiries.

Trouble shot customer technical problems.

Conducted investigations regarding customer complaints.

Planned and conducted corporate R&D meetings.

Represented corporate at trades shows, conferences, and seminars.

Prepared and administered the company’s Hazardous Communications Program.

Acquired Nuclear Regulatory Commission licenses for the use of radioisotopes (C14, H3, P32, S35).

Served as the corporate Radiation Safety Officer.

Managed twenty-five radioisotope users in a radiation program serving three locations.

Acquired kosher certification (Circle-K) for food product production at MBR’s Wisconsin BioProducts division.

Proficient in the use of Microsoft Office, Project Management, and Audatex.

Monitored scientific literature, trade journals, and events using numerous searching tools.

Laboratory Techniques

Nucleic acid amplification: PCR, RT-PCR, NASBA, SDA, RAMP

DNA and RNA isolation, plasmid purification

Protein purification, extractions, chromatography, SDS-PAGE, FPLC, upstream processing

Basic cloning techniques

Immunology: ELISA, Western blots

Sequencing

Autoradiography

Aseptic and basic Microbiology Methods

Media Prep and Fermentation

Legal/ Marketing

Worked with legal counsel on structuring inter-business confidentiality agreements, proprietary information issues, and drafting patents.

Proficient at doing patent searches and monitoring court legal proceedings.

Developed marketing literature and advertisements.

Prepared Company’s first retail product catalog.

Engaged in marketing functions and technical support at scientific trade shows.

Presented poster sessions and seminars at scientific meetings.

EDUCATION

Masters of Science

Medical College of Wisconsin

Milwaukee, Wisconsin,

Biochemistry

Masters of Business Administration

Cardinal Stritch College

Milwaukee, Wisconsin

General Management

Bachelors of Science

Carroll College

Waukesha, Wisconsin

Biology & Chemistry

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