VIDYARANI NANAPUR

Mobile: 998-***-****

E-mail: acwopr@r.postjobfree.com

OBJECTIVE

Overall four years experience as a Microbiologist with extensive skills in diagnostic and pharmaceutical microbiology. Seeking position as a QC Microbiologist at Dr.Reddy's Laboratories utilizing extensive experience and skills in laboratory techniques and equipment usage since 6th April 2015-till date

KEY RESPONSIBILITIES

To Follow CGMP, CGLP, CGDP, and safety measures as per the given guidance.

Preparation of Disinfectants, recording of temperatures and Relative Humidity in microbiological lab.

To Perform TOC and microbiological sampling of water samples as per schedules.

Maintenance of Microbiological Cultures (Bio Balls).

Maintenance of media Management.

To ensure proper usage and document the Media stock. Media consumption, bio ball stock, bio ball consumption details used for routine Microbiology Analysis.

Preparation and Performance of Growth promotion, sterility and Growth indicative properties of different Microbiological Medias.

Performing TOC analysis of all units for water samples.

To ensure proper handling of Autoclaves, Performance verification of Analytical Balances, pH meter.

Performing periodic Environmental Monitoring of Clean rooms and Microbiology Laboratory as per the schedule.

To ensure proper usage of Buffers within the validity period.

To ensure appropriate House Keeping in API microbiology lab.

To prepare the Incubator temperature monitoring reports from ICRDAS 1.2 Version.

To ensure proper labeling of Performance cheek labels of Analytical Balance, pH meter.

To ensure proper cleanliness of Area.

To ensure proper positioning of spirit level, Cleanliness of Balance.

To perform Gram staining techniques.

To subculture the isolates of investigation sample.

Performing periodic Environmental Monitoring of Clean rooms and Microbiology Laboratory as per the schedule.

Performing Microbiological sampling and analytical of water like purified water and potable water.

Release of water sample results and TOC results as per timelines.

To report immediately to the supervisor for any incidents/ spillages observed during the weighing of Media.

To report immediately to the supervisor for any OOS/incidents observed during the analytical.

Responsible for maintaining the clean room behaviors and to follow aseptic techniques during Microbiology Analysis.

Shall undergo the functional trainings.

To ensure the shift work is handed over appropriately to reliever before leaving the lab.

Following the measures as per the guidance.

PPE shall be used at work place.

Media Management

Preparation and maintenance of media.

To perform Gram staining techniques, Isolation identification of micro organisms.

Performing Microbiological sampling of water like purified water, potable water etc.,.

Performing Microbiological analysis of water.

Release of water sample results.

Isolation of identification of microorganisms

Maintenance of isolates.

Maintain accurate log books of all performed procedures.

WORK EXPERIENCE

Worked as Jr.Technologist - Microbiology & Serology at American Institute of Pathology and Laboratory Sciences from 6st October 2014 to 06 April 2015.

WORK EXPERIENCE

Worked as a Lecturer at Sri Sarada Degree and PG College Ameerpet Hyderabad from January 2014 to September 2014

WORK EXPERIENCE

Worked as Assistant Microbiologist at L.V.Prasad Eye Institute,Hyderabad from September2011 to September 2013

Profile Summary

Diagnostics and Research in medical microbiology.

Maintenance of Internal and External quality control in microbiology.

Maintenance of NABH and NABL records.

Monthly surveillance of operation theaters and laboratories.

Maintenance of ATCC standard strains.

Analysis of the Monthly CQI parameters.

Working as an Investigator in a study entitled “ACSIKS” (Asia Cornea Society Infectious Keratitis Study)

Worked on a project entitled “Antifungal susceptibility testing of ocular fungal isolates (likeFusarium species, Aspergillus species, etc.) and determination of MIC by E-Test”.

Efficacy of U.V and riboflavin on different organisms(phase -I)

Efficacy of U.V and riboflavin on different multi drug resistant organisms(Phase-II)

Collaboration project of SERI(Singapore eye research institute)[Determing the susceptibility of a peptide using multi drug resistant organisms like pseudomonas & MRSA(Methillicin resistant staphylococcus aureus.

Data analysis of each month as well year too.

Preparation of different eye drops[like Povidine iodine solution, avastin, PHMB,Chlorohexidine,etc]

Demonstrated ability to prepare technical reports and research papers.

Hands on experience in diagnostic problems involved in determining the cause and control of disease

PUBLICATIONS

“Ocular infections caused by Candida species: Type of species, in vitro susceptibility and treatment outcome” published in Indian journal of medical microbiology; volume: 33 Number: 4, October 2015

CONFERENCES ATTENDED

Participated in “EVIDENCE-2012”an international CME on Evidence-Based Ophthalmology, organized by the LV Prasad Eye Institute, Hyderabad, from May 31 to June 3, 2012.

Participated in “IERG-ARVO-IC”(Indian Eye Research Group-ARVO-India chapter) 20th annual meeting, jointly organized by LV Prasad Eye Institute, Centre For Cellular And Molecular Biology and ARVO Chapter Affiliate, Hyderabad, from July 28 to July 29, 2012

EXPERIENCE IN MICROBIOLOGY

Bacteriology:

Identification of bacteria from ocular and other specimens.

Processing of samples in the laboratory

Plating techniques

Wet mount preparations

Staining techniques and identification of stained smears and its reporting.

Trained with microscopic examination of smears

Isolation and identification of bacteria

Sensitivity testing and MIC determination

Quality control for culture media, antibiotic discs and stains.

Sterilization techniques.

Media preparation.

VITEK system for identification of bacteria

Parasitology:

Identification of parasites in ocular specimens/samples

Wet mount preparations and staining techniques

Mycology:

Identification of fungal isolates in clinical specimens

Different culture techniques used for fungal identification

Wet mount preparation of samples and other staining techniques and interpretations.

Molecular techniques:

Isolation of DNA from clinical samples

Isolation from culture.

Electrophoresis

PCR(polymerase chain reaction)

Knowledge about sequencing PCR, gradient PCR.

Virology:

Cultivation of viruses through embyonated eggs

Immunology:

Quantiferon TB gold – ELISA method

Antinuclear antibodies (ANA) Fluorescent technique

Determination of ABO grouping by slide agglutination method

Immunoflourescence assay test (IFA).

Serology:

HIV, HCV & Hbs - antigen & antibody detection by spot & Elisa method.

VDRL by Rapid plasma reagin method (flocculation analysis)

ELISA

PROJECT DETAILS

Title: Antifungal susceptibility testing of ocular Candida isolates and determination of MIC by E-test

Environment: L.V.Prasad Eye Institute, Hyderabad

Duration: 2010 to 2013

Samples: 35 samples

Unit: Jhaveri Microbiology Centre, working under Dr. Savitri Sharma, Dr. Swapna Reddy M

My role involved collection and microbiological testing of ocular isolates and determination of MIC by E-test. The results were statistically analyzed and the results are presented for a publication yet to be sent.

TECHNICAL SKILLS

Packages : MS-Office, PGDCA.

Operating System : Windows 98/XP/NT/2000/2003,Vista.

EDUCATIONAL QUALIFICATIONS

M.Sc. (Microbiology), with 72%, Osmania University, A.P, India.

B.Sc. in Bt.Mb.C (Biotechnology, Microbiology, Chemistry), with 70%, Osmania University, A.P, India.

Intermediate in Bi.P.C (Biology, Physics, Chemistry), with 74%, Sravanthi junior College, Zaheerabad, A.P.

S.S.C, with 82%, Gandhi Memorial High School, Zaheerabad, A.P.

PERSONAL STRENGTHS

Self-motivated and disciplined.

Ability to work with multi-cultural teams.

Ability to take up new challenges

Profound ability to work individually and in a team

Able to work additional hours if required

Outstanding physical and mental dexterity

Excellent grasp of microbiological terminology

Self directed and organized

Willingness to learn and follow directions

Excellent communication skills

HOBBIES AND INTRESTS

Playing shuttle

Playing chess

Painting

PERSONAL INFORMATION:

Father’s Name : N.NagiReddy

Date of Birth : 29/Nov/1988

Nationality : Indian

Marital Status : Married

Languages Known : English, Telugu, Hindi and Kannada

N.VIDYARANI

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