Data Medical

RITU PATEL

** **. ***** ******, ****** City, NJ *7306

Phone: 201-***-**** E-mail: ******@*******.***

Seeking a position as a scientist that allows personal growth while making meaningful contribution to organization’s success.

TECHNICAL SKILLS

Biochemical and Molecular Techniques – Qualitative analysis of proteins and carbohydrates; subculturing of cells; SDS-PAGE and agarose gel electrophoresis; Use of calcium phosphate and lipofaction methods (escort methods) to transfect DNA inside the cell; Western Blot; DNA and RNA isolation; Cloning and Subcloning into the mammalian cell expression vector; plasmid isolation;

Immunological Techniques – Use of Hematoxylin and H&E staining method to see ICER protein expression in prostate cancer tissues; Blood grouping, RH types, blood smears, total and differential count of WBC’S and RBC’S, Widal test, blood serum preparation and analysis;

Collecting and Analyzing water, air, soil, and waste water samples;

Operation of Spectrophotometer and RPHPLC;

EDUCATION

Montclair State University, Montclair, New Jersey May 2011

Master of Science in Molecular Biology GPA 3.8

Sardar Patel University, India April 2004

Master of Science in Environmental Science GPA 4.0

Sardar Patel University, India April 2000

Bachelor of Science in Biotechnology GPA 2.9

Gujarat School of Medical Technology, India June 2002

Diploma in Medical Laboratory Technology GPA 4.0

EXPERIENCE AND TECHNICAL TRAINING

Scientist 1

PDS Biotechnology, North Brunswick, NJ May 2015 - Present

Working with cancer vaccine development, formulation, and characterization group

Prepare formulated peptides or protein drug products by using Nano - particle / liposomes based techniques

Characterize drug products by using SDS -PAGE, RP HPLC, and particle size analysis and spectroscopy techniques.

Knowledge of Transmission Electron Microscope (TEM).

Assay Qualification, preparing reports and discussing data with supervisor

Maintain experimental details in laboratory notebooks

Manage laboratory supplies and reagent

Lab Technologist

Labcorp, Raritan, NJ April 2014 –February 2015

Working in Esoterix Immunology department the basic duties are there- Use of FLOW CYTOMETERY technique to observe the Lymphocytic B and T cells and certain Activation markers this includes

Prepare blood samples using BD Sample Prep Assistant III (BD SPA III) instrument;

Analyze data using DIVA and CANTO software of BD CANTO2 instruments; data is analyzed based on gating and calculation criteria for different population of Lymphocytes i.e. CD3, CD4, CD8, CD19, CD16+56 as well as certain activation marker for T-cells such as HLADR,CD3/25, CD8/38, CD8/57, etc;

Run reagent parallel for the new lots of reagent and enter the value in excel for statistical analysis between the old and new lots of the reagents

Use of Western Blot Technique for screening patient’s sample for the presence of IGg and IGm anti-bodies to confirm presence of Lyme disease

Scientist

Pfizer, Pearl River, NY April 2012 –October 2012

Working with C Diff. vaccine research group performing cells cytotoxicity and viability assay for toxins and toxiods of C Diff. as well as also performing the cell neutralization assay with the hamster sera

Maintaining the HT29 and IMR-90 cell line by subculturing, plating cells for assays, treating cells with diluted toxins or toxids samples;

Evaluating results for cell cytotoxicity, viability and neutralization by CPE as well as ATP method by using Wallac Envision;

Preparing graphs from the results by using Graph-Pad and discussing data with supervisor;

Maintaining experimental details in laboratory notebooks;

Helping with Toxoid preparation by doing downstream work such as bacterial transformation, plasmid prep ( Mini-Maxi), restriction digestion of plasmid DNA, gel run of digested DNA, and sequencing DNA

Lab Specialist October 2010 – March 2011

Hoffmann-La Roche, Nutley, NJ

Screening compounds to determine the inhibitory effects of anti HCV compounds on genotype 1a (GT1a) and genotype 1b (GT1b) cell lines; assistance with preparing in vitro HCV transcript

Plating and treating the cells with different concentration of anti HCV compounds;

Analyzing data using luminometer;

Bacterial transformation, plasmid DNA isolation, using midi or maxi kits, restriction enzyme digestion, IVT for the preparation of RNA

Research Associates September 2009 – May 2010

Schering Plough / Merck, Kenilworth, NJ

Screening compounds for CYP450 enzyme induction using rat liver slices / CYP450 induction study in Cryo-preserved Human Hepatocytes

Plating cryo-preserved primary human Hepatocytes and treating cells with proprietary compounds

Isolating mRNA using robotic liquid handling instrument (Siclone ALH3000)

Using RT-PCR to prepare cDNA

Quantifying CYP450 gene expression using Taqman (ABI 7900)

Analyzing samples, preparing reports and discussing data with supervisor

Maintaining experimental details in laboratory notebooks

Managing laboratory supplies and reagents

Student Research Spring 2009

Activation of Protein Kinase into HEK 293T cell

Cell culturing;

Transfection of Plasmid in HEK 293T cell;

Protein extraction;

Plasmid preparation (plasmid midiprep system);

Western Blot, dot blot;

SDS-PAGE;

Protein Assay – Bradford and BCA Assay

Expressing ICER in chicken for hyper ovulation Fall 2008

Isolation of DNA and RNA;

Use of PCR and RT-PCR techniques;

Agarose Gel Electrophoresis;

Transfection of cloned DNA;

Cloning and subcloning in the mammalian cell expression vector;

Plasmid isolation

REFERENCES AVAILABLE UPON REQUEST

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