NEELAM MISHRA

***** *. ******** ****, ***#***, Lake Bluff, IL-60044

+1-806-***-****, acwm59@r.postjobfree.com

CAREER OBJECTIVE

Team oriented, creative and technically experienced molecular biology Ph.D. seeking full time employment as a research associate/ scientist

EDUCATION

Ph.D., Biological Sciences, 2010- 2016, GPA 3.976/4.00

Texas Tech University, Lubbock, USA

Dissertation topic: Over-expression of rice SUMO E3 Ligase gene OsSIZ1 improves both drought and heat tolerance in transgenic cotton

M.Tech., Applied Botany, 2007- 2009, GPA 3.9/4.00

Indian Institute of Technology, Kharagpur, India

M.Sc., Botany, 2003- 2005, GPA 3.987/4.00

D.D.U.G.U Gorakhpur University, India

RESEARCH EXPERIENCE

Ph.D. Research (July 2010- August 2016)

Genetic engineering of plants such as cotton and Arabidopsis for increased abiotic stress

tolerance

Role of one of regulatory subunits of protein phosphatase 2A in Arabidopsis thaliana

TECHNICAL SKILLS AND EXPERTISE

Molecular Biology skills: DNA and RNA extraction, Plasmid isolation, Gene cloning, Northern, Southern blotting, cDNA synthesis, PCR, quantitative PCR (ABI 7500 and light cycler 480), E. coli, Agrobacterium and Yeast transformation, Site-directed mutagenesis

Biophysical methods: Separation and identification of metabolite using High Performance liquid chromatography, Automated low pressure liquid chromatography system equipped with gel filtration and ion-exchange columns, Spectrophotometry, Nanodrop quantitation, colorimetric bioassays using microplate reader

Protein biochemistry: Protein extraction, SDS PAGE, Western blotting, Protein expression and purification, protein- protein interaction studies using yeast-two hybrid system

Immunological techniques: ELISA, Immuno-precipitation

Cell culture techniques: plant tissue culture, microbial cell culture Agrobacterium mediated plant transformation

Bioinformatics skills: BLAST, ClustalW, DNAMAN, Primer designing, NEB cutter, Vector NTI etc.

Computer skills: MS word, MS Excel, MS powerpoint, ANOVA, Adobe Photoshop, Sigma Plot

AWARDS AND FELLOWSHIPS

•Summer Dissertation award from Graduate School, Texas Tech University, 2016

•Second Prize in Oral Presentation at Beltwide Cotton Conferences, New Orleans, Louisiana, 2016

•Texas Tech University Doctoral Dissertation Completion Fellowship, Graduate School, Texas Tech University, 2015

•Preston and Ima Smith Scholarship, Graduate School, Texas Tech University, 2014

•First Prize in 13th Annual Graduate School Research Poster Competition, Texas

Tech University, 2014

•Third Prize in 13th Annual Graduate School Research Poster Competition, Texas

Tech University, 2013

•University Gold Medals in M.Sc. Botany from D.D.U.G.U., Gorakhpur, India, 2005

•Study Abroad Competitive Scholarship, Office of International Affairs, Texas Tech

University, 2013, 2014, 2015 and 2016

•Water Conservation Research Scholarship, Graduate School, Texas Tech University, 2012

•TEACH Fellowship 2012, Teaching, Learning and Professional Development Center

(TLPDC), Texas Tech University

PUBLICATIONS

•Mishra N, Sun L, Zhu X, Srivastava A.P, Yang X, Luo H, Burke J, Payton P, Zhang H. Expression of the rice SUMO E3 ligase gene SIZ1 enhances both drought and heat tolerance in transgenic cotton. Plant Biotechnology, soon to be submitted

•Mishra N, Srivastava A.P, Shen G, Zhang H (2016). Improvement of multiple abiotic stress tolerance in Arabidopsis by over-expression of the rice SUMO E3 ligase gene SIZ1. Plant Cell and Environment, in preparation.

•Kuppu S, Mishra N, Hu R, Sun L, Zhu X, Shen G, Blumwald E., Payton P, Zhang H. (2013). Water Deficit Inducible expression of a cytokinin biosynthetic gene improves drought tolerance in transgenic cotton. PLoS One. May 10; 8(5):e64190. doi 10.1371/journal.pone.0064190.

•Hua Q, Qiang G, Kuppu S, Sun L, Zhu X, Mishra N, Hu R, Shen G, Zhang J, Zhang Y, Zhu L, Zhang X, Burrow M, Payton P, Zhang H. (2013). Expression of the Arabidopsis vacuolar H+-Pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance Plant Biotechnology Reports 7: 345-355.

•Pehlivan N, Sun L, Jarrett P, Yang X, Mishra N, Chen L, Shen G, Kadioglu A, Zhang H. (2016). Co-overexpressing a plasma membrane sodium/proton antiporter and a vacuolar membrane sodium/proton antiporter significantly improves salt tolerance in transgenic Arabidopsis plants. Plant Cell and Physiology, 57 (5): 1069-84.

CONFERENCE PRESENTATIONS (SELECTED)

•Oral presentation entitled as “Heterologous expression of the rice gene OsSIZ1 improves both drought and heat tolerance in transgenic cotton” in Belt Wide Cotton Conference 2016, New Orleans, Louisiana

•Presented a poster entitled as “Heterologous expression of the rice gene OsSIZ1 improves both drought and heat tolerance in transgenic cotton” in Belt Wide Cotton Conference 2015, San Antonio, Texas

•Presented a poster entitled as “Heterologous expression of the rice gene OsSIZ1 improves both drought and heat tolerance in transgenic cotton” in the Workshop Ogallala Aquifer Program 2014, Lubbock, Texas

•Presented a poster “Water Deficit Inducible Expression of a Cytokinin Biosynthetic Gene IPT Improves Drought Tolerance in Cotton” at the Annual Meeting of American Society of Plant Biologists (ASPB) 2012, Austin, Texas

REFERENCES

Available upon request

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