Anderson Ayuk Agbor

**** ****** *****, *****, ** 75074

Tel. +1-469-***-**** Email: acwkdu@r.postjobfree.com

https://www.linkedin.com/in/ayukanderson1

CAREER SUMMARY:

Highly motivated and dedicated professional Biochemist with extensive research experience in two therapeutic areas including neuromuscular diseases, and cancer (DNA damage and repair). Experienced in cell-based assay development and optimization, antibodies production and purification, isolation of cells and generation of primary cell lines from human and mouse tissues etc. Hands-on experience in molecular biology applications including RNA/DNA extraction, purification and sequence analysis, site-directed mutagenesis construction, plasmid vectors construction, DNA amplification (PCR, RT-PCR), siRNA, molecular cloning. Experience with purification and analysis of recombinant proteins. Have consistently run and troubleshoot assays in different platforms including protein binding and analysis, ligand-receptor binding kinetics, and qPCR to study the pattern of gene expression. Also knowledgeable in FDA regulations and ICH/GCP guidelines for the conduct of toxicology and Clinical studies as well as testing of non-clinical and clinical samples.

RELEVANT TECHNICAL SKILLS (Bench work experience)

Molecular Biology: Expression vector design. Molecular cloning of genes. Expression and purification of recombinant proteins in mammalian and bacterial cells using FPLC affinity and size chromatographic purification system. RNA/DNA extraction from cells and tissues. DNA amplification by PCR, gel electrophoresis and sequencing analysis.

Construction and screening of cDNA library. Site directed mutagenesis. Chromatin immunoprecipitation (ChIP) assay, electrophoretic mobility shift assay (EMSA). Labeling of bimolecular probes with radioactive species and antibodies. In-situ hybridization (ISH) technique using radioactively labeled probe as well as antibodies. siRNA knock down, gene expression analysis using RT-PCR, Northern blot and Southern blot techniques.

Cell Biology: Mammalian cell culture: Growth, development and optimization of HCT116, Caco-2, Hek-293, CHO, COS-7 cells for cell-based assay. Immunization of mice and rabbits, collection of blood and spleen for monoclonal (Hybridoma technique) and polyclonal antibody production and purification. Affinity purification of immunoglobulins (antibodies) using protein A resins-packed column, as well as protein G beads. Experienced in proteins solubilization buffers including triton X-100, Tween-20 (non ionic detergents), SDS (ionic detergent) and CHAPS (zwitterionic detergent). Experienced with the creation of animal and cell models of human cancer. Biosafety Level 2 (BSL-2) lab experience - working with human samples including whole blood, and human skeletal muscle. Peripheral blood mononuclear cells (PBMC) isolation from whole blood and immortalization of B-lymphocytes. Cell sorting experience using FACS Aria.

Biochemistry: Immunochemical and biochemical assays for the analysis of proteins and protein binding: SDS-PAGE western blot analysis, ELISA using immunofluorescence (direct, indirect, sandwich) ADA, electrochemiluminiscence. Preparation of microsome from liver hepatocytes to analyze metabolites, and the hippocampus of rat brain to evaluate receptor-ligand binding equilibrium. Extensive experience with ISH, Immunohistochemistry (IHC), immunocytochemistry. Immunoprecipitation (IP) and Co-IP procedures and optimization of IP protocols for protein-protein interactions studies.

Lab Instruments: Familiarity with: scale balances, protein gel electrophoresis apparatus, Nucleic acids electrophoresis, Nano drop, chemiluminiscence gel imager, fluorescence and confocal microscopy, spectrophotometers, flow cytometer for cell analysis based on fluorescence, FPLC for purification of proteins. Fluorimeter, RT-PCR, thermal cycler for PCR, Beckman Coulter liquid scintillation radioactive counter. Table top centrifuges, high speed, analytical and ultra centrifuges. Familiarity with FACS Aria, Surface Plasmon (SPR) Resonance for protein interactions and affinity studies.

STRENGTHS AND CAPABILITIES:

Excellent communication and organizational skills and a professional demeanor.

Quality oriented individual, with ability to build productive working relationships with management, co-workers and internal/external clients.

A diligent and extremely hard worker, detail oriented with excellent time management skills to meet daily metrics or team objectives.

Excellent presentation skills as exemplified in my scientific conferences attended.

Skilled in MS Office (Excel, Word, Outlook, PowerPoint), MS-SQL DBA & T-SQL, Photoshop Adobe, and GraphPad Prism, Image J software.

Experience with web-based search tools including PubMed, NCBI etc and Bioinformatics tools for protein analysis including, sequence similarities comparison through multiple sequence alignment (ClustalW, LALIGN, and ExPASy etc), protein-protein interaction prediction and, proteins structure and function prediction, as well as mutation sequence identification.

EDUCATION Ph.D. (Cum Laude) in Biochemistry, University of Basel, Switzerland, 2000-2003

Thesis: JP-45, a novel protein of the skeletal muscle sarcoplasmic reticulum junctional face membrane: molecular cloning, tissue distribution, developmental expression, and functional interaction with other sarcoplasmic reticulum proteins involved in skeletal muscle excitation contraction coupling.

Research Advisor: Professor Susan Treves.

B.Sc/M.Sc in Biological Sciences (Pathophysiology) University of Ferrara, Italy, 1993-1998

Thesis: Biochemical and Pharmacological characterization of the interaction between cyclic adenosine ligands and adenosine receptor A1

Research Advisor: Professor Pier Andrea Borea – Department of Pharmacology.

CERTIFICATES Research Project Management Certificate: Massey University, Palmerston North, New Zealand (2006)

Clinical Research Development Certificate: The Clinical Research Institute, Houston, TX (2013)

RESEARCH ACTIVITY

Extensive research skills on human colorectal cancer, as well as in neuromuscular diseases affecting human skeletal muscle.

Neuromuscular Disease:

Specific disease areas of my previous work:

1) a) Malignant hyperthermia (MH) - a genetic disorder of the skeletal muscle whose phenotype is characterized by severe and tetanic muscle contraction accompanied by a rapid rise in body temperature and increased heart beat, triggered by volatile inhalational anesthesia and muscle relaxants like succinylcholine in patients harboring the causative mutation on the ryanodyne receptor. Dantrolene sodium is the common medication used preoperatively on suspected MH patients and for managed care postoperative.

b) Central Core Disease (CCD) and Multiminicore Disease.

Oncology:

Specific disease areas of previous work:

1) Colon cancer – Mutations and/or genetic alterations in DNA polymerase epsilon associated with colorectal cancer.

2) Prostate cancer and metabolic Syndrome - the role of metabolic syndrome (over nutrition, hyperglycemia, hyperlipidemia, insulin resistance, and obesity) in chronic inflammation of the prostate and tumor formation. Addressing the pro-inflammatory pathways activated by adipocytes, which initiate and promote the growth and migration of androgen-dependent prostate cancer cells. Studies on the population distribution of inflammatory markers in tumor tissue by ethnic origin.

3) Non-Small Cell Lung Cancer (NSCLC) - histone deacetylase 6 (HDAC6) regulation in non-small cell lung cancer by specific inhibitors that target the Tgf-beta and notch signaling pathways in NSCLC. Phamacological class 2 hdac inhibitors including Tubacin, ACY1215, and trichostatin A (TSA) are of particular interest, and human lung adenocarcinoma cell lines A549 are preclincally used for lab.

WORK EXPERIENCE:

Associate Professor of Biology: Collin County Community College, Frisco, TX 01/21/2020 - Present

I teach Anatomy and Physiology I and II courses to students of the above mentioned college.

Teaching and Tutoring: Private evening and Weekend classes. 10/2016 – 12/2019

I organize and teach biology and chemistry courses to both college and high school students in the evenings and weekends, as well as revision classes in physiology to few LPN student nurses.

Reliant Energy, Dallas, TX

Commercial Energy consultant (business experience) 11/02/2014 -10/30/2016

Delivered professional presentations and proposals to exceed client needs and expectations and ensured that client issues were resolved in a timely manner.

Worked with management to continually refine marketing strategies to optimize results

Remained current on industry news and market conditions and communicated this info to clients

Effectively negotiated and closed deals and consistently followed-up with clients to ensure customer and consumer satisfaction.

Tulane University School of Medicine, New Orleans, LA 09/2010 – 07/2013

Postdoctoral Research Fellow - Tulane Cancer Center

Conducted research project that was focused on investigating the impact of a defective DNA polymerase on DNA replication, damage and repair, genomic instability and the onset of colon cancer. I used HCT116 colon cancer cell lines MLH-1 +ve and –ve, as well as p53 null cells as my model system.

Made vector constructs, introduced site directed mutations, incorporated these mutants into mammalian cells, and examined fluorescence with fluorescence microscopes and FACS. Also performed in-vivo and in-vitro experiments to assess (1) the physiological relevance of theses mutants, (2) the cytoprotective effect of the cells exposed to H2O2 and other toxic compounds.

Assembled data and wrote manuscripts to be published in peer-reviewed scientific journals.

Supervised and advised graduate students and lab technicians and helped to interpret data for graduate students and colleagues, as well as identifying and troubleshooting their complex technical problems.

Participated in training of team members, research colleagues, and students to ensure proficiency of all assays and improved instrument techniques. Also maintained and calibrated laboratory instruments according to SOPs.

Participated in new technology and instrument evaluations and implementation, and received extensive training on institution’s GLP to ensure proficiency and compliance with laboratory operations.

Brigham & Women's Hospital, Harvard Medical School, Boston, MA 07/2007 - 06/2009

Postdoctoral Research Scientist - Dept of Anesthesia

Conducted Basic research on skeletal muscle physiology.

Constructed expression vectors, performed molecular cloning and expressed and purified recombinant proteins.

Prepared lab reagents for research purpose.

Massey University, Palmerston North, New Zealand 08/2005 - 06/2007

Postdoctoral Research Scientist - Institute of Molecular Biosciences

Conducted basic and clinical research on malignant hyperthermia (MH) syndrome, a genetically inherited myopathy of the skeletal muscle affecting diverse family members in New Zealand.

Isolated B-lymphocytes from human blood of MH patients and fused with myeloma cells to form hybridoma cells which were subsequently purified, cultured, and used to study calcium homeostasis, utilizing both radiometric and biochemical assays including live cell imaging of calcium release with confocal microscopy.

Collected and analyzed biological data, and studied the correlation between MH susceptible individuals and MH causative mutations.

Assisted PI in writing manuscript for publication of research findings in peer reviewed journal.

Participated in lab weekly and department monthly meetings with regular presentation of research data.

University Hospital of Basel, Basel, Switzerland 04/2003 - 07/2005

Postdoctoral Research Scientist - Department of Anesthesia and Research

Conducted basic research on skeletal muscle physiology and taught Biology courses to undergraduate students of the Biological Science faculty. Expressed and purified recombinant proteins for use in IP and CoIP experiments

Investigated the impact of intrinsic target properties on skeletal muscle function utilizing siRNA (knockdown).

Managed some lab functions like supervision of lab technicians, students, ordering of lab supplies, preparation of reagents for lab use and maintenance of lab notebook.

Produced and characterized recombinant proteins, polyclonal and monoclonal antibodies and analyzed these proteins by western blot and ELISA.

Cared for mice and rabbits for my research and assisted PI in drafting research project plan.

Conducted research work in collaboration with our partners in Grenoble, France which required my frequent travel to France.

Applied knowledge of good laboratory practices on a daily basis.

AWARDS:

2011: Recipient of Tulane Cancer Center’s 2011 Matching Funds for Cancer Research.

2012: Recipient of a Postdoctoral travel award to attend the ASBMB 2012 Annual Meeting,

held in conjunction with FASEB, in San Diego, CA, April 21-25, 2012.

2013: Recipient of the Tulane University, Dept. of Biochemistry Postdoctoral Research Fellow

Best Research Paper of the year 2013 Award (Certificate of Achievement and a Monetary Award).

CONFERENCES ATTENDED:

2000: International Conference in Anesthesiology: In Basel, Switzerland.

2002: Biophysical Society Meeting: In San Antonio, USA.

2003: Gordon Research Conference: Poster presentation; NH, USA.

2012: Federation of American Societies for Experimental Biology (FASEB) meeting: Poster presentation; San Diego, CA

2010: Seminar Speaker - JP-45 Protein: Dept. of Physiology & Biophysics seminar at Albert Einstein College of Medicines, Yeshiva University, New York.

LANGUAGES: Fluent in English, French and Italian (Oral & written)

AFFILIATION: Registered member of: American Association for the Advancement of Science (AAAS).

National Association of Pharmaceutical Representatives (NAPSR)

IMMIGRATION WORK STATUS: US Citizen

PEER REVIEWED PUBLICATIONS:

■ Francesco Zorzato, Ayuk A. Anderson, Kai Ohlendieck, Gabriel Froemming, Remo Guerrini, and Susan Treves. Identification of a novel 45 KDa protein from rabbit sarcoplasmic reticulum junctional face membrane. Biochem. J, 2000; 351: 537-543.

■ Ayuk A. Anderson., Treves, S., Zorzato, F., Biral, D., Betto, R., Sandonà, D. and Ronjat, M. The Novel Skeletal Muscle Sarcoplasmic Reticulum JP-45 Protein: molecular cloning, tissue distribution, developmental expression and functional interaction with α1.1 of the voltage-gated calcium channel. J. Biol. Chem. 2003; 278: 399**-*****

■ Ayuk A. Anderson., Treves, S., Zorzato, F. Characterization of JP-45 and interaction with proteins involved in skeletal muscle EC-coupling. Biophysical Journal 2003; vol. 84, No 2 Abstract No 2818

■ Susan Treves, Ayuk A. Anderson, Sylvie Ducreux, Alexandra Divet, Christophe Bleunven, Cristiano Grasso, Silvia Paesante, Francesco Zorzato. Ryanodine receptor 1 mutations, dysregulation of calcium homeostasis and neuromuscular disorders. Neuromuscular Disorders 2005; 15: 577-587

■ Divet, S. Paesante, C. Bleunven, A. Anderson, S. Treves, F. Zorzato. Novel sarco/endoplasmic reticulum proteins and calcium homeostasis in striated muscles. J. Muscle Res Cell Motil 2005; 26: 7-12

■ Ayuk A. Anderson, X. Altafaj, Zheng Z, Wang Z, M. Ronjat, O. Delbono, S. Treves, F. Zorzato. The junctional sarcoplasmic reticulum JP-45 protein affects the functional expression of the voltage dependent calcium channels CaV1.1. J. of Cell Science, 2006; 119: 2145-2155.

■ E. Guoadon, R. P. Schuhmeier, D.Ursu, Ayuk A. Anderson, S. Treves, F. Francesco, F. Lehmann-Horn, W. Melzer. A possible role of the junctional face protein JP-45 in modulating calcium release in skeletal muscle. J. Physiol. 2006; 57: 269-280.

■ Ayuk A. Anderson, Rosemary L. Brown, Brenda Polster, Neil Pollock, Kathryn M. Stowell. Identification and biochemical characterization of a novel ryanodine receptor mutation associated with malignant hyperthermia. Anesthesiology 2008; 108: 208-215

■ Ayuk A. Anderson. Reactive oxygene species: their role in severe clinical conditions and regulation of redox-sensitive cell signaling transduction events. Annals of Biological Research, 2011; 2: 599-609

■ Anderson Ayuk Agbor, Yasemin A. Goksenin, Kimberly G. LeCompte and Zachary F. Pursell. Human Pol e-dependent replication errors and the influence of Mismatch Repair on their Correction. DNA Repair, 2013; 12: 954-963

POSTER PRESENTATIONS:

■ Ayuk A. Anderson., Treves, S., Zorzato, F. Characterization of JP-45 and interaction with proteins involved in skeletal muscle EC-coupling. June 2003: Gordon Research Conference, NH,

■ Agbor A. Anderson, Dagmara A. Korona, Kimberly G. LeCompte and Zachary F. Pursell

Expression & Characterization of the exo- M630G mutant allele of human Pol ε catalytic subunit in human colon cancer cell lines, HCT116. April 2011: Louisiana Cancer Research Consortium (LCRC), New Orleans, LA

■ Ayuk A. Anderson and Pursell F. Zachary. An active site mutant allele of human Pol e is a mutator in vitro and in vivo. April 2012: Experimental Biology Meeting, San Diego, CA

■ Ayuk A. Anderson, 2010: Seminar Speaker - JP-45 Protein: Dept. of Physiology & Biophysics seminar at Albert Einstein College of Medicines, Yeshiva University, New York.

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