Education:
MCPHS University, Boston MA
Master of Science in Pharmaceutical Sciences, Received, May 2016
Graduate GPA: 3.95/4.00
Award: Graduate Studies Scholarship, September 2015
Bachelor of Science in Pharmaceutical Sciences, Received, May 2015
Employment:
Dicerna Pharmaceuticals, Preclinical Development, Cambridge MA
June-October 2016
Intern (full-time)
Direct Supervisor: Girish Chopda
Develop innovative RNAi therapies for the treatment of rare diseases, such as chronic liver diseases, cardiovascular disease, and viral liver infectious diseases
Collaborate with company teams to investigate and improve pharmacokinetics/pharmacodynamics parameters of current siRNA models
Design and execute in vivo/in vitro screenings involving siRNA based formulations
oEvaluating pk/pd of siRNA conjugates with or without chemically optimized modifications
Determine and quantify guide-strand exposure and target-gene knockdown
oValidating activity/potency of siRNA conjugates
Determine the lowest effect concentration with appropriate cell-lines
Forward/reverse transfection, cytotoxicity assays
Validate drug pk/pd by closely follow standard operation procedures to perform downstream analysis
oRNA isolation, cDNA synthesis, RT-PCR, Stem-Loop RT-PCR, ELISA, immunohistochemistry, microscopic imagery
Dose animals via various routes of administration, necropsy, tissue/blood sample collections
Troubleshoot/validate/optimize current in vivo and in vitro models
Graph/interpret/analyze findings on available software programs
Weekly meetings to present/share/discuss results and progress
MCPHS University, School of Pharmacy, Boston MA
December 2015-June 2016
Graduate Research Intern (full-time)
Direct Supervisor: Amanda Kentner
Investigating adolescent pain responses and underlying gene expression profiles across important brain areas involved in pain processing, in rats that were exposed to neonatal immune-challenges (See journal article below under publication)
Developing and executing behavior studies with Sprague-Dawley rats by implementing well-recognized models
oMaintenance of long-term behavior experiments
Behavior analysis
oOpen-field filming
Light-dark test
oMechanical allodynia test
Von Frey hair
Animal surgery/perfusion/dissections
otissues/blood/fetus/fecal collections for downstream analysis
RNA/DNA extractions, cDNA synthesis, RT-PCR and ELISA for the whole project
MCPHS University, School of Pharmacy, Boston MA
August 2015-May 2016
Graduate Teaching Assistant
Direct Supervisor: Mark Bohlke
Teaching undergraduate students to properly design and execute scientific experiments by following standard operation procedures
oFormulating drug tablets, capsules and suspensions
oPreparing solutions/buffer for each experiment
oSample analysis using spectrophotometer, HPLC, bioreactors or other appropriate instruments
oFormulating standard curve for calibrations
oResult analysis and interpretation
Laboratory maintenance, cleaning, setting up, ensuring all materials are in stock
Grading homework, quizzes, reports
Proctoring examinations
Publications:
Yan, S., Kentner, A.C. Mechanical allodynia corresponds to Oprm1 downregulation within the descending pain network of male and female rats exposed to neonatal immune challenge. Brain Behav. Immun. (2016) http://dx.doi.org/10.1016/j.bbi.2016.10.007
Yan, S. and Kentner, A.C. (2016). Neonatal inflammation induces mechanical allodynia and sex-specific gene expression in brain areas involved in the active modulation of nociception and inflammatory pain
To be presented at the Society for Neuroscience meeting in San Diego, California in November 2016
Skills:
Molecular Biology Techniques: cell cultures, cytotoxicity assays, apoptosis assays, transfection, RNA/DNA extraction, cDNA synthesis, RT-PCR, Stem-Loop RT-PCR, ELISA, BCA assay, immunohistochemistry, staining, microscopic images
Animal Handling: develop studies involving animal modeling, behavior experiments, dosing, animal-takedown and sample collections
Literature Research: analyzing the applicability of existing methods to current research, using innovative techniques to replicate and improve these methods without having all the necessary resources available
Laboratory Skills: meticulous note-keeping in both laboratory notebooks and online files, precise mechanical pipetting skills, maintaining an organized and clean work space
Microsoft Excel: advanced in using excel functions, formulating calculation templates, data organization/analysis/interpretation
Hobbies:
Dragon boat, rowing, violin
References:
Girish Chopda, PhD, Principal Scientist at Dicerna Pharmaceuticals, Cambridge
Girish was Siyang’s supervisor at Dicerna Pharmaceuticals
617-***-**** acw96x@r.postjobfree.com
Amanda Kentner, PhD, Assistant Professor of Psychology at MCPHS University, Boston
Dr. Kentner was Siyang’s research internship supervisor at MCPHS University
617-***-**** acw96x@r.postjobfree.com
Gerard D’Souza, PhD, Associate Professor of Pharmaceutics at MCPHS University, Boston
Dr. D’Souza was Siyang’s academic advisor and professor for three academic courses at MCPHS University
617-***-**** acw96x@r.postjobfree.com