Maria Isabel Stets

Curriculum Vitae

Personal data

Name Maria Isabel Stets

Date of Birth 05/06/1981 - Ponta Grossa/PR - Brazil

Home address Street Claudio Pereira da Cruz, 1268, apt 14

Iná – São José dos Pinhais

Zip code: 83065-020, PR - Brazil

Phone: +55-41-960*-****

Electronic address acw7qr@r.postjobfree.com

Academic background

2009 - 2013 Doctorate degree in Science (Bioquemistry).

Universidade Federal do Paraná, UFPR, Curitiba, Brazil with a sanduich period at HELMHOLTZ ZENTRUM MUNICH (Supervisor: Anton Hartmann)

Title: Monitoring Azospirillum brasilense and study of bacterial diversity associated with wheat roots (Triticum aestivum), year: 2013

Supervisor: Leonardo Magalhães Cruz

Scholarship from: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

2006 - 2008 Master degree in Food Science and Technology.

Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, Brazil

Title: The influence of support materials and characterization of microbial diversity in anaerobic reactors for slaughterhouse effluent treatment, year: 2008

Supervisor: Ana Cláudia Barana

2001 - 2005 Garduation in Biological Sciences.

Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, Brazil

1997 - 2000 Technical Hight School. Food Technology.

Universidade Tecnológica Federal do Paraná, UTFPR, Curitiba, Brazil

Pos-doc

2015-2016 Parana Molecular Biology Institut, IBMP, Curitiba, Brazil

Development of DNA aptamers against the viral p24 antigen (HIV) and Zika virus for diagnosis tests.

Scholarship from: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

2013 - 2015 Laboratoire des Symbioses Tropicales et Méditerranéennes, LSTM, França

Obtaining Herbaspirillum endophytic strains capable of nodulate legumes.

Scholarship from: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

2013 - 2013 Universidade Federal do Paraná, UFPR, Curitiba, Brazil

Structural and functional analysis of Azospirillum brasilense GlnB protein.

Scholarship from: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

Research Projects Participation

2015 – Actually Development of DNA aptamers for use in diagnose kits. Paraná Molecular Biology Institute. IBMP, Curitiba, Brazil.

2014 - 2016 Microbe Molecular Biology. Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, Brazil

2009 - 2015 Diazotrophic Bacteria Genetics. Universidade Federal do Paraná, UFPR, Curitiba, Brazil.

2009 - 2016 Biological Nitrogen Fixation. Universidade Federal do Paraná, UFPR, Curitiba, Brazil

2009 - 2016 Science and Technology of Biological Nitrogen Fixation National Institute (INCT). Universidade Federal do Paraná, UFPR, Curitiba, Brazil

Complementary Education

2015 Capilar electroforesis (Credit Hours: 48h).

FIOCRUZ, Brazil.

2007 Basis of education. (Credit Hours: 48h).

Faculdade Jaguaraíva, FAJAR, Brazil.

2005 FISH: Hybridization Tchnique (Credit Hours: 8h).

Universidade Estadual de Ponta Grossa, UEPG, Brazil.

Expertise Areas

1. Molecular Biology

2. Biochemistry of Microorganisms

3. Agricultural Microbiology

4. Science and Food Technology

Languages

English: Understands Well, Speaks Well, Writes Well, Reads Well

Spanish: Understands Well, Speaks Well, Writes Well, Reads Well

French: Understands Well, Speaks Reasonably, Writes Reasonably, Reads Well

Portuguese: Understands Well, Speaks Well, Writes Well, Reads Well

Papers

1. *GRAZIANI SILVA, A. C., STETS, M. I., KALB LOPES, A. L., SCHLUGA, P. H. C., MARTON, S., MENDES, I. F., ANDRADE, A. S. R., KRIEGER, M. A., CARDOSO, J. Evaluation of anti-peptidoglycan aptamers bind a wide range of bacterial agents of sepsis. (Submitted).

2. *KALB LOPES, A. L., CARDOSO, J., SANTOS, F. R. C. C., GRAZIANI SILVA, A. C., STETS, M. I., ZANCHIN, N. I. T., SOARES, M. J., KRIEGER, M. A. Development of an immunomagnetic separation method to capture sepsis associated bacteria in blood. Journal of Microbiological Methods, v. 128, p. 96–101, 2016.

3. *STETS, M. I., ALQUERES, S., SOUZA, E. M., PEDROSA, F. O., SCHMID, M, HARTMANN, A., CRUZ, L. M. Quantification of Azospirillum brasilense FP2 in wheat roots by strain-specific qPCR. Applied and Environmental Microbiology, v. 81, n. 81, p. 6700 - 6709, 2015.

4. DALLAGASSA, C. B., HUERGO, L. F., STETS, M. I., PEDROSA, F. O., SOUZA, E. M., CRUZ, L. M., ASSIS, F. E. A., WOLF, S., VOLANSKI, W., PICHETH, G., PIGATTO, C. P., FARAH, S. M., FADEL-PICHETH, C. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories. Genetics and Molecular Research, v.13, p.716 - 722, 2014.

5. *STETS, M. I., ETTO, R. M., GALVÃO, C. W., AYUB, R., CRUZ, L. M., STEFFES, M. B. R., BARANA, A. C. Microbial community and performance of slaughterhouse wastewater treatment filters. Genetics and Molecular Research, v.13, p.4444 - 4455, 2014.

6. *STETS, M. I., PINTO JUNIOR, A. S., HUERGO, L. F., SOUZA, E. M., GUIMARAES, V. F., ALVESC, A. C., STEFFES, M. B. R., MONTEIRO, R. A., PEDROSA, F. O., CRUZ, L. M. Rapid identification of bacterial isolates from wheat roots by high resolution whole cell MALDI-TOF MS analysis. Journal of Biotechnology, v.165, p.167 - 174, 2013.

7. SILVA, T. M., STETS, M. I., MAZZETTO, A. M., ANDRADE, F. D., PILEGGI, S. A. V., FÁVERO, P. R., CANTÚ, M., CARRILHO, E., CARNEIRO, P. I. B., PILEGGI, M. Degradation of 2,4-D herbicide by microorganisms isolated from Brazilian contaminated soil. Brazilian Journal of Microbiology, v.38, p.522 - 525, 2007.

Poster published in conferences proceedings

1. FERRARI, G., STETS, M. I., CUNHA, A. M., GALVÃO, C. W., FURMAM, R. W., SANTOS, A. R. S., PETRY, V. E. K., ETTO, R. M., SOUZA, A. M., CHICORA, V. K., SOUZA, E. M., PEDROSA, F. O., GUIMARAES, V. F., CRUZ, L. M. MALDI-TOF grouping and biochemical analysis of isolated bacteria from soil and roots of wheat plants inoculated with the diazotrophic bacteria Azospirillum brasilense AbV5 In: XLll Reunião Anual da SBBq, 2013.

2. STETS, M. I., ALQUERES, S. M. C., CAMILIOS, N. D., ROTHBALLER, M., SCHMIDT, M., SOUZA, E. M., STEFFES, M. B. R., PEDROSA, F. O., CRUZ, L. M., HARTMANN, A. Monitoring population of Azospirillum brasilense FP2 inoculated in wheat roots by qPCR In: 13th Symposium on Biological Nitrogen Fixation with Non-Legumes, 2012.

3. WASSEN, R., CAMILIOS, N. D., TADRA-SFEIR, M. Z., BRUZAMARELLO-SANTOS, L. C., DONATTI, L., STETS, M. I., WEISS, V., FAORO, H., CHUBATSU, L. S., MONTEIRO, R. A., PEDROSA, F. O., SOUZA, E. M. Transcription profiling of wheat (Triticum aestivum) roots colonized by Azospirillum brasilense. 13th Symposium on Biological Nitrogen Fixation with Non-Legumes, 2012.

4. SANTOS, R. S., STETS, M. I., HUERGO, L. F., SOUZA, W. E., SOUZA, E. M., PEDROSA, F. O., NEVES, L. A. P., CRUZ, L. M. MS-Analyser: a whole-cell mass spectrometry analysis system for microorganisms clusterization and identification In: 8 th International Conference of the Brazilian Association for Bioinformatics and Computational Biology X-meeting, 2012.

5. CHICORA, V. K., STETS, M. I., RIGO, L. U., STEFFENS, M. B. R., SOUZA, E. M., PEDROSA, F. O., CRUZ, L. M. Molecular analysis of Herbaspirillum and diazotrophic endophytes isolated from pineapple and banana. In: 19º Evento de Iniciação Científica, 2011.

6. STETS, M. I., ARAUJO, L. M., SILVA-FROUFE, L. G., HUERGO, L. F., GUIMARAES, V. F., SOUZA, E. M., STEFFENS, M. B. R., PEDROSA, F. O., CRUZ, L. M. Analysis of wheat bacterial diversity by MALDI-TOF mass spectrometry In: XL Annual Meeting of The Brazilian Biochemistry and Molecular Biology Society, 2011.

7. DALLAGASSA, C. B., MARQUES, A. C. Q., WOLF, S., HUERGO, L. F., STETS, M. I., SUREK, M., FARAH, S. M., LIPUMA, J., PEDROSA, F. O., FADEL-PICHETH, C. Bacterial characterization by mass spectrometry MALDI-TOF In: 38º Congresso Brasileiro de Análises Clínicas e 11º Congresso Brasileiro de Citologia Clínica, 2011.

8. CHICORA, V. K., STETS, M. I., RIGO, L. U., STEFFENS, M. B. R., SOUZA, E. M., PEDROSA, F. O., CRUZ, L. M. Comparative Analysis of the Genus Herbaspirillum and Taxonomic Characterization of Diazotrophic Isolates of Pineapple and Banana In: XL Annual Meeting of The Brazilian Biochemistry and Molecular Biology Society, 2011.

9. DALLAGASSA, C. B., SUREK, M., STETS, M. I., HUERGO, L. F., SOUZA, E. M., PEDROSA, F. O., VIZZOTTO, B. S., FARAH, S. M., PICHETH, G., FADEL-PICHETH, C. Identification of Aeromonas species by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) In: IFCC – WorldLab – EuroMedLab Berlin, 2011.

10. CHICORA, V. K., SOUZA, E. M., RIGO, L. U., PEDROSA, F. O., STETS, M. I., SILVA-FROUFE, L. G., STEFFENS, M. B. R., CRUZ, L. M. Comparative analysis of Herbaspirillum spp and taxonomic characterization of diazotrophic isolated from pineapple and banana. In: 18º Evento de Iniciação Científica, 2010.

11. STETS, M. I., ARAUJO, L. M., SILVA-FROUFE, L. G., GUIMARAES, V. F., HUERGO, L. F., SOUZA, E. M., PISA, G., SCHMIDT, M. A., PEDROSA, F. O., STEFFENS, M. B. R., CRUZ, L. M. Analysis of the Diversity of Wheat-associated Bacteria by MALDI-TOF Mass Spectrometry and 16S rRNA gene sequencing In: XXXIX Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2010.

12. STETS, M. I., ARAUJO, L. M., SILVA-FROUFE, L. G., GUIMARAES, V. F., HUERGO, L. F., SOUZA, E. M., PEDROSA, F. O., STEFFENS, M. B. R., CRUZ, L. M. Diversity of Wheat-Associated Bacteria by MALDI-TOF Mass Spectrometry and 16S rRNA Gene Sequencing In: 12th International Symposium on Biological Nitrogen Fixation with Non-Legumes and the 2th International INCT Symposium on Biological Nitrogen Fixation, 2010.

13. FAORO, H., BRUSAMARELLO, L. C. C., TADRA-SFEIR, M. Z., CAMILIOS, N. D., SCHWAB, S., STETS, M. I., CHICORA, V. K., SOUZA, E. M., STEFFENS, M. B. R., CHUBATSU, L. S., MONTEIRO, R. A., CRUZ, L. M., HUERGO, L. F., RIGO, L. U., WASSEN, R., FADEL-PICHETH, C., MULLER-SANTOS, M., DA-ROCHA, W. D., BONATTO, A. C., PEDROSA, F. O. Genomic Comparison of Herbaspirillum spp Species In: 12th International Symposium on Biological Nitrogen Fixation with Non-Legumes and the 2th International INCT Symposium on Biological Nitrogen Fixation, 2010.

14. DALLAGASSA, C. B., SOUZA, E. M., HUERGO, L. F., STETS, M. I., PICHETH, G., FADEL-PICHETH, C. Identification of clinical isolates using mass spectrometry MALDI-TOF In: 2º Simpósio Internacional de Microbiologia Clínica, 2010.

15. GOOD, M., AYUB, R., ETTO, R. M., STETS, M. I., STEFFENS, M. B. R., GALVÃO, C. W. Cloning and Expression of the Cucumis melo aco1 Gene cDNA In: XXXVIIl Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq), 2009.

16. STETS, M. I., ETTO, R. M., GALVÃO, C. W., AYUB, R., STEFFENS, M. B. R., CRUZ, L. M., PEDROSA, F. O., BARANA, A. C. Prokaryotic Diversity in Upflow Anaerobic Biodigestors Treating Slaughterhouse Waste In: XXXVIII Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq), 2009.

17. STETS, M. I., ETTO, R. M., GALVÃO, C. W., STEFFENS, M. B. R., BARANA, A. C.

Evaluation of performance and microbial community analysis of Upflow Anaerobic Filters treating slaughterhouse waste. In: XXXVII Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq) and XI Congress of the Pan American Association for Biochemistry and Molecular Biology (PABMB), 2008.

Technical Production

Courses offered

2013 STETS, M. I., CORDEIRO, F. A., SANTOS, M. M., HUERGO, L. F. I Biochemistry Scientific Week UFPR - Course: Analytical Methods in Proteomics. Duration: 35 hours.

2011 STETS, M. I., CORDEIRO, F. A., SANTOS, M. M., ALBERTON, D., CORDEIRO, D., HUERGO, L. F. II Winter Course of Biochemistry and Molecular Biology - Course: Introduction to Proteomics Analysis. Duration: 12 hours.

2010 STETS, M. I., CORDEIRO, F. A., CUNHA, E., HUERGO, L. F. I Winter Course of Biochemistry and Molecular Biology - Introduction to Proteomics Analysis: Principles and Applications. Duration: 12 hours.

Lectures

2013 STETS, M. I. Lecture: Molecular techniques in proteomics and real-time PCR. Location: Federal University of Paraná; City: Curitiba; Event: Seminar Course; Postgraduate in bioinformatics program.

2011 STETS, M. I. Lecture: Mass spectrometry for bacterial identification in biodiversity studies, clinical diagnosis and bioterrorism, Location: Ponta Grossa State University; City: Ponta Grossa; Event: XXIII Week Academic studies in Biology; Academic Center Erasmus Darwin - CAED.

2010 STETS, M. I. Diversity of wheat-associated bacteria by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. In: 12th International Symposium on Biological Nitrogen Fixation with Non-Legumes and the 2th International INCT Symposium on Biological Nitrogen Fixation.

Judging committee

Master

2013 STETS, M. I., SANTOS, M. A., CRUZ, L. M. Examination Board of Rodrigo Souza dos Santos. Thesis: MS Analyser: a system for microorganism identification in mass spectrometric analysis. (Posgraduate in Bioinformatics program). Federal University of Parana.

University

2015 GALVÃO, C. W., ETTO R. M., STETS, M. I. Participation In Examination Board of Rafaela Wiegand Furmam. Identification of microorganisms by mass spectrometry and 16S rRNA gene partial sequencing. (Pharmacy school) Ponta Grossa State University.

2013 CRUZ, L. M., STEFFES, M. B. R., STETS, M. I. Participation In Examination Board of Vanessa Kessler Chicora. Molecular analysis of Herbaspirillum spp and diazotrophic endophytes isolated of pineapple and banana. (Biological Sciences School) Federal University of Parana.

2011 STETS, M. I., GALVÃO, C. W., RIOS, E. M. Participation In Examination Board Leilane Caline Silva. Training defense. (Biological Sciences School) Ponta Grossa State University.

2010 BARANA, A. C., STETS, M. I., PUKASIEWICZ, S. Participation In Examination Board of Lis Maria Clotilde Galeano Rivas and Vanessa Leifeld. Evaluation of anaerobic biological filter for slaughterhouse wastewater treatment with different power systems and hydraulic retention time. (Food Engineering School) Ponta Grossa State University.

2009 GALVÃO, C. W., STETS, M. I., SILVEIRA, R. B. Participation In Examination Board of Maiara Good. Cloning of c-acco1 gene of Cucumis melo (Melon). (Food Engineering School) Ponta Grossa State University.

2008 GALVÃO, C. W., STETS, M. I., ETTO, R. M. Participation In Examination Board in Finished wok course of Gabrielle Amaral e Silva Muller. In silico analysis of Herbaspirullum seropedicae genes involved in DNA repair. (Biological Sciences School) Ponta Grossa State University.

Administrative functions in university

2010 – 2011 Student representative of the Biochemistry and Molecular Biology Department of Federal University of Parana.

Professional Experience

2010 – Actually Universidade Federal do Paraná, UFPR, Brazil.

Activities: Project participation activities.

Research Projects: Biological Nitrogen Fixation;

Diazotrophic Bacteria Genetics;

Science and Technology of Biological Nitrogen Fixation National Institute (INCT).

2010 – 2010 Pontifícia Universidade Católica do Paraná, PUC/PR, Brazil.

Biotechnology Professor, Credit Hours: 15

2007 – 2008 Instituto de Educação Contemporânea á Distância, IECAD, Brazil.

Biology Professor, Credit Hours: 6 hours/week

2006 – 2007 Centro Educacional de Niteroi, CEN*, Brazil.

Biology Professor, Credit Hours: 6 hours/week

2006 - Actually Universidade Estadual de Ponta Grossa, UEPG, Brazil.

Activities: Project participation activities.

Research Project: Microbial molecular biology

2009 – Actually Empresa Brasileira de Pesquisa Agropecuária, EMBRAPA, Brazil.

Activities: Project participation activities.

Research Project: Diazothrophics bacteria genetics.

Student supervision

2015 Marina Soneghett Cotta. Monitoring Azospirillum brasilense Abv5 strain inoculation in wheat and corn crops. 2015. Master degree (Science (Bioquemistry). - Universidade Federal do Paraná.

2014 Roxana Beatriz Ribeiro Chaves. Identification of Plant-Bacteria Association by mass spectrometry and a bioinformatics database creation. 2014. Master degree (Science (Bioquemistry). - Universidade Federal do Paraná.

2011 Leilane Caline Silva. Technical supervision of compulsory training. 2011. Universidade Estadual de Ponta Grossa.

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