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microbiology

Location:
Tamil Nadu, India
Posted:
October 22, 2016

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Saranya Sathishkumar

**/**, **********, *****

Phone Number: (+91)99943-07349. Email: acw6h7@r.postjobfree.com

EDUCATION:

Higher Secondary Education

Vetri Vikaas Higher Secondary School,

Mallur, Namakkal.

Bachelor of Technology in Biotechnology May 2013 CGPA: 86.7

K.S.Rangasamy College of Technology,

Tiruchengode, Namakkal

P.G. Diploma in Cellular and Molecular Diagnostics October 2016

Manipal University,

Manipal.

PROJECT DESCRIPTIONS:

B.Tech., PROJECT WORK

AN IMPROVED METHOD FOR Agrobacterium MEDIATED GENETIC TRANSFORMATION FROM COTYLEDON EXPLANTS OF

Solanum melongena L.

Published In: (International Journal of Current Microbiology and Applied Sciences)

K.S.Rangasamy College of Technology Tiruchengode.

Brinjal is a tropical vegetable cultivated throughout the tropical regions of India. The varieties of Solanum melongena L. display a wide range of fruit shapes and colors. An experiment was conducted for the production of tolerant variety with the native variety of brinjal cv. Valuthalai produce long and white coloured fruits. The callus regeneration efficiency of tender shoot tip, hypocotyls, leaf and stem explant were analysed using MS basal media supplemented with different combinations and concentrations of phytohormones. The frequency of callus induction for shoot tip, hypocotyls, leaves, stem explant were 76.43%, 81.56%, 74.37% and 84.92% respectively and the maximum proliferation found at 0.6mg/l 2, 4-D. The maximum efficiency of multiple shooting was obtained at 0.2mg/l BAP and 0.6mg/l NAA and o.4 mg/l IAA. The successful rooting was recorded on media supplemented with 0.4mg/l of IBA.

The regenerated plant shoot tips and callus were infected with Agrobacterium tumefaciens by co-cultivation process. The infected callus was regenerated in the 250mg/l kanamycin and 300mg/l cefatoxime effectively with the time duration of 52 hours. The transformed plantlets with the T-DNA insertion were determined with the rate of germination.

Mini project :

BIOLEACHING STUDIES OF SOLUBLE PHOSPHORUS FROM ROCK PHOSPHATE

GOT ISSN NUMBER

A novel approach to bioleach phosphorus from rock phosphate, involving the bio-reduction of sulfur by adapted Thiobacillus ferrooxidans (NCIM 5370) and the product of sulfuric acid to dissolve rock phosphate, has been proposed in this paper. The initial pH for the growth of microbe was 2.0.At different concentrations of pulp density from 1% to 5%, the maximum of 12% phosphorus was leached in 1% pulp density concentration. The maximum of 14% phosphorus from 0.75% was leached at different sulphur concentration varying of 0.25% to 1.25%. By using various parameters such as pH versus time, ROP versus time, sulfate production versus time, the maximum amount of phosphorus leached was estimated. Time taken to determine various parameter analogs were 20 days. The particle size of rock phosphate was 0.05mm. Phosphorus leached was estimated by Barton's reagent involving the action of Ammonium metavanadate and Ammonium molybdate. The soluble phosphorus could be leached more effectively in the presence of sulfur by Thiobacillus ferrooxidans. The bacterium should be appropriately adapted with sulphur, which makes easier to bioleach soluble phosphorus from rock phosphate.

Major project:

PROBIOTICS/MICROBIAL METABOLITES ON STEATOSIS

Hepatic steatosis or nonalcoholic fatty liver disease (NAFLD) is an infiltration of fat inside hepatocytes, usually exceeding 5% of the liver wet weight. Three probiotics which were used for the study includes Lactobacillus plantarum, Lactobacillus rhamnosus and Bacillus subtilis. The aim of the study was to determine the effect of probiotics against steatohepatitis using HepG2 cell line.Microbial metabolites were treated against triglyceride induced hepatocytes to determine the rate of lipid reduction. From the growth kinetics data, the microbial cultures were incubated for 24 – 48 hours of incubation for the extraction.

Late log phase to stationary phase cells exhibit bioactive metabolite insight for their maximum activity. From the Bradford assay, it was found that the concentration of the protein which was extracted from Bacillus subtilis was about 500mg/ml and from Lactobacillus plantarum, Lactobacillus rhamnosus it was about 125mg/ml determined from the standard curve. Various concentrations of BSA: OA were used to optimize the efficient concentration of hepatic steatosis in vitro for the further study. It was observed from the Oil-Red-O staining, that 2mMOA:1mM BSA induced higher lipid accumulation.

Cell free supernatants of Bacillus subtilis showed 30.79% whereas Lactobacillus plantarum showed 25% and Lactobacillus rhamnosus showed 15% reduction in lipid accumulation from the 5ul of sample. Among the three probiotics, acetone precipitated sample from B.subtilis showed maximum reduction in triglyceride accumulation which constitutes about 38% while 20% reduction in L.plantarum and 27% in L.rhamnosus of their nontoxic concentration chosen. From the cell lysates, the lipid reduction was about 56% in the 10ul of B.subtilis sample. It was about 36% reduction in 5ul of L.plantarum and 53% in 50ul of L.rhamnosus cell lysates. From the overall observation, it was confirmed that B.subtilis metabolites were efficient in the reduction of hepatic steatosis.

PUBLICATION:

Abstract Published in Indian engineering congress 2011, Maheshwari, Saranya, Ayyappadasan.G, venkatesa prabhu.S, Rojin George, “Invitro mass cultivation of garlic for the production of allicin using suspension culture”

TECHNICAL SYMPOSIUM AND CONFERENCES:

•Awarded I prize for paper presentation, product launch, Quiz held in 12th ISTE chamber of Adhiparasakthi Engineering College.

•Awarded I prize for technical paper presentation in the topic of Studies on Bioleaching of phosphorus from rock phosphate held at Sri Nandhanam College of Engineering and Technology.

•Participated in various workshop, conferences, project expo and in-plant training for Advanced molecular techniques, medical coding at K.S.Rangasamy College of Technology, SASTRA University and certain Government institutions.

•Participated and won various prizes for technical presentation held at various institutes like

SRM University, Vel Tech High Tech College, Coimbatore Institute of Technology, Government College of Technology, K.S.Rangasamy College of Technology.

•Got stipend from Department of Biotechnology, Government of Karnataka to pursue Diploma in Cellular and Molecular Diagnostics.

•Got an opportunity to do my project in The Himalaya Drug Company, Bangalore as part of the P.G. Diploma program and got job confirmation.

PROFILE OF TECHNICAL QUALIFICATIONS:

•Molecular biology: Gel electrophoresis of DNA, RNA and proteins, flow cytometry, construction of recombinant DNA, PCR techniques, automated karyotyping (C banding, G banding, NOR banding), chromosomal aberations in mice, ELISA, automated DNA sequencing, SSCP

•Biochemical assays: Estimation of carbohydrates, lipids, proteins and Nucleic acids.

Basic biochemical assays.

• Culturing tech.: Microbial culturing, Plant Tissue culture, cell line culture

PERSONAL INFORMATION:

Husband’s name : R. SathishKumar

Age & DOB : 09.02.1992&24

Sex : Female

Nationality : Indian

Languages Known: English & Tamil

Strength : optimistic, motivated and determined, capability to work in various circumstances



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