Olivia Steffen Buffo
* ***** ******** *** *** M*J3K9 Toronto, ON acw0hi@r.postjobfree.com/ 647-***-****
OBJECTIVE: LABORATORY TECHNICIAN
To obtain a placement into a laboratory where I can demonstrate my knowledge and skills obtained throughout my career, and to further my work experience in a team setting while developing new abilities.
EDUCATION
B. Sc. in Biology
University of São Paulo (USP), Brazil January - 2013
Health Technician
Tech School of Campinas (UNICAMP), Brazil December - 2003
CORE STRENGTHS
Laboratory Proficiencies:
Cellular and Molecular Biology Techniques
Laboratory Management
General laboratory Cleanup
WHMIS
Specimen Processing
Sterilization
Reports
Special Skills:
Ability to work individual and in a team environment
Problem- solving
Great Team Worker
Good Laboratory Practice
Bilingual: Portuguese-English
Microsoft Office Suite
Ability to quickly learn new skills and techniques
RELEVANT WORK EXPERIENCE
Laboratory Technician
School of Veterinary Medicine, University of São Paulo (USP), Brazil February 2013 – March 2015
Conducted daily laboratory routines including cleaning and sterilizing equipment, purchasing materials, and prepped solutions for running tests
Performed cellular and molecular biology procedures (RNA extraction, RT-PCR, Gel electrophoresis, Western Blotting, etc.)
Coordinated the storage and disposal of biological specimens and chemical material
Performed autopsy in small, medium and large sized animals to determine the cause of death.
Assisted in autopsy service and performed postmortem examinations
Prepared microscope slides for university research, diagnosis of biopsies, and necropsy reports -The Paraffin Method and Hematoxylin and Eosin (H&E) Staining)
Assisted and taught residents and graduate students in autopsy techniques
Analyze data and prepared reports for senior researchers and professors
Laboratory Assistant
School of Veterinary Medicine, University of São Paulo (USP), Brazil January 2012 – February 2013
Washed, sterilized, and stocked ordinary glassware
Updated the purchasing inventory database, stocked the labs with supplies
Collected biological waste from the laboratories and prepared the waste for collection
Assisted the lab technicians and the researchers on the laboratory routines
Sterilize laboratory supplies and reagents by using autoclave
Student Researcher (Part-Time) July 2009 – July 2011 University of São Paulo (USP) - Science and Research Project - (The frequency of occurrence of the avian infectious bronchitis virus (IBV) through RT-PCR in different internal organs)
Achieved an honorable mention in the project select among over 4 thousand different projects.
Collected biological material in the field (Animal Farms)
Processed collected material in the lab using RNA extraction techniques, RT-PCR, and electrophoresis
Maintained of a safe and clear laboratory environmental
Diagnosed the occurrence of the IBV virus in chickens and quails in order to establish the distribution of the virus in the main producing poultry farms the state of Sao Paulo
Prepared reagents, buffers, and others solutions
ADDITIONAL EXPERIENCE
Barista/Cashier/Customer Service - Canadian Work Experience
Delysees – Toronto, ON September 2016 - Current
Customer Service Order and Coffee/Tea Preparation
Maintaining of customer service and Café quality and cleanliness standards
Preparing/Serving food & beverage
Quality customer service
Cash duties
Receiving, putting orders away and stocks
Ensure that all responsibilities and paperwork are completed in a timely manner
Various cleaning duties
Health & Safety (WHMIS)
Maintaining a safe, clean & hygienic environment
Assisting in various other duties
Teacher/ Tutor (Part-Time)
Learning and Stimulus Center (NAE) – São Paulo, Brazil June 2015 – June 2016
Held private lessons for high school students in biology, mathematics, physics and chemistry
Taught different techniques for learning and memorization
Provided support for students with learning disabilities
Trained and prepared students for the Brazilian SATs