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Laboratory Technician - Biology

Toronto, Ontario, Canada
October 11, 2016

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Olivia Steffen Buffo

* ***** ******** *** *** M*J3K9 Toronto, ON 647-***-****


To obtain a placement into a laboratory where I can demonstrate my knowledge and skills obtained throughout my career, and to further my work experience in a team setting while developing new abilities.


B. Sc. in Biology

University of São Paulo (USP), Brazil January - 2013

Health Technician

Tech School of Campinas (UNICAMP), Brazil December - 2003


Laboratory Proficiencies:

Cellular and Molecular Biology Techniques

Laboratory Management

General laboratory Cleanup


Specimen Processing



Special Skills:

Ability to work individual and in a team environment

Problem- solving

Great Team Worker

Good Laboratory Practice

Bilingual: Portuguese-English

Microsoft Office Suite

Ability to quickly learn new skills and techniques


Laboratory Technician

School of Veterinary Medicine, University of São Paulo (USP), Brazil February 2013 – March 2015

Conducted daily laboratory routines including cleaning and sterilizing equipment, purchasing materials, and prepped solutions for running tests

Performed cellular and molecular biology procedures (RNA extraction, RT-PCR, Gel electrophoresis, Western Blotting, etc.)

Coordinated the storage and disposal of biological specimens and chemical material

Performed autopsy in small, medium and large sized animals to determine the cause of death.

Assisted in autopsy service and performed postmortem examinations

Prepared microscope slides for university research, diagnosis of biopsies, and necropsy reports -The Paraffin Method and Hematoxylin and Eosin (H&E) Staining)

Assisted and taught residents and graduate students in autopsy techniques

Analyze data and prepared reports for senior researchers and professors

Laboratory Assistant

School of Veterinary Medicine, University of São Paulo (USP), Brazil January 2012 – February 2013

Washed, sterilized, and stocked ordinary glassware

Updated the purchasing inventory database, stocked the labs with supplies

Collected biological waste from the laboratories and prepared the waste for collection

Assisted the lab technicians and the researchers on the laboratory routines

Sterilize laboratory supplies and reagents by using autoclave

Student Researcher (Part-Time) July 2009 – July 2011 University of São Paulo (USP) - Science and Research Project - (The frequency of occurrence of the avian infectious bronchitis virus (IBV) through RT-PCR in different internal organs)

Achieved an honorable mention in the project select among over 4 thousand different projects.

Collected biological material in the field (Animal Farms)

Processed collected material in the lab using RNA extraction techniques, RT-PCR, and electrophoresis

Maintained of a safe and clear laboratory environmental

Diagnosed the occurrence of the IBV virus in chickens and quails in order to establish the distribution of the virus in the main producing poultry farms the state of Sao Paulo

Prepared reagents, buffers, and others solutions


Barista/Cashier/Customer Service - Canadian Work Experience

Delysees – Toronto, ON September 2016 - Current

Customer Service Order and Coffee/Tea Preparation

Maintaining of customer service and Café quality and cleanliness standards

Preparing/Serving food & beverage

Quality customer service

Cash duties

Receiving, putting orders away and stocks

Ensure that all responsibilities and paperwork are completed in a timely manner

Various cleaning duties

Health & Safety (WHMIS)

Maintaining a safe, clean & hygienic environment

Assisting in various other duties

Teacher/ Tutor (Part-Time)

Learning and Stimulus Center (NAE) – São Paulo, Brazil June 2015 – June 2016

Held private lessons for high school students in biology, mathematics, physics and chemistry

Taught different techniques for learning and memorization

Provided support for students with learning disabilities

Trained and prepared students for the Brazilian SATs

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